Blood Res 2021; 56(4):
Published online December 31, 2021
https://doi.org/10.5045/br.2021.2021073
© The Korean Society of Hematology
Correspondence to : Jeong-Ok Lee, M.D., Ph.D.
Division of Hematology and Medical Oncology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam 13620, Korea
E-mail: jeongok77@gmail.com
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background
High-dose chemotherapy followed by autologous stem cell transplantation (HDC-ASCT) as a consolidation treatment is a promising approach for eligible patients with newly diagnosed primary central nervous system lymphoma (PCNSL).
Methods
In this retrospective analysis, 22 patients with newly diagnosed PCNSL received chemotherapy with rituximab, methotrexate, procarbazine, and vincristine. Those who showed complete or partial response subsequently received consolidation HDC-ASCT with a thiotepa- based conditioning regimen but did not undergo radiotherapy.
Results
The PCNSL patients had a median age of 57 years (range, 49‒67 yr); of the total patients, 9.1% had a performance status of 2 or higher, and 72.1% had multiple lesions. Approximately 82% of patients received six cycles of induction chemotherapy, which was well tolerated with excellent disease control. The rate of confirmed or unconfirmed complete response increased from 45.5% at the period of interim analysis to 81.8% prior to the initiation of HDC-ASCT. With a median follow-up of 19.6 months (range, 7.5‒56.5 mo), the 2-year progression-free survival and overall survival estimates were 84% and 88%, respectively. No treatment-related deaths occurred. Grade 3 toxicity was recorded in 90.9% of the patients after undergoing the HDC-ASCT, and the most common grade 3 adverse event was febrile neutropenia without sepsis.
Conclusion
The discussed treatment approach is feasible in patients with newly diagnosed PCNSL, yielding encouraging results.
Keywords Primary CNS lymphoma, Autologous stem-cell transplantation, Consolidation, Thiotepa
Primary central nervous system lymphoma (PCNSL) is an aggressive form of extranodal non-Hodgkin lymphoma that involves the brain, meninges, eyes, and/or spinal cord without systemic involvement [1]. PCNSL accounts for 3% to 4% of all primary brain tumors and 4% to 6% of extranodal lymphomas [2].
First-line induction chemotherapy regimens incorporating high-dose methotrexate (HD-MTX) are considered the standard of care for patients with newly diagnosed PCNSL [3]. HD-MTX is commonly used in combination with other drugs such as vincristine, procarbazine, cytarabine, rituximab, and temozolomide, resulting in high rates of initial response (69–95%) [4-6]. Although PCNSL is sensitive to chemotherapy, more than half of patients who show complete response (CR) will experience relapse within 5 years [7]. Consolidative whole-brain radiotherapy (WBRT) is the most commonly adopted treatment strategy for prolonging remission after induction therapy [8]. However, major concerns regarding neurotoxicity, which can have a tremendous impact on the quality of life of long-term survivors, have prompted the search for alternative consolidative strategies [9].
Previous retrospective and phase II trials have suggested the feasibility and efficacy of high-dose chemotherapy followed by autologous stem-cell transplantation (HDC-ASCT) as a consolidation treatment for patients with PCNSL [10-15]. Most trials addressing HDC-ASCT have included only a small number of patients with a short follow-up period and have demonstrated differences in clinical features and conditioning regimens. In addition, a proportion of patients in these studies also received WBRT as an adjuvant treatment post-ASCT to all patients or as induction therapy to patients who did not achieve a CR. Thus, it is difficult to determine the efficacy and feasibility of HDC-ASCT based on these previous data.
A recent phase II trial of a combination of rituximab, methotrexate, procarbazine, and vincristine (R-MPV) followed by HDC-ASCT with a thiotepa, busulfan, and cyclophosphamide (TBC) conditioning regimen reported excellent disease control, a 2-year progression-free survival (PFS) rate of 79%, and acceptable toxicity profiles with minimal neurotoxicity in patients with newly diagnosed PCNSL [16]. This retrospective study aimed to further investigate the use of R-MPV followed by thiotepa-based HDC-ASCT as consolidation treatment in patients with newly diagnosed PCNSL.
