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Original Article

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Blood Res 2018; 53(4):

Published online December 31, 2018

https://doi.org/10.5045/br.2018.53.4.320

© The Korean Society of Hematology

Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings

Abdolrahim Ahmadi1, Saeid Kaviani1,#*, Marjan Yaghmaie2,3,4,#*, Hossein Pashaiefar2,3,4, Mohammad Ahmadvand2,3,4, Mahdi Jalili2,3,4, Kamran Alimoghaddam2,3,4, Mohammad Eslamijouybari5, and Ardeshir Ghavamzadeh2,3,4

Author Info. +

1Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.

2Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran, Iran.

3Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran, Iran.

4Hematologic Malignancies Research Center, Tehran University of Medical Sciences, Tehran, Iran.

5Comprehensive Cancer Research Center, Mazandaran University of Medical Science, Sari, Iran.

Correspondence to : Correspondence to Saeid Kaviani, M.D. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, P.O.Box: 14115-331, kavianis@modares.a.ir

Received: June 7, 2018; Revised: August 27, 2018; Accepted: September 5, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Background

Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.

Methods

MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH).

Results

MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients.

Conclusion

Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.

Keywords MALAT1, Chronic lymphocytic leukemia, qRT-PCR, FISH

Article

Original Article

Blood Res 2018; 53(4): 320-324

Published online December 31, 2018 https://doi.org/10.5045/br.2018.53.4.320

Copyright © The Korean Society of Hematology.

Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings

Abdolrahim Ahmadi1, Saeid Kaviani1,#*, Marjan Yaghmaie2,3,4,#*, Hossein Pashaiefar2,3,4, Mohammad Ahmadvand2,3,4, Mahdi Jalili2,3,4, Kamran Alimoghaddam2,3,4, Mohammad Eslamijouybari5, and Ardeshir Ghavamzadeh2,3,4

1Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.

2Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran, Iran.

3Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran, Iran.

4Hematologic Malignancies Research Center, Tehran University of Medical Sciences, Tehran, Iran.

5Comprehensive Cancer Research Center, Mazandaran University of Medical Science, Sari, Iran.

Correspondence to:Correspondence to Saeid Kaviani, M.D. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, P.O.Box: 14115-331, kavianis@modares.a.ir

Received: June 7, 2018; Revised: August 27, 2018; Accepted: September 5, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.

Methods

MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH).

Results

MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients.

Conclusion

Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.

Keywords: MALAT1, Chronic lymphocytic leukemia, qRT-PCR, FISH

Fig 1.

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Figure 1.

Relative expression of MALAT1 (2−Δct) in CLL and control group using β2m as internal control gene. Increased expression of MALAT1 was observed in CLL patients (P=0.008).

Blood Research 2018; 53: 320-324https://doi.org/10.5045/br.2018.53.4.320
Demographic and clinical information on study participants.

a)Clinical stage of CLL patients according to Rai staging system..

Abbreviations: CLL, chronic lymphocytic leukemia; WBC, White blood cells..
<italic>MALAT1</italic> expression levels in the different FISH prognosis categories.

Good: 13q14.3 (sole abnormality); intermediate: trisomy 12 and normal karyotype; Poor: 17p13.1 and 11q22.3..
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