Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia

Davod Javanmard; Masood Ziaee; Hadi Ghaffari; Mohammad Hasan Namaei; Ahmad Tavakoli; Hamidreza Mollaei; Mohsen Moghoofei; Helya Sadat Mortazavi; Seyed Hamidreza Monavari

doi:10.5045/br.2017.52.4.311

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Original Article

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Blood Res 2017; 52(4):

Published online December 31, 2017

https://doi.org/10.5045/br.2017.52.4.311

Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia

Davod Javanmard¹, Masood Ziaee², Hadi Ghaffari¹, Mohammad Hasan Namaei², Ahmad Tavakoli¹, Hamidreza Mollaei³, Mohsen Moghoofei^4,5, Helya Sadat Mortazavi¹, and Seyed Hamidreza Monavari^1*

Author Info. +

¹Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

²Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.

³Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.

⁴Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

⁵Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.

Correspondence to : Seyed Hamidreza Monavari, Ph.D. Department of Virology, School of Medicine, Iran University of Medical Sciences, Hemmat Exp. way, Tehran, Iran. hrmonavari@yahoo.com

Received: April 28, 2017; Revised: May 15, 2017; Accepted: June 14, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Abstract

Background

Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resistance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovirus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran.

Methods

This was a cross-sectional epidemiological study comprising nearly all people with hemophilia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time polymerase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR.

Results

In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients (P <0.05).

Conclusion

There was a high prevalence of B19V and PARV4 infection in this high-risk group of patients with hemophilia. Due to the clinical significance of the B19 virus, imposing more precautionary measures for serum and blood products is recommended.

Keywords Parvovirus B19, PARV4, Hemophilia, Prevalence, Iran

Article

Original Article

Blood Res 2017; 52(4): 311-315

Published online December 31, 2017 https://doi.org/10.5045/br.2017.52.4.311

Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia

Davod Javanmard¹, Masood Ziaee², Hadi Ghaffari¹, Mohammad Hasan Namaei², Ahmad Tavakoli¹, Hamidreza Mollaei³, Mohsen Moghoofei^4,5, Helya Sadat Mortazavi¹, and Seyed Hamidreza Monavari^1*

¹Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

²Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.

³Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran.

⁴Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

⁵Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.

Correspondence to:Seyed Hamidreza Monavari, Ph.D. Department of Virology, School of Medicine, Iran University of Medical Sciences, Hemmat Exp. way, Tehran, Iran. hrmonavari@yahoo.com

Received: April 28, 2017; Revised: May 15, 2017; Accepted: June 14, 2017

Abstract

Background

Methods

Results

Conclusion

Keywords: Parvovirus B19, PARV4, Hemophilia, Prevalence, Iran

Abstract

Fig 1.

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Figure 1.

Result of PCR for PARV4 produced fragments of 490 base pairs.

Blood Research 2017; 52: 311-315https://doi.org/10.5045/br.2017.52.4.311

Table 1 Demographic, clinical, and virological characteristics of patients with hemophilia.

^a)There was a significant difference in B19V and PARV4 according to severity of hemophilia, between the moderate and mild groups..

^b)There was a significantly greater prevalence of B19V among participants aged 20–40 years, but a significant PARV4 prevalence in those aged <20 years, compared with other age groups..

Abbreviations: B19V, human parvovirus B19; HCV, hepatitis C virus; N, number; PARV4, human parvovirus 4..

Table 2 Characteristics of patients positive for B19V and PARV4.

Abbreviations: B19V, human parvovirus B19; HCV, hepatitis C virus; N, number; PARV4, human parvovirus 4..