Original Article
Korean J Hematol 2006; 41(3):
Published online September 30, 2006
https://doi.org/10.5045/kjh.2006.41.3.167
© The Korean Society of Hematology
비예기항체 동정검사 시 효소법 추가 검사의 진단적 유용성
이대동, 오승환, 신호진, 정주섭, 조군제, 박태성, 이은엽, 손한철, 김형회
부산대학교 의학전문대학원 진단검사의학교실, 내과학교실,
국군수도병원 진단검사의학과
Diagnostic Usefulness of Antibody Identification Test Including Enzyme Method
Background:
Many medical institutions in Korea have recently been performing an antibody screening test as one of the essential elements of a pre-transfusion test. In this study we will determine the advantage and clinical significance of adding an enzyme method to the antiglobulin method while conducting the antibody identification test.
Methods:
We performed antibody identification tests between December 2002 and December 2005, for a total of 37 months at Pusan National University Hospital. In this study we have analyzed 550 cases that were conducted by both the antiglobulin method and the enzyme method at the same time.
Results:
A total of 111 of the results were cases of detection using the adding an enzyme method. Among these results, Rh antibodies that included the anti-E had the highest number of results 77 (69.4%), 28 antibody (25.2%), 2 anti-P1 (1.8%) and one anti-Jkb(0.9%).
Conclusion:
Using the enzyme method in the antibody identification test proved to us that there were more clinically significant warm antibodies than cold antibodies. In order to have a more secured transfusion, it is required to identify a clinically significant antibody using the additional enzyme method during the antibody identification test.
Keywords Antibody identification test, Antiglobulin method, Enzyme method
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Original Article
Korean J Hematol 2006; 41(3): 167-171
Published online September 30, 2006 https://doi.org/10.5045/kjh.2006.41.3.167
Copyright © The Korean Society of Hematology.
비예기항체 동정검사 시 효소법 추가 검사의 진단적 유용성
이대동, 오승환, 신호진, 정주섭, 조군제, 박태성, 이은엽, 손한철, 김형회
부산대학교 의학전문대학원 진단검사의학교실, 내과학교실,
국군수도병원 진단검사의학과
Diagnostic Usefulness of Antibody Identification Test Including Enzyme Method
Dae Dong Lee, Seung Hwan Oh, Ho Jin Shin, Joo Seop Chung, Goon Jae Cho, Tae Sung Park, Eun Yup Lee, Han Chul Son, Hyung Hoi Kim
Departments of Laboratory Medicine and, Internal Medicine, Pusan National University School of Medicine, Busan,
Department of Laboratory Medicine, Armed Forces Capital Hospital, Seongnam, Korea
Abstract
Background:
Many medical institutions in Korea have recently been performing an antibody screening test as one of the essential elements of a pre-transfusion test. In this study we will determine the advantage and clinical significance of adding an enzyme method to the antiglobulin method while conducting the antibody identification test.
Methods:
We performed antibody identification tests between December 2002 and December 2005, for a total of 37 months at Pusan National University Hospital. In this study we have analyzed 550 cases that were conducted by both the antiglobulin method and the enzyme method at the same time.
Results:
A total of 111 of the results were cases of detection using the adding an enzyme method. Among these results, Rh antibodies that included the anti-E had the highest number of results 77 (69.4%), 28 antibody (25.2%), 2 anti-P1 (1.8%) and one anti-Jkb(0.9%).
Conclusion:
Using the enzyme method in the antibody identification test proved to us that there were more clinically significant warm antibodies than cold antibodies. In order to have a more secured transfusion, it is required to identify a clinically significant antibody using the additional enzyme method during the antibody identification test.
Keywords: Antibody identification test, Antiglobulin method, Enzyme method
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