Korean J Hematol 2001; 36(2):
Published online June 30, 2001
© The Korean Society of Hematology
정상 공여자에서 GM-CSF 및 G-CSF 피하주사 후 대용량 백혈구분반술을 이용한 말초혈액 조혈모세포의 채집성적
정진태, 성우진, 채의수, 서광운, 박성원, 곽동석, 김종광, 손상균, 이규보, 권은희, 이난영, 서장수
경북대학교 의과대학 내과학교실,암연구소,
경북대학교 의과대학 임상병리학교실
Peripheral Blood Stem Cell Collection through Large Volume Leukapheresis after Mobilization with GM-CSF and/or G-CSF in Normal Healthy Donors
Background :
The use of colony stimulating factor (CSF) has increased to mobilize hematopoietic progenitor cells for allo-PBSCT. The most effective mobilization regimen has not yet defined. The authors analyzed the results of the mobilized
PBSC collection through large volume leukapheresis from 38 normal healthy donors using three different regimens, namely, a single regimen with GM-CSF (Leucogen®), a concurrent use of GM-CSF and G-CSF (Leucostim®), and a sequential regimen with GM-CSF followed by G-CSF.
Methods : This study was done on 38 healthy donors from Sep. 1998 to Jan. 2001. One donor was mobilized with G-CSF alone, 9 with GM- CSF alone, 20 with concurrent regimen and 8 with sequential regimen. After 5 days of mobilization treatment,
PBSCs were collected by large volume leukapheresis through femoral vein catheter. We compared the results of each collected progenitor cells and observed the side effects.
Results :
The average WBC count before apheresis was 22.6±11.0×10 3 /㎕ and circulating CD34＋ cell percent was 1.31±2.24%. Total 66 times with an average of 1.46±0.61 of large volume leukapheresis were performed for the 37
donors. The mean collected MNC count was 4.61±2.77×10 8 /㎏, CD3＋ cell count was 2.95±1.82×10 8 /㎏ and CD34＋ cell count was 9.76±12.42×10 6 /㎏. A significant side effect observed after large volume leukapheresis was thrombocytopenia showing decrease from 199.1±52.2 to 80.7±25.2×10 3 /㎕ without any bleeding tendency. The mean collected MNC counts provoed to be significantly higher in combination groups with GM-and G-CSF than GM-CSF alone (P<0.05).
The CD34＋ cell counts showed to be statistically higher in a sequential group compared to the concurrent and single regimen groups (P<0.05).
Conclusion :
A mobilization protocol with combination regimens of GM-CSF and G-CSF seemed to be superior to a single regimen with GM-CSF. Large volume leukapheresis through femoral vein catheter after mobilization with combination regimens
of GM-and G-CSF in normal healthy donors was safe and proved to be an excellent. method to harvest stem cells.
Keywords Allogeneic PBSC, Mobilization
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Korean J Hematol 2001; 36(2): 154-161
Published online June 30, 2001
Copyright © The Korean Society of Hematology.
정상 공여자에서 GM-CSF 및 G-CSF 피하주사 후 대용량 백혈구분반술을 이용한 말초혈액 조혈모세포의 채집성적
정진태, 성우진, 채의수, 서광운, 박성원, 곽동석, 김종광, 손상균, 이규보, 권은희, 이난영, 서장수
경북대학교 의과대학 내과학교실,암연구소,
경북대학교 의과대학 임상병리학교실
Peripheral Blood Stem Cell Collection through Large Volume Leukapheresis after Mobilization with GM-CSF and/or G-CSF in Normal Healthy Donors
Jin Tae Jung, Woo Jin Sung, Yee Soo Chae, Kwang Woon Seo, Sung Won Park, Dong Seok Kwak, Jong Gwang Kim, Sang Kyun Sohn, Kyu Bo Lee, Hee Eun Kwon, Nan Young Lee, Jang Soo Suh
Department of Internal Medicine, Clinical Pathology and Cancer Research Center, KyungPook National University, School of Medicine Taegu, Korea
Department of Clinical Medicine, Korea
Abstract
Background :
The use of colony stimulating factor (CSF) has increased to mobilize hematopoietic progenitor cells for allo-PBSCT. The most effective mobilization regimen has not yet defined. The authors analyzed the results of the mobilized
PBSC collection through large volume leukapheresis from 38 normal healthy donors using three different regimens, namely, a single regimen with GM-CSF (Leucogen®), a concurrent use of GM-CSF and G-CSF (Leucostim®), and a sequential regimen with GM-CSF followed by G-CSF.
Methods : This study was done on 38 healthy donors from Sep. 1998 to Jan. 2001. One donor was mobilized with G-CSF alone, 9 with GM- CSF alone, 20 with concurrent regimen and 8 with sequential regimen. After 5 days of mobilization treatment,
PBSCs were collected by large volume leukapheresis through femoral vein catheter. We compared the results of each collected progenitor cells and observed the side effects.
Results :
The average WBC count before apheresis was 22.6±11.0×10 3 /㎕ and circulating CD34＋ cell percent was 1.31±2.24%. Total 66 times with an average of 1.46±0.61 of large volume leukapheresis were performed for the 37
donors. The mean collected MNC count was 4.61±2.77×10 8 /㎏, CD3＋ cell count was 2.95±1.82×10 8 /㎏ and CD34＋ cell count was 9.76±12.42×10 6 /㎏. A significant side effect observed after large volume leukapheresis was thrombocytopenia showing decrease from 199.1±52.2 to 80.7±25.2×10 3 /㎕ without any bleeding tendency. The mean collected MNC counts provoed to be significantly higher in combination groups with GM-and G-CSF than GM-CSF alone (P<0.05).
The CD34＋ cell counts showed to be statistically higher in a sequential group compared to the concurrent and single regimen groups (P<0.05).
Conclusion :
A mobilization protocol with combination regimens of GM-CSF and G-CSF seemed to be superior to a single regimen with GM-CSF. Large volume leukapheresis through femoral vein catheter after mobilization with combination regimens
of GM-and G-CSF in normal healthy donors was safe and proved to be an excellent. method to harvest stem cells.
Keywords: Allogeneic PBSC, Mobilization
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