Korean J Hematol 2006; 41(4):
Published online December 30, 2006
https://doi.org/10.5045/kjh.2006.41.4.250
© The Korean Society of Hematology
최상림, 이대형, 정대철, 황희승, 정낙균, 조빈, 한치화, 김학기
가톨릭대학교 의과대학 소아과학교실, 내과학교실
Background:
Intravenous injection of mesenchymal and hematopoietic stem cells (MSCs, HSCs) has the disadvantages of low delivery rate to bone marrow and sequestration of cells in the lung and liver. This study was designed to determine whether there is a relationship between the administration route and dosage of stem cells and GVHD and survival.
Methods:
MSCs were retrieved from five subcultured C3H/10T1/2, cell lines from C3H/He mice. HSCs were transplanted by injecting 1×107 of bone marrow mononuclear cells and 5×106of spleen cells from six to eight week old female C3H/He mice into six week old irradiated female BALB/c mice. The groups were divided into intravenous injection (IV) and intra-marrow (IM) injection groups. IV and IM+MSC groups consisted of mice transplanted with the same bone marrow mononuclear cells and SP, IV and IM groups, with the additional co-injection of 1×106MSCs.
Results:
Evaluation of all mice, in both groups, showed no difference in GVHD and survival. However, high dose injection with 1×106 MSCs led to a decreased incidence of GVHD (P<0.05) and improved survival (P<0.01) in both groups.
Conclusion:
The results of this study showed that the positive effects of MSC on GVHD and survival were primarily dependent on the number of injected cells.
Keywords Graft versus host disease, Mesenchymal stem cell, Route, Dosage
Korean J Hematol 2006; 41(4): 250-258
Published online December 30, 2006 https://doi.org/10.5045/kjh.2006.41.4.250
Copyright © The Korean Society of Hematology.
최상림, 이대형, 정대철, 황희승, 정낙균, 조빈, 한치화, 김학기
가톨릭대학교 의과대학 소아과학교실, 내과학교실
Sang rhim Choi, Dae Hyeoung Lee, Dae Chul Jeong, Hui Sung Hwang, Nack Gyun Chung, Bin Cho, Chi Wha Han, Hack Ki Kim.
Departments of Pediatrics, Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
Background:
Intravenous injection of mesenchymal and hematopoietic stem cells (MSCs, HSCs) has the disadvantages of low delivery rate to bone marrow and sequestration of cells in the lung and liver. This study was designed to determine whether there is a relationship between the administration route and dosage of stem cells and GVHD and survival.
Methods:
MSCs were retrieved from five subcultured C3H/10T1/2, cell lines from C3H/He mice. HSCs were transplanted by injecting 1×107 of bone marrow mononuclear cells and 5×106of spleen cells from six to eight week old female C3H/He mice into six week old irradiated female BALB/c mice. The groups were divided into intravenous injection (IV) and intra-marrow (IM) injection groups. IV and IM+MSC groups consisted of mice transplanted with the same bone marrow mononuclear cells and SP, IV and IM groups, with the additional co-injection of 1×106MSCs.
Results:
Evaluation of all mice, in both groups, showed no difference in GVHD and survival. However, high dose injection with 1×106 MSCs led to a decreased incidence of GVHD (P<0.05) and improved survival (P<0.01) in both groups.
Conclusion:
The results of this study showed that the positive effects of MSC on GVHD and survival were primarily dependent on the number of injected cells.
Keywords: Graft versus host disease, Mesenchymal stem cell, Route, Dosage
Myoung Woo Lee, Dae Seong Kim, Keon Hee Yoo, Hye Ryung Kim, In Keun Jang, Ji Hyang Lee, So Yeon Kim, Meong Hi Son, Soo Hyun Lee, Hye Lim Jung, Ki Woong Sung, and Hong Hoe Koo
Blood Res 2013; 48(2): 107-114Ju Young Yoon, Hyery Kim, Hyoung Jin Kang, Kyung Duk Park, Hee Young Shin, and Hyo Seop Ahn
Korean J Hematol 2012; 47(2): 142-145Ji Young Lim, Bo Gyeong Kim, Seol Kyung Moon, Chang Ki Min
Korean J Hematol 2008; 43(4): 219-231