Korean J Hematol 1993; 28(1):
Published online March 31, 1993
© The Korean Society of Hematology
방사선조사가 백혈구제거 적혈구농축액에 미치는 영향
권오헌, 남정현, 김현옥, 서창옥, 김귀언
연세대학교 의과대학 임상병리과학교실,
연세대학교 의과대학 치료방사선과학교실
In Vitro Storage Lesions of Filterd RBC after Irradiation
Background: While the use of leukodepleted blood can prevent the HLA alloimmunization, febrile transfusion reaction, and transfusion-transmitted cytomegalovirus infection, transfusion-associated graft-versus-host disease (TA-GVHD) can be prevented by the use of irradiated blood. We have used the leukodepleted blood followed by irradiation for preventing these complications, but we sometimes have met difficulties supplying the irradiated, leukodepleted blood when the clinicians need the
transfusion. For this reason, storing the irradiated leukodepleted blood was needed.
Methods: We performed seven biochemical tests (ATP, 2,3-DPG, LDH, plasma free hemoglobin, pH, Na+, K+) on the paired filtered packed red cells, irradiated and non-irradiated, at the day of 0, 1, 3, 7, and 35. We compared the results and evaluated the optimal leukodepletion time.
Results: The biochemical test results showed no significant differences between the irradiated and non-irradiated blood during the storage for 3 days. The irradiated blood showed decreased levels of 2,3-DPG and increased levels of plasma serum LDH and K+ compared to the non-irradiated group for long term storage (p<9.05). In addition, the irradiated units prior to leukodepletion showed more changes in the levels of 2,3-DPG and LDH than the leukodepleted units prior to irradiation.
Conclusion: Filtration prior to irradiation is preferble and the irradiated units should be used at least within 3 days after irradiation.
Keywords Irradiation; Filtered RBC; Transfusion-associated GVHD;
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Korean J Hematol 1993; 28(1): 135-141
Published online March 31, 1993
Copyright © The Korean Society of Hematology.
방사선조사가 백혈구제거 적혈구농축액에 미치는 영향
권오헌, 남정현, 김현옥, 서창옥, 김귀언
연세대학교 의과대학 임상병리과학교실,
연세대학교 의과대학 치료방사선과학교실
In Vitro Storage Lesions of Filterd RBC after Irradiation
Oh Hun Kwon, Chung Hyun Nahm, Hyun Ok Kim, Chang Ok Suh, Gwi Eon Kim
Department of Clinical Pathology, Radiation Oncology, Yonsei University College of Medicine, Seoul, Korea
Abstract
Background: While the use of leukodepleted blood can prevent the HLA alloimmunization, febrile transfusion reaction, and transfusion-transmitted cytomegalovirus infection, transfusion-associated graft-versus-host disease (TA-GVHD) can be prevented by the use of irradiated blood. We have used the leukodepleted blood followed by irradiation for preventing these complications, but we sometimes have met difficulties supplying the irradiated, leukodepleted blood when the clinicians need the
transfusion. For this reason, storing the irradiated leukodepleted blood was needed.
Methods: We performed seven biochemical tests (ATP, 2,3-DPG, LDH, plasma free hemoglobin, pH, Na+, K+) on the paired filtered packed red cells, irradiated and non-irradiated, at the day of 0, 1, 3, 7, and 35. We compared the results and evaluated the optimal leukodepletion time.
Results: The biochemical test results showed no significant differences between the irradiated and non-irradiated blood during the storage for 3 days. The irradiated blood showed decreased levels of 2,3-DPG and increased levels of plasma serum LDH and K+ compared to the non-irradiated group for long term storage (p<9.05). In addition, the irradiated units prior to leukodepletion showed more changes in the levels of 2,3-DPG and LDH than the leukodepleted units prior to irradiation.
Conclusion: Filtration prior to irradiation is preferble and the irradiated units should be used at least within 3 days after irradiation.
Keywords: Irradiation, Filtered RBC, Transfusion-associated GVHD,
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