Korean J Hematol 1999; 34(3):
Published online September 30, 1999
© The Korean Society of Hematology
양동욱, 홍원표, 조덕, 신종희, 서순팔, 이제중, 정익주, 조문기, 국훈, 황태주
전남대학교 의과대학 임상병리학교실,
전남대학교 의과대학 내과학교실,
전남대학교 의과대학 소아과학교실
BACKGROUND: Interphase fluorescence in situ hybridization (FISU) analysis following sex-mismatched bone marrow transplantation (BMT) is a sensitive and quantitative method to better assess the engraftment state and mixed chimerism.
METHODS: Twelve patients (allogeneic 11 cases, unrelated 1 case) who underwent sex-mismatched BMT at Chonnam University Hospital from April 1996 through October 1998 were anlaysed chimerism employing FISH. Interphase FISH studies on peripheral blood cytospin slides were performed by using chromosome X α-satellite probe in early post -transplant periods at intervals of 3 days and in follow-up periods 6∼15 months after BMT.
RESULTS: In 11 engrafted patients, the mean percentage of host cells was 3.0% at the period of engraftment (15∼25 days). Follow-up interphase FISH studios for ten patients with hematologic remission states after engraftment showed mixed chimerism with variable degree (mean, 4.7%: ranges, 0.5∼19.0%). Although three of these patients showed host cells above 5.0% (13.2, 8.0 and 7.0%) transiently, they maintained hematologic remission states. One patient who showed 8.5% (344 days) and 14.5% (596 days)
host cells proved to be an engraftment failure.
CONCLUSION: Engrafted patients who obtained hematological remission showed variable mixed chimerism by FISH. When the host cells were low rate or increased transiently, mixed chimerism was not related to relapse. However, consecutive increasing host cells suggested engraftment failure or relapse. More sensitive long term follow-up FISH studies will help to evaluate and monitor engraftment status and degree of chimerism.
Keywords Bone marrow transplantation, FISH, Engraftment, Mixed chimerism
Korean J Hematol 1999; 34(3): 461-470
Published online September 30, 1999
Copyright © The Korean Society of Hematology.
양동욱, 홍원표, 조덕, 신종희, 서순팔, 이제중, 정익주, 조문기, 국훈, 황태주
전남대학교 의과대학 임상병리학교실,
전남대학교 의과대학 내과학교실,
전남대학교 의과대학 소아과학교실
Dong Wook Ryang, Won Pyo Hong, Deok Cho, Jong Hee Shin, Soon Pal Suh, Je Jung Lee, Ik Joo Chung, Moon Kee Cho, Hoon Kook, Tai Ju Hwang
Department of Clinical Pathology, Internal Medicine, Pediatrics, Chonnam University Medical School, Kwangju, Korea
Department of Internal Meddicine, Korea
Department of Pediatrics, Korea
BACKGROUND: Interphase fluorescence in situ hybridization (FISU) analysis following sex-mismatched bone marrow transplantation (BMT) is a sensitive and quantitative method to better assess the engraftment state and mixed chimerism.
METHODS: Twelve patients (allogeneic 11 cases, unrelated 1 case) who underwent sex-mismatched BMT at Chonnam University Hospital from April 1996 through October 1998 were anlaysed chimerism employing FISH. Interphase FISH studies on peripheral blood cytospin slides were performed by using chromosome X α-satellite probe in early post -transplant periods at intervals of 3 days and in follow-up periods 6∼15 months after BMT.
RESULTS: In 11 engrafted patients, the mean percentage of host cells was 3.0% at the period of engraftment (15∼25 days). Follow-up interphase FISH studios for ten patients with hematologic remission states after engraftment showed mixed chimerism with variable degree (mean, 4.7%: ranges, 0.5∼19.0%). Although three of these patients showed host cells above 5.0% (13.2, 8.0 and 7.0%) transiently, they maintained hematologic remission states. One patient who showed 8.5% (344 days) and 14.5% (596 days)
host cells proved to be an engraftment failure.
CONCLUSION: Engrafted patients who obtained hematological remission showed variable mixed chimerism by FISH. When the host cells were low rate or increased transiently, mixed chimerism was not related to relapse. However, consecutive increasing host cells suggested engraftment failure or relapse. More sensitive long term follow-up FISH studies will help to evaluate and monitor engraftment status and degree of chimerism.
Keywords: Bone marrow transplantation, FISH, Engraftment, Mixed chimerism
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