A total of 22 patients with newly diagnosed PCNSL underwent thiotepa-based HDC-ASCT as consolidation therapy at Seoul National University Bundang Hospital between January 2015 and December 2019. All patients had a biopsy-proven diagnosis of PCNSL with diffuse large B-cell lymphoma, except for one patient at risk of postoperative neurologic deficit. The patients’ baseline characteristics obtained for analysis were age, sex, Eastern Cooperative Oncology Group performance status (ECOG PS), lactate dehydrogenase (LDH) levels, cerebrospinal fluid (CSF) protein concentration, and CSF cytological results. The clinical prognostic factors were assessed using the International Extranodal Lymphoma Study Group (IELSG) scoring system [17].
The study was carried out in accordance with the Declaration of Helsinki and was approved by the Institutional Review Board (IRB) of Bundang Hospital. The need for patient consent was waived by the IRB due to the retrospective nature of the study.
All patients received induction chemotherapy with R-MPV, which was administered as follows: day 1, 375 mg/m2 of rituximab; day 2, 3.0 g/m2 of methotrexate (over 3 hr), 1.4 mg/m2 of vincristine; and days 1–7, 100 mg/m2/d of procarbazine (odd cycles only). Hydration therapy and leucovorin rescue were administered according to institutional guidelines. Patients with evidence of malignancy in the CSF received 15 mg of intrathecal methotrexate between cycles. Patients with CR, unconfirmed CR (uCR), or partial response (PR) directly underwent HDC-ASCT.
Meanwhile, patients received busulfan/thiotepa (Bu/TT) or TBC separately as a conditioning chemotherapy regimen. The Bu/TT group received busulfan (3.2 mg/kg intravenously) from day 8 (eight days prior to the initiation of stem cell infusion) to day 5, thiotepa (5 mg/kg intravenously) from day 4 to day 3, and stem cell infusion on day 0. The TBC group received thiotepa (250 mg/m2 intravenously) from day 9 to day 7, busulfan (3.2 mg/kg intravenously) from day 6 to day 4, cyclophosphamide (60 mg/kg intravenously) from day 3 to day 2, and stem cell infusion on day 0. G-CSF was administered daily from day 1 of ASCT until the neutrophil count was <3,000 cells/μL. Neutrophil engraftment and platelet engraftment were defined as an absolute neutrophil count of >0.5×109/L in the first three consecutive days and a platelet count of >20×109/L without transfusion support, respectively. Patients >60 years old or less fit patients based on the clinical judgment of the physician were treated with low-intensity TBC or Bu/TT regimen (Supplementary Table 1).
Response to treatment was assessed using contrast-enhanced brain magnetic resonance imaging (MRI), which was performed after two or three cycles of chemotherapy and before undergoing HDT-ASCT. Treatment response was defined based on the changes in the size of enhanced lesions on T1-weighted MRI and following the National Cancer Institute standardized response criteria [18]. The severity of adverse events was graded based on the National Cancer Institute Common Terminology Criteria for Adverse Events, version 4.0.
The primary outcome measures were response rate, PFS, and overall survival (OS) after diagnosis. OS was defined as the time from initiation of the induction regimen to death from any cause, while PFS was defined as the time from initiation of the induction regimen to treatment failure or death from any cause. The survival curves were estimated using the Kaplan–Meier method and compared using the log-rank test. Univariate analyses were performed to identify the prognostic factors for PFS and OS using a Cox proportional hazards model. A chi-square test was conducted to compare the variables between the two groups as appropriate. All tests were two tailed, and a
The median age of the study group was 57 years (range, 59–67 yr), and 31.8% of the patients were >60 years old. In addition, 59.1% of the patients were men. At baseline, 9.1% of our cohort had an ECOG PS of grade 2 or above, 26.3% had an elevated LDH level, and 95.5% showed an elevated CSF protein concentration. Among the 19 patients in whom complete IELSG PCNSL prognostic parameters were available, 17 (89.5%) presented an intermediate-to-high level of risk. Two patients (9.1%) had a positive CSF cytological result, 13.6% had intraocular lymphoma, and 72.7% had multiple brain lesions. None of the patients had evidence of systemic lymphoma at the time of enrollment (Table 1).
