Blood Res 2015; 50(2):
Published online June 25, 2015
https://doi.org/10.5045/br.2015.50.2.80
© The Korean Society of Hematology
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
2Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Correspondence to : Correspondence to Mehryar Habibi Roudkenar, Ph.D. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization (IBTO), Hemmat Exp. Way, 14665-1157, Tehran, Iran. Tel: +982188613423, Fax: +982188601555, roudkenar@ibto.ir
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Mesenchymal stem cells (MSCs) are valuable for cell-based therapy. However, their application is limited owing to their low survival rate when exposed to stressful conditions. Autophagy, the process by which cells recycle the cytoplasm and dispose of defective organelles, is activated by stress stimuli to adapt, tolerate adverse conditions, or trigger the apoptotic machinery. This study aimed to determine whether regulation of autophagy would affect the survival of MSCs under stress conditions.
Autophagy was induced in bone marrow-derived MSCs (BM-MSCs) by rapamycin, and was inhibited via shRNA-mediated knockdown of the autophagy specific gene,
Of 4 specific
Autophagy modulation in MSCs can be proposed as a new strategy to improve their survival rate in stressful microenvironments.
Keywords Autophagy, Mesenchymal stem cells, Oxidative stress, Cell survival, shRNA
Blood Res 2015; 50(2): 80-86
Published online June 25, 2015 https://doi.org/10.5045/br.2015.50.2.80
Copyright © The Korean Society of Hematology.
Sedigheh Molaei1, Mehryar Habibi Roudkenar1*, Fatemeh Amiri1, Mozhgan Dehghan Harati1, Marzie Bahadori1, Fatemeh Jaleh1, Mohammad Ali Jalili1, and Amaneh Mohammadi Roushandeh2
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
2Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Correspondence to: Correspondence to Mehryar Habibi Roudkenar, Ph.D. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization (IBTO), Hemmat Exp. Way, 14665-1157, Tehran, Iran. Tel: +982188613423, Fax: +982188601555, roudkenar@ibto.ir
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Mesenchymal stem cells (MSCs) are valuable for cell-based therapy. However, their application is limited owing to their low survival rate when exposed to stressful conditions. Autophagy, the process by which cells recycle the cytoplasm and dispose of defective organelles, is activated by stress stimuli to adapt, tolerate adverse conditions, or trigger the apoptotic machinery. This study aimed to determine whether regulation of autophagy would affect the survival of MSCs under stress conditions.
Autophagy was induced in bone marrow-derived MSCs (BM-MSCs) by rapamycin, and was inhibited via shRNA-mediated knockdown of the autophagy specific gene,
Of 4 specific
Autophagy modulation in MSCs can be proposed as a new strategy to improve their survival rate in stressful microenvironments.
Keywords: Autophagy, Mesenchymal stem cells, Oxidative stress, Cell survival, shRNA
Confirmation of autophagy suppression via
Confirmation of autophagy induction with rapamycin.
WST-1 assay to detect the effects of autophagy on hBM-MSC survival.
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Confirmation of autophagy suppression via
Confirmation of autophagy induction with rapamycin.
WST-1 assay to detect the effects of autophagy on hBM-MSC survival.