In hematologic malignancies, recurrent chromosomal abnormalities play a significant role in leukemogenesis . Gene fusions and mutations, including those in
In this study, we analyzed the characteristics and clinical significance of
This study was approved by the institutional review board of the Seoul St. Mary's Hospital, the Catholic University of Korea (KC17SESE0768). A total of 142 adult patients, recently diagnosed with de novo AML at the Seoul St. Mary's Hospital from June 2015–February 2017, were enrolled in the study, consecutively. Patients who had undergone chemotherapy previously or had an antecedent hematologic disease, acute promyelocytic leukemia with
Bone marrow cells were aspirated from patients, cultured under unstimulated culture conditions for 1 d–2 d, and harvested. Karyotyping was carried out using the Giemsa banding techniques. At least 20 metaphase-cells were analyzed. Cytogenetic abnormalities were classified according to the 2016 guidelines of the International System for Human Cytogenetic Nomenclature . Mutations in
CpG methylation in the promoter region of
Primers and probes were derived from RefSeq NM_022552.4 using the OLIGO ver. 7.51 software (Molecular Biology Insights, Inc., Cascade, CO, USA). The PCR reaction was carried out as follows: 2 min at 50℃, 10 min at 95℃, 50 cycles of 15 s at 95℃, and 1 min at 60℃ on a Mx3000PTM Real-Time PCR system (Stratagene, San Diego, CA, USA). Data were analyzed using the MxPro version 4.10 (Stratagene).
The correlations between parameters compared in contingency tables were found using the Chi-squared (χ2) test. The Kaplan-Meier survival analysis was used to estimate overall survival (OS) and to compare differences between survival curves. OS was measured from date of initial diagnosis to the date of death, or last follow-up, from any cause. The multivariate Cox proportional-hazards regression method was used to analyze independent prognostic factors for OS. The variables, including age; karyotype; presence of mutations in
Among the 142 patients enrolled with de novo AML, 82 were males and 60 were females. The median age was 53.5 years (range, 17–88 yr).
We identified 19 different
We measured the value of
We also measured
We performed multivariate analysis with variables that included the
We performed quantitative analysis of the R882H mutation in
Previously, a strong correlation between decreased DNA methylation and
Moreover, the presence of
In addition, we observed that
We demonstrated that the
This study was supported by a grant from the Korea Health Technology R&D Project, the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant no. HI18C0480) and the research fund of the Seoul St. Mary's Hospital, The Catholic University of Korea.
No potential conflicts of interest relevant to this article were reported.