Table 1 Patient’s baseline characteristics (N=22).
Characteristics | N | % |
---|---|---|
Age, median years (range) | 57 (49–67) | |
Age >60 years | 7 | 31.8 |
Male sex | 13 | 59.1 |
ECOG PS ≥2 | 2 | 9.1 |
Elevated LDHa) | 5 | 26.3 |
Elevated CSF proteinb) | 21 | 95.5 |
Deep brain lesions | 16 | 72.7 |
IELSG risk groupa) | ||
Low | 2 | 10.5 |
Intermediate | 14 | 73.7 |
High | 3 | 15.8 |
Positive CSF cytology | 2 | 9.1 |
Ocular involvement | ||
Positive or suspicious | 3 | 13.6 |
Negative | 16 | 72.7 |
Unknown | 3 | 13.6 |
Multiple lesions | 16 | 72.7 |
a)Data regarding serum LDH and IELGS risk groups were available in 19 patients. b)The cutoff values for normal CSF protein con-centration were 45 mg/dL in patients ≤60 years old and 60 mg/dL in patients older than 60 years.
Abbreviations: CSF, cerebrospinal fluid; ECOG PS, Eastern Cooperative Oncology Group performance status; IELSG, international extranodal lymphoma study group; LDH, lactate dehydrogenase.
A majority of patients (18 patients, 81.8%) received six cycles, three patients (13.6%) received five cycles, and one patient (4.5%) received eight cycles of induction chemotherapy. The details of the treatment responses are outlined in Table 2. During interim analysis after induction chemotherapy, 10 patients (45.5%) achieved CR/CRu, while 12 patients (54.5%) achieved PR. At the end of induction chemotherapy, eight of the patients who had previously achieved PR ultimately achieved CR/CRu. Therefore, after completion of induction chemotherapy, 18 patients (81.8%) achieved CR/CRu, while 4 patients (18.2%) achieved PR.
Table 2 Treatment response.
Response | Induction_interim | Pre-HDC-ASCT | Post-HDC-ASCT | Follow-up | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
N | % | N | % | N | % | N | % | ||||
CR | 3 | 13.6 | 8 | 36.4 | 18 | 81.8 | 16 | 72.7 | |||
CRu | 7 | 31.8 | 10 | 45.4 | 4 | 18.2 | 2 | 9.1 | |||
PR | 12 | 54.5 | 4 | 18.2 | 0 | 0 | 0 | 0 | |||
PD | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 18.2 |
Abbreviations: CR, complete response; CRu, CR unconfirmed; HDC-ASCT, high-dose chemotherapy, and autologous stem cell transplant; PD, progressive disease; PR, partial response.
Induction chemotherapy was well tolerated with no reports of treatment-related deaths or treatment discontinuation due to toxicity. Grade 3 and 4 toxicities were noted in 50.5% and 4.5% of patients, respectively. Nine patients (40.9%) developed grade 3 neutropenia, but febrile neutropenia did not occur. Four patients (18.2%) experienced grade 3 hepatic impairment, but their liver enzyme levels returned to baseline values with daily liver function monitoring and receiving hydration therapy. One patient demonstrated grade 4 hepatic impairment after cycle 1; rituximab was omitted during subsequent chemotherapy cycles as it was the only drug that the patient received immediately after experiencing deterioration in liver function.
The characteristics of the HDC-ASCT treatments are summarized in Table 3. All patients underwent therapy with TBC (N=12, 54.5%) or Bu/TT (N=10, 45.5%) as a conditioning regimen followed by ASCT. The median number of infused CD34+ cells was 6.7×106 cells/kg (range, 4.1–21.1×106 cells/kg). The median number of days from treatment to neutrophil engraftment and platelet recovery was 9 days (range, 7–11 days) and 10 days (range, 7–12 days), respectively. The median length of transplantation-related hospitalization was 21 days (range, 18–44 days).
