Korean J Hematol 1997; 32(2):
Published online June 30, 1997
© The Korean Society of Hematology
재생불량성 빈혈 환자에서 혈청 Stem Cell Factor (SCF)치 및 재조합 인 SCF의 조혈집락세포 성장에 대한 영향
조덕연, 김철희, 배광봉, 김현수, 박상준, 김종숙, 최지영, 윤환중, 김삼용
충남대학교 의과대학 내과학교실
Serum Stem Cell Factor(SCF) Levels and In Vitro Response of Hematopoietic Progenitors to Recombinant Human SCF(rhSCF) in Patients with Aplastic Anemia
Background: Stem cell factor(SCF), which is produced from stromal cells of bone marrow, is a growth factor acting in early stages of hematopoiesis. Bone marrow failure in aplastic anemia seems to be mainly due to hematopoietic stem cell defect. However, there has been some evidence that microenvironmental defects contribute to the pathophysiology of this disorder.
Methods: We measured serum SCF levels in 29 patients with aplastic anemia at diagnosis using ELISA to define the status of soluble SCF in this disorder. We also
examined the effect of serum from 15 patients with aplastic anemia on the colony growth(CFU-GM and BFU-E) of normal bone marrow cells and the ability of recombinant human SCF to stimulate in vitro colony growth of bone marrow cells from 10 patients with aplastic anemia.
Results: Levels of serum SCF in patients with aplastic anemia were not different from those in 25 healthy controls(1,493±392pg/mL vs. 1,563±322pg/mL) or those in patients
with idiopathic thrombocytopenic purpura, acute myeloid leukemia, and myelodysplastic syndrome. No correlation was found between serum SCF levels and hematologic
parameters at diagnosis such as neutrophil count, hemoglobin, and platelet count. No difference was noted in the serum SCF levels according to severity of the disease. The effect of serum from patients with aplastic anemia on the colony growth of normal bone marrow cells were variable and were not correlated with serum SCF levels. rhSCF increased the colony numbers of bone marrow cells from patients with aplastic anemia.
Conclusion: These results indicate that soluble SCF is not deficient in aplastic anemia, but SCF can stimulate the proliferation of bone marrow cells from aplastic anemia.
Keywords Aplastic anemia; Serum stem cell factor; Colony growth;
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Korean J Hematol 1997; 32(2): 203-213
Published online June 30, 1997
Copyright © The Korean Society of Hematology.
재생불량성 빈혈 환자에서 혈청 Stem Cell Factor (SCF)치 및 재조합 인 SCF의 조혈집락세포 성장에 대한 영향
조덕연, 김철희, 배광봉, 김현수, 박상준, 김종숙, 최지영, 윤환중, 김삼용
충남대학교 의과대학 내과학교실
Serum Stem Cell Factor(SCF) Levels and In Vitro Response of Hematopoietic Progenitors to Recombinant Human SCF(rhSCF) in Patients with Aplastic Anemia
Deog Yeon Jo, Chul Hee Kim, Kwang Bong Bae, Hyun Soo Kim, Sang Jun Park, Jong Suk Kim, Jee Young Choi, Hwan Jung Yun, Sam Yong Kim
Department of Internal Medicine, Chungnam National University College of Medicine, Taejon, Korea
Abstract
Background: Stem cell factor(SCF), which is produced from stromal cells of bone marrow, is a growth factor acting in early stages of hematopoiesis. Bone marrow failure in aplastic anemia seems to be mainly due to hematopoietic stem cell defect. However, there has been some evidence that microenvironmental defects contribute to the pathophysiology of this disorder.
Methods: We measured serum SCF levels in 29 patients with aplastic anemia at diagnosis using ELISA to define the status of soluble SCF in this disorder. We also
examined the effect of serum from 15 patients with aplastic anemia on the colony growth(CFU-GM and BFU-E) of normal bone marrow cells and the ability of recombinant human SCF to stimulate in vitro colony growth of bone marrow cells from 10 patients with aplastic anemia.
Results: Levels of serum SCF in patients with aplastic anemia were not different from those in 25 healthy controls(1,493±392pg/mL vs. 1,563±322pg/mL) or those in patients
with idiopathic thrombocytopenic purpura, acute myeloid leukemia, and myelodysplastic syndrome. No correlation was found between serum SCF levels and hematologic
parameters at diagnosis such as neutrophil count, hemoglobin, and platelet count. No difference was noted in the serum SCF levels according to severity of the disease. The effect of serum from patients with aplastic anemia on the colony growth of normal bone marrow cells were variable and were not correlated with serum SCF levels. rhSCF increased the colony numbers of bone marrow cells from patients with aplastic anemia.
Conclusion: These results indicate that soluble SCF is not deficient in aplastic anemia, but SCF can stimulate the proliferation of bone marrow cells from aplastic anemia.
Keywords: Aplastic anemia, Serum stem cell factor, Colony growth,
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