Blood Res 2019; 54(4):
Published online December 31, 2019
https://doi.org/10.5045/br.2019.54.4.274
© The Korean Society of Hematology
Correspondence to : Neveen Lewis Mikhael, M.D.
Clinical Pathology Department, Alexandria Faculty of Medicine, Elkhartoum Square, Alexandria, Egypt
E-mail: Neveen.Lewis@alexmed.edu.eg
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Hematopoietic stem cell transplantation (HSCT) is a well-established treatment modality for a variety of diseases. Immune reconstitution is an important event that determines outcomes. The immune recovery of T cells relies on peripheral expansion of mature graft cells, followed by differentiation of donor-derived hematopoietic stem cells. The formation of new T cells occurs in the thymus and as a byproduct, T cell receptor excision circles (TRECs) are released. Detection of TRECs by PCR is a reliable method for estimating the amount of newly formed T cells in the circulation and, indirectly, for estimating thymic function. The aim of this study was to determine the role of TREC quantitation in predicting outcomes of human leucocyte antigen (HLA) identical allogenic HSCT.
The study was conducted on 100 patients receiving allogenic HSCT from an HLA identical sibling. TREC quantification was done by real time PCR using a standard curve.
TREC levels were inversely related to age (
Age and nature of disease determine the TREC levels, which are related to relapse.
Keywords TRECs, Immune, Allogenic, HSCT, Outcomes
Blood Res 2019; 54(4): 274-281
Published online December 31, 2019 https://doi.org/10.5045/br.2019.54.4.274
Copyright © The Korean Society of Hematology.
Neveen Lewis Mikhael1, Manal Elsorady2
1Clinical Pathology Department, Alexandria Faculty of Medicine, 2Clinical Hematology Department, Head of BMT Unit, Alexandria Faculty of Medicine, Alexandria, Egypt
Correspondence to:Neveen Lewis Mikhael, M.D.
Clinical Pathology Department, Alexandria Faculty of Medicine, Elkhartoum Square, Alexandria, Egypt
E-mail: Neveen.Lewis@alexmed.edu.eg
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Hematopoietic stem cell transplantation (HSCT) is a well-established treatment modality for a variety of diseases. Immune reconstitution is an important event that determines outcomes. The immune recovery of T cells relies on peripheral expansion of mature graft cells, followed by differentiation of donor-derived hematopoietic stem cells. The formation of new T cells occurs in the thymus and as a byproduct, T cell receptor excision circles (TRECs) are released. Detection of TRECs by PCR is a reliable method for estimating the amount of newly formed T cells in the circulation and, indirectly, for estimating thymic function. The aim of this study was to determine the role of TREC quantitation in predicting outcomes of human leucocyte antigen (HLA) identical allogenic HSCT.
The study was conducted on 100 patients receiving allogenic HSCT from an HLA identical sibling. TREC quantification was done by real time PCR using a standard curve.
TREC levels were inversely related to age (
Age and nature of disease determine the TREC levels, which are related to relapse.
Keywords: TRECs, Immune, Allogenic, HSCT, Outcomes
TREC standard curve.
Correlation between TRECs and age in patients and controls.
Comparison between TREC levels at day 28 in patients with benign and malignant disease.
TREC levels at day 28 and outcomes of transplantation.
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TREC standard curve.
|@|~(^,^)~|@|Correlation between TRECs and age in patients and controls.
|@|~(^,^)~|@|Comparison between TREC levels at day 28 in patients with benign and malignant disease.
|@|~(^,^)~|@|TREC levels at day 28 and outcomes of transplantation.