Table 3 HDC-ASCT characteristics.
N | % | |
---|---|---|
Conditioning regimen | ||
TBC | 12 | 54.5 |
Bu/TT | 10 | 45.5 |
Number of infused CD34+ cells (×106 cells/kg), median (range) | 6.7 (4.1–21.1) | |
Neutrophil engraftment, median days (range) | 9 (7–11) | |
Platelet engraftment, median days (range) | 10 (7–12) | |
Transplantation hospitalization, median days (range) | 21 (18–44) |
Abbreviations: Bu/TT, busulfan/thiotepa; TBC, thiotepa, busulfan, and cyclophosphamide.
No treatment-related mortality (TRM) was reported. Grade 3 toxicities were observed in 90.9% of the patients, while none of the patients developed grade 4 toxicities. The most common grade 3 adverse events were febrile neutropenia (86.4%), diarrhea (27.3%), mucositis (22.7%), colitis (18.2%), and nausea (13.6%). Of the 19 patients with febrile neutropenia, 2 had bacteremia but eventually recovered after receiving appropriate antibiotic treatment without sepsis.
The PFS and OS curves are shown in Fig. 1. After a median follow-up period of 19.6 months (range, 7.5–56.5 mo), 19 patients (86.4%) survived and 17 patients (77.3%) had no signs of disease progression. The median PFS and OS were 38.7 months [95% confidence interval (CI), 23.6–49.6] and 43.5 months (95% CI, 31.3–55.7), respectively. The 2-year PFS and OS rates were 84% and 88%, respectively.
Four patients (18.2%) had a tumor relapse in the CNS and received second-line treatment. One patient who experienced a relapse 20 months after transplantation survived after receiving several salvage therapies, while another patient who had a relapse 8 months after transplantation died from progressive lymphoma refractory due to salvage therapy. One patient is currently undergoing WBRT, and another patient died of pneumonia after receiving salvage WBRT. One patient died of aspiration pneumonia, with no evidence of disease recurrence.
In this retrospective study, patients with newly diagnosed PCNSL were treated with R-MPV induction chemotherapy, followed by consolidation HDC-ASCT with a thiotepa-based conditioning regimen in those who showed a response, but did not receive further treatment until disease progression.
With regard to the adapted treatment strategy, there are two major points that should be discussed. The first point is related to the efficacy of R-MPV therapy as induction regimen prior to the initiation of ASCT. Based on the results of retrospective studies comparing historical controls, addition of rituximab to HD-MTX-based chemotherapy improves the chance of CR and OS in patients with newly diagnosed PCNSL [19-21]. Recently, results from the first randomization of the IELSG 32 trial indicated that the addition of rituximab and thiotepa to conventional methotrexate–cytarabine combination therapy (known as the MATRix regimen) was associated with an overall response rate (ORR) of 87% and a 2-year PFS rate of 62% without an increase in the incidence of severe complications [22]. Recent studies have reported that the R-MPV regimen has shown excellent efficacy with CR rates of 69–79% and ORR rates of 95–96% at the end of induction chemotherapy [6, 16]. The present study has limitations inherent to any retrospective analysis and has no data from patients in whom HDC-ASCT was intended but not actually used. Nevertheless, a significant increase in CR/Cru rate was observed during induction chemotherapy, increasing from 45.4% at the time of interim analysis to 81.8% prior to the initiation of HDC-ASCT.
The second point of discussion is the efficacy and tolerability of the conditioning regimen for ASCT. The outcomes of HDC-ASCT as upfront treatment in patients with PCNSL varied in a few single-arm phase 2 trials and retrospective studies (Table 4). Although no formal comparison of conditioning regimens has been conducted to date, historical results gathered after using the BEAM regimen [carmustine (BCNU), etoposide, cytarabine, and melphalan] were disappointing, with a modest treatment response rate and a 2-year OS rate of 60% [10]. According to a previous meta-analysis conducted by Alnahhas
Table 4 HDC-ASCT as upfront treatment in previous studies in PCNSL.
