Korean J Hematol 1993; 28(1):
Published online March 31, 1993
© The Korean Society of Hematology
급성백혈병에서 화학요법후 골수기능 회복시 말초혈액내 CD34+세포 출현에 관한 연구
-말초혈액 조혈모세포이식을 위한 예비연구 (I)-
민유홍, 최동훈, 이승태, 남동기, 이선주, 한지숙, 고윤웅
연세대학교 의과대학 내과학교실
A study on the Circulation of CD34+ Cells in the Peripherar Blood of Chemotherapy-Treated Patients with Acute Leukemia during Bone Marrow Recovery
- Preliminary Study for Peripheral Blood Stem Cell Transplantation (I) -
Background: The study of peripheral blood hemopoietic stem cells (PHSC) involved in hematopoietic reconstitution after PHSC transplantation has been hampered by their low
frequency and by the limitations of clonal culture technique. Virtually all hematopoietic progenitor cells express the CD34-defined antigen and CD34+ cells
circulate in the peripheral blood during the hematopoietic recovery that follows pancytopenia by chemotherapy. This study is to investigate the mobilization of
CD34+ cells in PB during marrow recovery after chemotherapy in patients with acute leukemia.
Method : Peripheral blood levels of CD34+ cells were studied serially in 14 cases with acute leukemia in remission during the period of recovery that followed
induction or consolidation chemotherapy using direct immunofluorescence flow cytometry assay.
Results: CD34+ cells were detectable in PB from 25.5 days(AML), and 23.5 days (ALL) after initiation of chemotherapy. The medain duration of PB CD34+ cells mobilization was 8 days in AML, and 5.5 days in ALL
respectively. Median peak levels of PB CD34+ cells tend to be higher in ALL (216.8/μl) than in AML (101.8/μl), and the highest levels were observed in
patients with ALL undergoing first induction chemotherapy. There was no difference of the median number of days from the initiation of chemotherapy to the peak levels of
CD34+ cells between cases with AML (27 days) and ALL(26.5 days). In rhGM-CSF-treated patients with AML, there seemed to show somewhat higher absolute
granulocyte counts and lesser platelets on the date of peak CD34+ cells compared to the untreated patients.
Conclusion: We show that there were overshoots of circulating CD34+ cells to a variable degree in patients with acute leukemia recovering from
chemotherapy-induced pancytopenia. Real-time evaluation of the CD34+ cells in the peripheral blood might be a guide to harvest circulating hematopoietic progenitors for autologous PHSCT.
Keywords Acute leukemia; Peripheral blood; CD34+ cells;
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Korean J Hematol 1993; 28(1): 55-69
Published online March 31, 1993
Copyright © The Korean Society of Hematology.
급성백혈병에서 화학요법후 골수기능 회복시 말초혈액내 CD34+세포 출현에 관한 연구
-말초혈액 조혈모세포이식을 위한 예비연구 (I)-
민유홍, 최동훈, 이승태, 남동기, 이선주, 한지숙, 고윤웅
연세대학교 의과대학 내과학교실
A study on the Circulation of CD34+ Cells in the Peripherar Blood of Chemotherapy-Treated Patients with Acute Leukemia during Bone Marrow Recovery
- Preliminary Study for Peripheral Blood Stem Cell Transplantation (I) -
Yoo Hong Min, Dong Hun Choi, Seung Tae Lee, Dong Ki Nam, Sun Ju Lee, Jee Sook Hahn, Yun Woong Ko
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
Abstract
Background: The study of peripheral blood hemopoietic stem cells (PHSC) involved in hematopoietic reconstitution after PHSC transplantation has been hampered by their low
frequency and by the limitations of clonal culture technique. Virtually all hematopoietic progenitor cells express the CD34-defined antigen and CD34+ cells
circulate in the peripheral blood during the hematopoietic recovery that follows pancytopenia by chemotherapy. This study is to investigate the mobilization of
CD34+ cells in PB during marrow recovery after chemotherapy in patients with acute leukemia.
Method : Peripheral blood levels of CD34+ cells were studied serially in 14 cases with acute leukemia in remission during the period of recovery that followed
induction or consolidation chemotherapy using direct immunofluorescence flow cytometry assay.
Results: CD34+ cells were detectable in PB from 25.5 days(AML), and 23.5 days (ALL) after initiation of chemotherapy. The medain duration of PB CD34+ cells mobilization was 8 days in AML, and 5.5 days in ALL
respectively. Median peak levels of PB CD34+ cells tend to be higher in ALL (216.8/μl) than in AML (101.8/μl), and the highest levels were observed in
patients with ALL undergoing first induction chemotherapy. There was no difference of the median number of days from the initiation of chemotherapy to the peak levels of
CD34+ cells between cases with AML (27 days) and ALL(26.5 days). In rhGM-CSF-treated patients with AML, there seemed to show somewhat higher absolute
granulocyte counts and lesser platelets on the date of peak CD34+ cells compared to the untreated patients.
Conclusion: We show that there were overshoots of circulating CD34+ cells to a variable degree in patients with acute leukemia recovering from
chemotherapy-induced pancytopenia. Real-time evaluation of the CD34+ cells in the peripheral blood might be a guide to harvest circulating hematopoietic progenitors for autologous PHSCT.
Keywords: Acute leukemia, Peripheral blood, CD34+ cells,
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