Korean J Hematol 2008; 43(3):
Published online September 30, 2008
https://doi.org/10.5045/kjh.2008.43.3.150
© The Korean Society of Hematology
정영숙 배이영 정낙균 조빈 김학기 민창기 한치화 김호식 정대철
가톨릭대학교 의과대학 소아과학교실, 내과학교실, 생화학교실
Background: The iron chelating agents (ICA) have various biological effects besides iron chelation. We investigated the immunomodulatory effects of Deferasirox (DFS) compared to Deferoxamine (DFO). Methods: Spleen cells (SP) were obtained from 5 week-old C57/BL6 (H-2b). The cytotoxicity of ICAs was examined using the CCK8 method. For the cell proliferation assay, SP were cultured with irradiated in addition to 10, 50, 100ՌM of DFS or DFO and 200ng/mL of cyclosporin A (CSA). Cytokines and nitrite levels were evaluated from supernatants by ELISA. Results: The viability of ICA was reported to be over 100%. Both DFS and DFO inhibited cell proliferation in a manner comparable to CSA. Cell proliferation without iron was reduced at the concentration of 100ՌM of DFO. With iron treatment, the reduction of the stimulation index was dependent on DFO concentrations. DFS decreased the proliferation without reference to the concentrations. After stimulation of phytohemagglutinin, the nitrite concentrations increased with iron. With lipopolysaccharides, the nitrite levels were higher in DFO with iron than control, but similar in DFS regardless of iron treatment. The levels of interleukin-2 were not different. Interleukin-10 was more abundantly produced in 50ՌM of DFO compared to DFS. Transforming growth factor-Ղ was higher in DFS than DFO at the low concentration, but opposite at the high concentration. Conclusion: These data suggested that both iron chelating agents possessed immune suppressive effects comparable to CSA. The immunosuppressive effect of DFS may be distinct from DFO. More experiments are required to determine the exact mechanism of the immunosuppressive effect of DFS. (Korean J Hematol 2008;43:150-158.)
Keywords Deferoxamine, Deferasirox, Immunosuppressive effect
Korean J Hematol 2008; 43(3): 150-158
Published online September 30, 2008 https://doi.org/10.5045/kjh.2008.43.3.150
Copyright © The Korean Society of Hematology.
정영숙 배이영 정낙균 조빈 김학기 민창기 한치화 김호식 정대철
가톨릭대학교 의과대학 소아과학교실, 내과학교실, 생화학교실
Yeong Suk Jung, E Young Bae, Nack Gyun Chung, Bin Cho, Hack Ki Kim, Chang Ki Min, Chi Wha Han, Ho Shik Kim, Dae Chul Jeong
Departments of Pediatrics, Internal Medicine and Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Korea
Background: The iron chelating agents (ICA) have various biological effects besides iron chelation. We investigated the immunomodulatory effects of Deferasirox (DFS) compared to Deferoxamine (DFO). Methods: Spleen cells (SP) were obtained from 5 week-old C57/BL6 (H-2b). The cytotoxicity of ICAs was examined using the CCK8 method. For the cell proliferation assay, SP were cultured with irradiated in addition to 10, 50, 100ՌM of DFS or DFO and 200ng/mL of cyclosporin A (CSA). Cytokines and nitrite levels were evaluated from supernatants by ELISA. Results: The viability of ICA was reported to be over 100%. Both DFS and DFO inhibited cell proliferation in a manner comparable to CSA. Cell proliferation without iron was reduced at the concentration of 100ՌM of DFO. With iron treatment, the reduction of the stimulation index was dependent on DFO concentrations. DFS decreased the proliferation without reference to the concentrations. After stimulation of phytohemagglutinin, the nitrite concentrations increased with iron. With lipopolysaccharides, the nitrite levels were higher in DFO with iron than control, but similar in DFS regardless of iron treatment. The levels of interleukin-2 were not different. Interleukin-10 was more abundantly produced in 50ՌM of DFO compared to DFS. Transforming growth factor-Ղ was higher in DFS than DFO at the low concentration, but opposite at the high concentration. Conclusion: These data suggested that both iron chelating agents possessed immune suppressive effects comparable to CSA. The immunosuppressive effect of DFS may be distinct from DFO. More experiments are required to determine the exact mechanism of the immunosuppressive effect of DFS. (Korean J Hematol 2008;43:150-158.)
Keywords: Deferoxamine, Deferasirox, Immunosuppressive effect
Jin Hyun Kim, Youn Hee Kim
Korean J Hematol 2008; 43(2): 89-97Sol-Rim Jeon, Jae-Wook Lee, Pil-Sang Jang, Nack-Gyun Chung, Bin Cho, and Dae-Chul Jeong
Blood Res 2015; 50(1): 33-39Jong Gwon Choi, Jung-Lim Kim, Joohee Park, Soonwook Lee, Seh Jong Park, Jun Suk Kim, and Chul Won Choi
Korean J Hematol 2012; 47(3): 194-201