Reference | N | Median age (range) | Induction therapy | CR/ORR to induction | Transplanted patients | Conditioning regimen | WBRT | OS | Median FU (mo) | TRM |
---|---|---|---|---|---|---|---|---|---|---|
[10] | 28 | 53 (25–71) | HD-MTX→ARAC | 29%/50% | 50% | BEAM | No | 2 years: 55% | 28 | 4% |
[15] | 30 | 54 (27–64) | HD-MTX→ ARAC+TT | 20%/70%→38%/92% | 77% | BCNU/TT | Yes | 5 years: 69% | 63 | 3% |
[11] | 25 | 52 (21–60) | MBVP→IFO+ARAC | 44%/84% | 68% | BEAM | Yes | 4 years: 64% | 34 | 4% |
[13] | 23 | 55 (18–69) | HD-MTX | 17%/65% | 70% | Bu/TT | Yes | 2 years: 48% | 15 | 13% |
[12] | 11 | 52 (33–65) | HD-MTX→ARAC | 73%/100% | 100% | BUCYE | Yes | 2 years: 89% | 25 | 0% |
[24] | 21 | 56 (34–69) | MPV→ARAC | 24%/86% | 100% | TBC | No | 5 years: 44% | 60 | 14% |
[14] | 13 | 56 (35–65) | MPV→ARAC | 31%/100% | 46% | LEED | Yes | 3 years: 76% | 44 | 0% |
[16] | 33 | 57 (23–67) | R-MPV | 68%/97% | 81% | TBC | No | 3 years: 81% | 45 | 12% |
Abbreviations: ARAC, cytarabine; BEAM, carmustine, etoposide, cytarabine, and melphalan; BCNU, carmustine; Bu, busulfan; BUCYE, busulfan, cyclophosphamide, and etoposide; CR, complete response; FU, follow-up; HD-MTX, high-dose methotrexate; IFO, ifosfamide; LEED, cyclophosphamide, etoposide, melphalan, and dexamethasone; MBVP, methotrexate, carmustine, etoposide, and methylprednisolone; MPV, methotrexate, vincristine, and procarbazine; ORR, overall response rate; OS, overall survival; R-MP, MPV plus rituximab; TBC, thiotepa, busulfan, and cyclophosphamide; TRM, treatment-related mortality; TT, thiotepa; WBRT, whole-brain radiotherapy.
Despite the significant improvements in the management of PCNSL, nearly half of the responders will relapse, which occurs within 2 years after initial diagnosis [27, 28]. After a median follow-up period of 19.6 months, approximately 18% of patients showed relapse. Although only limited interpretation can be performed due to the small number of patients and short follow-up period, the patients who relapsed within 1 year after ASCT had poorer survival outcomes. Treatment of patients with relapsed and refractory PCNSL remains one of the major unmet clinical needs. The prognosis of R/R PCNSL is extremely poor, with a median OS of 3–5 months [29]. Hence, further clinical trial data are required to guide therapeutic management in this group of patients.
The present study has several limitations. Given the single-center, retrospective nature of this investigation, an undefined bias concerning the clinical outcomes possible exist. Interpretation of the results should be performed with caution due to the small sample size and relatively short follow-up duration; most patients (68%) included in this study were diagnosed from 2018 onward. Our study could not verify the IELSG score for prognostication of survival. Increased CSF protein levels are associated with a poor prognosis [17, 30]. However, adopting CSF parameters as survival predictors remains a problem, given the difficulty of choosing the cutoff value to define unfavorable features. In the IELSG scoring system, the cutoff value for a normal CSF protein concentration was 45 mg/dL in patients aged 60 years or younger but 60 mg/dL in patients aged 60 years or older [17]. When these cutoff values were applied, 95% of patients showed elevated CSF protein concentrations; hence, CSF protein concentration was thought to be unsuitable as a prognostic factor for discrimination in the current study. Different laboratory methodologies account for significant discrepancies across institutions and published reference intervals [31]. In other words, the cutoff value for CSF protein concentration as a predictor of patient’s prognosis cannot be applied uniformly. Hence, further studies using a well-established prognostic scoring system with a better knowledge of PCNSL, especially with the inclusion of histopathologic and molecular variables, are needed. Finally, formal neurocognitive and quality-of-life assessments were not performed.
