Interleukin-1B (IL-1B-31 and IL-1B-511) and interleukin-1 receptor antagonist (IL-1Ra) gene polymorphisms in primary immune thrombocytopenia

Deependra Kumar Yadav; Anil Kumar Tripathi; Divya Gupta; Saurabh Shukla; Aloukick Kumar Singh; Ashutosh Kumar; Jyotsna Agarwal; K. N. Prasad

doi:10.5045/br.2017.52.4.264

Quick links

Most Cited Most Read Ahead of Print Archives Go to E-submission

Original Article

Split Viewer

Blood Res 2017; 52(4):

Published online December 31, 2017

https://doi.org/10.5045/br.2017.52.4.264

Interleukin-1B (IL-1B-31 and IL-1B-511) and interleukin-1 receptor antagonist (IL-1Ra) gene polymorphisms in primary immune thrombocytopenia

Deependra Kumar Yadav¹, Anil Kumar Tripathi^1*, Divya Gupta², Saurabh Shukla¹, Aloukick Kumar Singh⁵, Ashutosh Kumar³, Jyotsna Agarwal⁴, and K. N. Prasad⁵

Author Info. +

¹Department of Clinical Hematology, King George's Medical University, Lucknow, India.

²Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.

³Department of Pathology, King George's Medical University, Lucknow, India.

⁴Department of Microbiology, King George's Medical University, Lucknow, India.

⁵Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.

Correspondence to : Anil Kumar Tripathi, M.D. Department of Clinical Hematology, King George's Medical University, Lucknow - 226003, India. aktkgmu@gmail.com

Received: December 6, 2016; Revised: May 19, 2017; Accepted: June 29, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Go to

Abstract

Background

Immune thrombocytopenia (ITP) is an immune-mediated disease caused by autoantibodies against platelets membrane glycoproteins GPIIb/IIIa and GPIb/IX. The etiology of ITP remains unclear. This study evaluated the association of polymorphisms in interleukin (IL)-1B-31, IL-1B-511, and IL-1Ra with ITP.

Methods

Genotyping of IL-1B-31, IL-1B-511, and IL-1Ra was performed in 118 ITP patients and 100 controls by polymerase chain reaction restriction fragment length polymorphism and detection of variable number tandem repeats.

Results

Genotype differences in IL-1B-31 and IL-1Ra were significantly associated with ITP. Patients showed a higher frequency of the IL-1B-31 variant allele (T) and a 1.52-fold greater risk of susceptibility to ITP (odds ratio [OR]=1.52, 95% confidence interval [CI]=1.04?2.22, P=0.034). The frequencies of both homozygous and heterozygous variant genotypes of IL-1B-31 were higher (OR=2.33, 95% CI=1.069?5.09, P=0.033 and OR=2.044, 95% CI=1.068?39, P=0.034) among patients and were significantly associated with ITP susceptibility. Both homozygous and heterozygous variant genotypes of IL-1Ra were also more frequent (OR=4.48, 95% CI=1.17?17.05, P=0.0230 and OR=1.80, 95% CI=1.03?3.14, P=0.0494) among patients and were associated with ITP risk. IL-1B-31 and IL-1Ra also showed significant association with severe ITP. However, IL-1B-511 was not associated with ITP.

Conclusion

IL-1B-31 and IL-1Ra polymorphisms may significantly impact ITP risk, and they could be associated with disease severity, which may contribute to the pathogenesis of ITP.

Keywords Primary immune thrombocytopenia, IL-1B-31, IL-1B-511, IL-1Ra polymorphism, PCR-RFLP

Article

Original Article

Blood Res 2017; 52(4): 264-269

Published online December 31, 2017 https://doi.org/10.5045/br.2017.52.4.264

Interleukin-1B (IL-1B-31 and IL-1B-511) and interleukin-1 receptor antagonist (IL-1Ra) gene polymorphisms in primary immune thrombocytopenia

Deependra Kumar Yadav¹, Anil Kumar Tripathi^1*, Divya Gupta², Saurabh Shukla¹, Aloukick Kumar Singh⁵, Ashutosh Kumar³, Jyotsna Agarwal⁴, and K. N. Prasad⁵

¹Department of Clinical Hematology, King George's Medical University, Lucknow, India.

²Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.

³Department of Pathology, King George's Medical University, Lucknow, India.

⁴Department of Microbiology, King George's Medical University, Lucknow, India.

⁵Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.

Correspondence to:Anil Kumar Tripathi, M.D. Department of Clinical Hematology, King George's Medical University, Lucknow - 226003, India. aktkgmu@gmail.com

Received: December 6, 2016; Revised: May 19, 2017; Accepted: June 29, 2017

Abstract

Background

Methods

Results

Conclusion

IL-1B-31 and IL-1Ra polymorphisms may significantly impact ITP risk, and they could be associated with disease severity, which may contribute to the pathogenesis of ITP.

Keywords: Primary immune thrombocytopenia, IL-1B-31, IL-1B-511, IL-1Ra polymorphism, PCR-RFLP

Abstract

Table 1 Demographic details of ITP patients and healthy control subjects.

Table 2 Influence of <italic>IL-1B-31</italic>, <italic>IL-1B-511</italic>, and <italic>IL-1Ra</italic> polymorphisms on susceptibility to ITP and on control subjects.

^a)Statistically significant susceptible genotype..

Abbreviations: CI, confidence interval; OR, odds ratio..

Table 3 Influence of <italic>IL-1B-31</italic>, <italic>IL-1B-511</italic> and <italic>IL-1Ra</italic> polymorphisms on severe, non-severe ITP patients and control subjects.

^a)Statistically significant susceptible genotype..

Abbreviations: CI; confidence Interval; OR, odds ratio..

Table 4 <italic>IL-1B-31</italic>, <italic>IL-1B-511</italic>, and <italic>IL-1Ra</italic> genotype distribution in responder (complete+partial responder) and non-responder.

^a)Statistically significant susceptible genotype..

Abbreviations: CI, confidence interval; OR, odds ratio..