Korean J Hematol 2007; 42(3):
Published online September 30, 2007
https://doi.org/10.5045/kjh.2007.42.3.224
© The Korean Society of Hematology
김동훈, 김무정, 조진희, 임남규, 박정일, 정성현, 이현우, 강석윤, 최진혁, 김효철, 박준성
아주대학교 의과대학 종양혈액내과학교실
Background:
It is still obscure how dendritic cells (DCs) can orchestrate whole immune reactions according to the host age. We studied changes of murine splenic DCs after total body irradiation (TBI), with regards to age.
Methods:
Young (8∼14 wk) and old (12∼16 mo) C57Bl/6 mice were irradiated with a dose of 1,100 cGy and were assessed 6 h later for phenotypic and functional changes of the DCs. The mean fluorescence intensities and cytokine producing cell proportions were analyzed with the student’s t-test.
Results:
Interleukin-12 (IL-12), interferon (IFNγ) and tumor necrosis factor (TNFα) producing classical DCs (cDCs) were more numerous in the young untreated mice than in the old mice. However, the number of these cells decreased in the young mice and increased in the old mice after TBI. IL-12, IFNγ and TNFα producing plasmacytoid DCs (pDCs) were more frequent in the old mice than in the young mice before TBI both mice showed an increased frequency of cells producing these cytokines after TBI. Overall, the highest numbers of cDCs and pDCs producing IL-12, IFNγ and TNFα were present in the old mice after TBI. In both the cDC and pDC populations, the old mice had a higher frequency of IL-10+ cells prior to TBI. After irradiation, the young mice had a higher frequency of IL-10+ cells.
Conclusion:
With TBI, the DCs showed dramatic differences between young and old mice. Young mice turned to an immuno-suppressive response whereas the old mice changed to an immuno-stimulation of DCs after TBI. From these dramatic aging effects, we hope to explain the different frequencies and severities of acute GvHD after allogeneic hematopoietic stem cell transplantation according to host age.
Keywords Dendritic cells, Age, Total body irradiation
Korean J Hematol 2007; 42(3): 224-232
Published online September 30, 2007 https://doi.org/10.5045/kjh.2007.42.3.224
Copyright © The Korean Society of Hematology.
김동훈, 김무정, 조진희, 임남규, 박정일, 정성현, 이현우, 강석윤, 최진혁, 김효철, 박준성
아주대학교 의과대학 종양혈액내과학교실
Dong Hoon Kim, Moo Jung Kim, Jin Hee Cho, Nam Kyu Lim, Jung Il Park, Seong Hyun Jeong, Hyun Woo Lee, Seok Yun Kang, Jin Hyuk Choi, Hugh Chul Kim, Joon Seong Park
Department of Hematology, Oncology, Ajou University School of Medicine, Suwon, Korea
Background:
It is still obscure how dendritic cells (DCs) can orchestrate whole immune reactions according to the host age. We studied changes of murine splenic DCs after total body irradiation (TBI), with regards to age.
Methods:
Young (8∼14 wk) and old (12∼16 mo) C57Bl/6 mice were irradiated with a dose of 1,100 cGy and were assessed 6 h later for phenotypic and functional changes of the DCs. The mean fluorescence intensities and cytokine producing cell proportions were analyzed with the student’s t-test.
Results:
Interleukin-12 (IL-12), interferon (IFNγ) and tumor necrosis factor (TNFα) producing classical DCs (cDCs) were more numerous in the young untreated mice than in the old mice. However, the number of these cells decreased in the young mice and increased in the old mice after TBI. IL-12, IFNγ and TNFα producing plasmacytoid DCs (pDCs) were more frequent in the old mice than in the young mice before TBI both mice showed an increased frequency of cells producing these cytokines after TBI. Overall, the highest numbers of cDCs and pDCs producing IL-12, IFNγ and TNFα were present in the old mice after TBI. In both the cDC and pDC populations, the old mice had a higher frequency of IL-10+ cells prior to TBI. After irradiation, the young mice had a higher frequency of IL-10+ cells.
Conclusion:
With TBI, the DCs showed dramatic differences between young and old mice. Young mice turned to an immuno-suppressive response whereas the old mice changed to an immuno-stimulation of DCs after TBI. From these dramatic aging effects, we hope to explain the different frequencies and severities of acute GvHD after allogeneic hematopoietic stem cell transplantation according to host age.
Keywords: Dendritic cells, Age, Total body irradiation
Wonjin Jang, Suejung Jo, Jae Won Yoo, Seongkoo Kim, Jae Wook Lee, Pil-Sang Jang, Nack-Gyun Chung, Bin Cho
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