In conclusion, R-MPV followed by thiotepa-based HDC-ASCT as a consolidation regimen resulted in good response rates and a favorable toxicity profile among patients with newly diagnosed PCNSL.
The authors thank the patients who participated in this trial and their families.
No potential conflicts of interest relevant to this article were reported.
Blood Res 2021; 56(4): 285-292
Published online December 31, 2021 https://doi.org/10.5045/br.2021.2021073
Copyright © The Korean Society of Hematology.
Ji Yun Lee1, Jin Ho Paik2, Koung Jin Suh1, Ji-Won Kim1, Se Hyun Kim1, Jin Won Kim1, Yu Jung Kim1, Keun-Wook Lee1, Jee Hyun Kim1, Soo-Mee Bang1, Jong-Seok Lee1, Jeong-Ok Lee1
1Department of Internal Medicine, 2Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea
Correspondence to:Jeong-Ok Lee, M.D., Ph.D.
Division of Hematology and Medical Oncology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam 13620, Korea
E-mail: jeongok77@gmail.com
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background
High-dose chemotherapy followed by autologous stem cell transplantation (HDC-ASCT) as a consolidation treatment is a promising approach for eligible patients with newly diagnosed primary central nervous system lymphoma (PCNSL).
Methods
In this retrospective analysis, 22 patients with newly diagnosed PCNSL received chemotherapy with rituximab, methotrexate, procarbazine, and vincristine. Those who showed complete or partial response subsequently received consolidation HDC-ASCT with a thiotepa- based conditioning regimen but did not undergo radiotherapy.
Results
The PCNSL patients had a median age of 57 years (range, 49‒67 yr); of the total patients, 9.1% had a performance status of 2 or higher, and 72.1% had multiple lesions. Approximately 82% of patients received six cycles of induction chemotherapy, which was well tolerated with excellent disease control. The rate of confirmed or unconfirmed complete response increased from 45.5% at the period of interim analysis to 81.8% prior to the initiation of HDC-ASCT. With a median follow-up of 19.6 months (range, 7.5‒56.5 mo), the 2-year progression-free survival and overall survival estimates were 84% and 88%, respectively. No treatment-related deaths occurred. Grade 3 toxicity was recorded in 90.9% of the patients after undergoing the HDC-ASCT, and the most common grade 3 adverse event was febrile neutropenia without sepsis.
Conclusion
The discussed treatment approach is feasible in patients with newly diagnosed PCNSL, yielding encouraging results.
Keywords: Primary CNS lymphoma, Autologous stem-cell transplantation, Consolidation, Thiotepa
Primary central nervous system lymphoma (PCNSL) is an aggressive form of extranodal non-Hodgkin lymphoma that involves the brain, meninges, eyes, and/or spinal cord without systemic involvement [1]. PCNSL accounts for 3% to 4% of all primary brain tumors and 4% to 6% of extranodal lymphomas [2].
First-line induction chemotherapy regimens incorporating high-dose methotrexate (HD-MTX) are considered the standard of care for patients with newly diagnosed PCNSL [3]. HD-MTX is commonly used in combination with other drugs such as vincristine, procarbazine, cytarabine, rituximab, and temozolomide, resulting in high rates of initial response (69–95%) [4-6]. Although PCNSL is sensitive to chemotherapy, more than half of patients who show complete response (CR) will experience relapse within 5 years [7]. Consolidative whole-brain radiotherapy (WBRT) is the most commonly adopted treatment strategy for prolonging remission after induction therapy [8]. However, major concerns regarding neurotoxicity, which can have a tremendous impact on the quality of life of long-term survivors, have prompted the search for alternative consolidative strategies [9].
