Korean J Hematol 1993; 28(2):
Published online June 30, 1993
© The Korean Society of Hematology
급성 백혈병세포에서 c-kit 표현에 관한 연구(I)
민유홍, 장길진, 라선영, 이선주, 한지숙, 고윤웅
연세대학교 의과대학 내과학교실
Analysis of c-kit Expression in Acute Leukemic Cells(I)
Background: The Proto-oncogene c-kit encodes a transmembrane receptor tyrosine kinase, the ligand of which was recently cloned and identified as the stem cell factor
(SCF). It has been suggested that c-kit-SCF axis is important in the regulation of ""hematopoiesis. It was shown that a significant percentage of acute myeloid, but not
lymphoid, leukemias express the c-kit receptor. Therefore the role of the c-kit in acute leukemia has to be elucidated. We analyzed the c-kit expression and CD34 coexpression of acute leukemic blasts as a first step to evaluating the role of c-kit-SCF axis in acute myeloid leukemia.
Material and Methods:
Bone marrow mononuclear cell specimens, obtained from 33 patients with newly-diagnosed acute leukemia(AML 18 cases, ALL 15 cases) were
assayed for the presence of c-kit cell-surface antigen identified by monoclonal antibody 17F11. The expression of c-kit was studied by means of indirect immunofluorescence
cytometry.
Results:
c-kit was expressed in 6 cases with AML(6/18, 33.3%), but was absent in all patients with ALL(0/l5), and in normal bone marrow donors(0/7). 17F11-reactive AML
samples contained a subpopulation expressing c-kit between 5.7% to 58.2%. It was shown that 19.2-92.9% of c=kit+ cells coexpress CD34 antigen.
Conclusion:
These results indicat that c-kit is expressed in cases of AML. Functional characterization and molecuar identification of c-kit product in AML cells will not only
provide important insights into fundamental mechanisms underlying regulation of normal hematopoiesis, but also provide further understanding of abnormal growth of AML cells.
Keywords Acute leukemia; c-kit;
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Korean J Hematol 1993; 28(2): 267-275
Published online June 30, 1993
Copyright © The Korean Society of Hematology.
급성 백혈병세포에서 c-kit 표현에 관한 연구(I)
민유홍, 장길진, 라선영, 이선주, 한지숙, 고윤웅
연세대학교 의과대학 내과학교실
Analysis of c-kit Expression in Acute Leukemic Cells(I)
Yoo Hong Min, Gil Jin Jang, Sun Yung Ra, Sun Ju Lee, Jee Sook Hahn, Yun Woong Ko
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
Abstract
Background: The Proto-oncogene c-kit encodes a transmembrane receptor tyrosine kinase, the ligand of which was recently cloned and identified as the stem cell factor
(SCF). It has been suggested that c-kit-SCF axis is important in the regulation of ""hematopoiesis. It was shown that a significant percentage of acute myeloid, but not
lymphoid, leukemias express the c-kit receptor. Therefore the role of the c-kit in acute leukemia has to be elucidated. We analyzed the c-kit expression and CD34 coexpression of acute leukemic blasts as a first step to evaluating the role of c-kit-SCF axis in acute myeloid leukemia.
Material and Methods:
Bone marrow mononuclear cell specimens, obtained from 33 patients with newly-diagnosed acute leukemia(AML 18 cases, ALL 15 cases) were
assayed for the presence of c-kit cell-surface antigen identified by monoclonal antibody 17F11. The expression of c-kit was studied by means of indirect immunofluorescence
cytometry.
Results:
c-kit was expressed in 6 cases with AML(6/18, 33.3%), but was absent in all patients with ALL(0/l5), and in normal bone marrow donors(0/7). 17F11-reactive AML
samples contained a subpopulation expressing c-kit between 5.7% to 58.2%. It was shown that 19.2-92.9% of c=kit+ cells coexpress CD34 antigen.
Conclusion:
These results indicat that c-kit is expressed in cases of AML. Functional characterization and molecuar identification of c-kit product in AML cells will not only
provide important insights into fundamental mechanisms underlying regulation of normal hematopoiesis, but also provide further understanding of abnormal growth of AML cells.
Keywords: Acute leukemia, c-kit,
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