Korean J Hematol 2009; 44(4):
Published online December 30, 2009
https://doi.org/10.5045/kjh.2009.44.4.199
© The Korean Society of Hematology
신가희 박경순 정광회 이태희
차의과학대학교 의생명과학부,
연세대학교 자연과학연구소 종양줄기세포연구실
Background: Vascular endothelial growth factor (VEGF) plays an essential role in promoting angiogenesis during tumor development. In addition, VEGF can mediate the inflammatory response in tumors. VEGF increases the level of neutrophil migration by upregulating interleukin-8 (IL-8) in endothelial cells in vitro. However, it is unclear if VEGF can mediate IL-8 production in vivo.
Methods: To address this issue, this study examined the effect of VEGF on IL-8 production in vivo using an adenovirus transduction and mouse ear assay.
Results: Adenovirus-encoded VEGF (VEGF-Ad) increased the level of IL-8 production in endothelial cells in vitro compared to the control-adenovirus (CTL-Ad). The mouse ear assay showed that VEGF-Ad increased the level of IL-8 production in the endothelium. Immunohistochemistry showed that the IL-8 proteins were expressed in the vasculature within a human glioblastoma, which is known to strongly express VEGF.
Conclusion: These results suggest that VEGF can mediate the inflammatory response in endothelial cells in vivo via the up-regulation of IL-8. (Korean J Hematol 2009;44:199-204.)
Keywords Vascular endothelial growth factor, Interleukin-8, Endothelial cells, Chemokine, Inflammation
Korean J Hematol 2009; 44(4): 199-204
Published online December 30, 2009 https://doi.org/10.5045/kjh.2009.44.4.199
Copyright © The Korean Society of Hematology.
신가희 박경순 정광회 이태희
차의과학대학교 의생명과학부,
연세대학교 자연과학연구소 종양줄기세포연구실
Ga Hee Shin, Kyung Soon Park, Kwang Hoe Chung, Tae Hee Lee
Thrombosis and Vascular Biology Lab., Department of Medical Science, CHA University, Seongnam
Laboratoryof Cancer Stem Cell Biology, Natural Science Institute, Yonsei University, Seoul, Korea
Background: Vascular endothelial growth factor (VEGF) plays an essential role in promoting angiogenesis during tumor development. In addition, VEGF can mediate the inflammatory response in tumors. VEGF increases the level of neutrophil migration by upregulating interleukin-8 (IL-8) in endothelial cells in vitro. However, it is unclear if VEGF can mediate IL-8 production in vivo.
Methods: To address this issue, this study examined the effect of VEGF on IL-8 production in vivo using an adenovirus transduction and mouse ear assay.
Results: Adenovirus-encoded VEGF (VEGF-Ad) increased the level of IL-8 production in endothelial cells in vitro compared to the control-adenovirus (CTL-Ad). The mouse ear assay showed that VEGF-Ad increased the level of IL-8 production in the endothelium. Immunohistochemistry showed that the IL-8 proteins were expressed in the vasculature within a human glioblastoma, which is known to strongly express VEGF.
Conclusion: These results suggest that VEGF can mediate the inflammatory response in endothelial cells in vivo via the up-regulation of IL-8. (Korean J Hematol 2009;44:199-204.)
Keywords: Vascular endothelial growth factor, Interleukin-8, Endothelial cells, Chemokine, Inflammation
Yu Ri Kim
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