Korean J Hematol 1997; 32(2):
Published online June 30, 1997
© The Korean Society of Hematology
제대혈에서의 CD34+세포 및 아형과 CFU-GM과의 상관관계
배광렬, 이영호, 박상수, 김은정, 김태홍, 최안홍, 김태겸, 김경희, 한진영, 김정만
동아대학교 의과대학 소아과학교실,
동아대학교 의과대학 임상병리학과학교실
Correlation Between CD34+ Cells/subpopulations and CFU-GM in Cord Blood
Background: Human umbilical cord blood can be utilized as a potential source of hematopoietic stem cell transplantation, and the numbers of granulocyte-macrophage
colony forming unit(CFU-GM) and CD34+ cells have been used as parameters of engraftment potential. We studied the relationships between CFU-GM and CD34+ cells/CD34+ cell subpopulations to evaluate which parameter is adequate substitute for clonogenic assays in cord blood engraftment potential.
Methods: We determined the numbers of CD34+ cells, and of CD34+ cells co-expressing CD38 or HLA-DR in 24 cord blood samples which were separated by Ficoll-Hypaque to isolate mononuclear cells and analysed by two color flow cytometry(FACSort, Becton Dikcinson, USA) with anti-CD34-FITC, anti-CD38-PE, and anti-HLA-DR-PE(Becton Dickinson, USA). At the same time, CFU-GM assays were performed in methylcellulose media(Stem Cell Technologies INC, Vancouver, BC, Canada) at 4.0×10 5cells/mL.
Results: Our data showed that the frequency of CD34+ cells in cord blood were 0.67±0.37% of the mononuclear cells and CD34+CD38+, CD34+CD38-, CD34+HLA-DR+, and
CD34+HLA-DR- cells represented 0.54±0.27%, 0.04±0.04%, 0.66±0.33%, and 0.05±0.05% of the mononuclear cells, respectively. Correlation coefficient showed that the numbers of CD34+HLA-DR+ cells and CD34+CD38+ cells, as well as CD34+ cells, correlated positively with the numbers of day 14 CFU-GM. Furthermore, day 14 CFU-GM was
more significantly correlated with the proportion of CD34+HLA-DR+ cells(r=0.58, P<0.01) than CD34+ cells(r=0.55, P<0.01) and CD34+CD38+ cells(r=0.50, P<0.05).
Conclusion: CD34+ cells, CD34+HLA-DR+ cells and CD34+CD38+ cells in cord blood were correlated with CFU-GM. Thus, we suggest that CD34+HLA-DR+ cells and
CD34+38+ cells, as well as CD34+ cells, could be also adequate substitute for clonogenic assays in the evaluation of cord blood engraftment potential.
Keywords Cord blood; CD34+ cell; CD34+ cell subpopulations; CFU-GM;
PDF
Standard view
Export citation
Share Article
Korean J Hematol 1997; 32(2): 214-220
Published online June 30, 1997
Copyright © The Korean Society of Hematology.
제대혈에서의 CD34+세포 및 아형과 CFU-GM과의 상관관계
배광렬, 이영호, 박상수, 김은정, 김태홍, 최안홍, 김태겸, 김경희, 한진영, 김정만
동아대학교 의과대학 소아과학교실,
동아대학교 의과대학 임상병리학과학교실
Correlation Between CD34+ Cells/subpopulations and CFU-GM in Cord Blood
Kwang-Lyul Bae, Young, Ho Lee, Sang Soo Park, Eun, Jung Kim, Tae, Hong Kim, Ahn, Hong Choi, Tae, Gyum Kim, Kyung, Hee Kim, Jin, Yeong Han, Jung, Man Kim
Department of Pediatrics, Clinical Pathology, Dong, A University College of Medicine, Pusan, Korea
Abstract
Background: Human umbilical cord blood can be utilized as a potential source of hematopoietic stem cell transplantation, and the numbers of granulocyte-macrophage
colony forming unit(CFU-GM) and CD34+ cells have been used as parameters of engraftment potential. We studied the relationships between CFU-GM and CD34+ cells/CD34+ cell subpopulations to evaluate which parameter is adequate substitute for clonogenic assays in cord blood engraftment potential.
Methods: We determined the numbers of CD34+ cells, and of CD34+ cells co-expressing CD38 or HLA-DR in 24 cord blood samples which were separated by Ficoll-Hypaque to isolate mononuclear cells and analysed by two color flow cytometry(FACSort, Becton Dikcinson, USA) with anti-CD34-FITC, anti-CD38-PE, and anti-HLA-DR-PE(Becton Dickinson, USA). At the same time, CFU-GM assays were performed in methylcellulose media(Stem Cell Technologies INC, Vancouver, BC, Canada) at 4.0×10 5cells/mL.
Results: Our data showed that the frequency of CD34+ cells in cord blood were 0.67±0.37% of the mononuclear cells and CD34+CD38+, CD34+CD38-, CD34+HLA-DR+, and
CD34+HLA-DR- cells represented 0.54±0.27%, 0.04±0.04%, 0.66±0.33%, and 0.05±0.05% of the mononuclear cells, respectively. Correlation coefficient showed that the numbers of CD34+HLA-DR+ cells and CD34+CD38+ cells, as well as CD34+ cells, correlated positively with the numbers of day 14 CFU-GM. Furthermore, day 14 CFU-GM was
more significantly correlated with the proportion of CD34+HLA-DR+ cells(r=0.58, P<0.01) than CD34+ cells(r=0.55, P<0.01) and CD34+CD38+ cells(r=0.50, P<0.05).
Conclusion: CD34+ cells, CD34+HLA-DR+ cells and CD34+CD38+ cells in cord blood were correlated with CFU-GM. Thus, we suggest that CD34+HLA-DR+ cells and
CD34+38+ cells, as well as CD34+ cells, could be also adequate substitute for clonogenic assays in the evaluation of cord blood engraftment potential.
Keywords: Cord blood, CD34+ cell, CD34+ cell subpopulations, CFU-GM,
Previous Article
