Korean J Hematol 2003; 38(3):
Published online September 30, 2003
© The Korean Society of Hematology
이나리, 송은기, 윤현정, 이호경, 곽재용, 임창열, 정명자, 임현, 최삼임
전북대학교 의과대학 내과학교실,
전북대학교 의과대학 병리과,
전북대학교 의과대학 진단검사의학과
Background: Vascular endothelial growth factor (VEGF) is a potent angiogenic peptide with biologic effects that include regulation of hematopoietic stem cell development, extracellular matrix remodeling, and inflammatory cytokine generation. VEGF plasma levels are elevated in circulation during tumor growth and bone marrow proliferative status. In this study, to investigate the role of VEGF expression in patients with aplastic anemia (AA), VEGF protein expression and microvessel density (MVD) were evaluated.
Methods : Immunohistochemical staining for detecting VEGF protein was performed by the labeled avidin-biotin method on the formalin-fixed and paraffin embedded bone marrow biopsy samples of 25 patients with severe AA and 10 normal controls. Microvessels were scored in at least 3 areas (x200 fields) of the highest MVD in representative sections of each bone marrow biopsy specimen using immunohistochemistry for CD34 antigen.
Results : In AA, megakaryocytes and histocytes expressed less intense cytoplasmic VEGF than in control (P<0.05). However, plasma cells had higher VEGF immunoreactivity in AA than in control. MVD was significantly lower in patients with AA (21.43±7.24), compared to controls (27.65±3.44)(P<0.05). MVD had a strong correlation with bone marrow cellularity. Also, the degree of VEGF immunoreactivity was correlated with bone marrow cellularity and MVD.
Conclusion : Angiogenesis as assessed by MVD and VEGF expression seems to have a role in the pathogenesis of AA.
Keywords Aplastic anemia; VEGF (Vascular endothelial growth factor); MVD (microvessel density)
Korean J Hematol 2003; 38(3): 157-163
Published online September 30, 2003
Copyright © The Korean Society of Hematology.
이나리, 송은기, 윤현정, 이호경, 곽재용, 임창열, 정명자, 임현, 최삼임
전북대학교 의과대학 내과학교실,
전북대학교 의과대학 병리과,
전북대학교 의과대학 진단검사의학과
Na Ri Lee, Eun Kee Song, Hyun Jung Yoon, Ho Kyung Lee, Jae Yong Kwak, Chang Yeol Yim, Myoung Ja Chung, Hyun Lim, Sam Im Choi
Department of Internal Medicine, Pathology, Laboratoty Medicine, Chonbuk National University Medical School and Institute for Medical Science, Chonbuk, Korea
Background: Vascular endothelial growth factor (VEGF) is a potent angiogenic peptide with biologic effects that include regulation of hematopoietic stem cell development, extracellular matrix remodeling, and inflammatory cytokine generation. VEGF plasma levels are elevated in circulation during tumor growth and bone marrow proliferative status. In this study, to investigate the role of VEGF expression in patients with aplastic anemia (AA), VEGF protein expression and microvessel density (MVD) were evaluated.
Methods : Immunohistochemical staining for detecting VEGF protein was performed by the labeled avidin-biotin method on the formalin-fixed and paraffin embedded bone marrow biopsy samples of 25 patients with severe AA and 10 normal controls. Microvessels were scored in at least 3 areas (x200 fields) of the highest MVD in representative sections of each bone marrow biopsy specimen using immunohistochemistry for CD34 antigen.
Results : In AA, megakaryocytes and histocytes expressed less intense cytoplasmic VEGF than in control (P<0.05). However, plasma cells had higher VEGF immunoreactivity in AA than in control. MVD was significantly lower in patients with AA (21.43±7.24), compared to controls (27.65±3.44)(P<0.05). MVD had a strong correlation with bone marrow cellularity. Also, the degree of VEGF immunoreactivity was correlated with bone marrow cellularity and MVD.
Conclusion : Angiogenesis as assessed by MVD and VEGF expression seems to have a role in the pathogenesis of AA.
Keywords: Aplastic anemia, VEGF (Vascular endothelial growth factor), MVD (microvessel density)
Daehun Kwag, Sung‑Soo Park, Sung‑Eun Lee, Hee‑Je Kim and Jong Wook Lee
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