Previous retrospective and phase II trials have suggested the feasibility and efficacy of high-dose chemotherapy followed by autologous stem-cell transplantation (HDC-ASCT) as a consolidation treatment for patients with PCNSL [10-15]. Most trials addressing HDC-ASCT have included only a small number of patients with a short follow-up period and have demonstrated differences in clinical features and conditioning regimens. In addition, a proportion of patients in these studies also received WBRT as an adjuvant treatment post-ASCT to all patients or as induction therapy to patients who did not achieve a CR. Thus, it is difficult to determine the efficacy and feasibility of HDC-ASCT based on these previous data.
A recent phase II trial of a combination of rituximab, methotrexate, procarbazine, and vincristine (R-MPV) followed by HDC-ASCT with a thiotepa, busulfan, and cyclophosphamide (TBC) conditioning regimen reported excellent disease control, a 2-year progression-free survival (PFS) rate of 79%, and acceptable toxicity profiles with minimal neurotoxicity in patients with newly diagnosed PCNSL [16]. This retrospective study aimed to further investigate the use of R-MPV followed by thiotepa-based HDC-ASCT as consolidation treatment in patients with newly diagnosed PCNSL.
A total of 22 patients with newly diagnosed PCNSL underwent thiotepa-based HDC-ASCT as consolidation therapy at Seoul National University Bundang Hospital between January 2015 and December 2019. All patients had a biopsy-proven diagnosis of PCNSL with diffuse large B-cell lymphoma, except for one patient at risk of postoperative neurologic deficit. The patients’ baseline characteristics obtained for analysis were age, sex, Eastern Cooperative Oncology Group performance status (ECOG PS), lactate dehydrogenase (LDH) levels, cerebrospinal fluid (CSF) protein concentration, and CSF cytological results. The clinical prognostic factors were assessed using the International Extranodal Lymphoma Study Group (IELSG) scoring system [17].
The study was carried out in accordance with the Declaration of Helsinki and was approved by the Institutional Review Board (IRB) of Bundang Hospital. The need for patient consent was waived by the IRB due to the retrospective nature of the study.
All patients received induction chemotherapy with R-MPV, which was administered as follows: day 1, 375 mg/m2 of rituximab; day 2, 3.0 g/m2 of methotrexate (over 3 hr), 1.4 mg/m2 of vincristine; and days 1–7, 100 mg/m2/d of procarbazine (odd cycles only). Hydration therapy and leucovorin rescue were administered according to institutional guidelines. Patients with evidence of malignancy in the CSF received 15 mg of intrathecal methotrexate between cycles. Patients with CR, unconfirmed CR (uCR), or partial response (PR) directly underwent HDC-ASCT.
Meanwhile, patients received busulfan/thiotepa (Bu/TT) or TBC separately as a conditioning chemotherapy regimen. The Bu/TT group received busulfan (3.2 mg/kg intravenously) from day 8 (eight days prior to the initiation of stem cell infusion) to day 5, thiotepa (5 mg/kg intravenously) from day 4 to day 3, and stem cell infusion on day 0. The TBC group received thiotepa (250 mg/m2 intravenously) from day 9 to day 7, busulfan (3.2 mg/kg intravenously) from day 6 to day 4, cyclophosphamide (60 mg/kg intravenously) from day 3 to day 2, and stem cell infusion on day 0. G-CSF was administered daily from day 1 of ASCT until the neutrophil count was <3,000 cells/μL. Neutrophil engraftment and platelet engraftment were defined as an absolute neutrophil count of >0.5×109/L in the first three consecutive days and a platelet count of >20×109/L without transfusion support, respectively. Patients >60 years old or less fit patients based on the clinical judgment of the physician were treated with low-intensity TBC or Bu/TT regimen (Supplementary Table 1).
Response to treatment was assessed using contrast-enhanced brain magnetic resonance imaging (MRI), which was performed after two or three cycles of chemotherapy and before undergoing HDT-ASCT. Treatment response was defined based on the changes in the size of enhanced lesions on T1-weighted MRI and following the National Cancer Institute standardized response criteria [18]. The severity of adverse events was graded based on the National Cancer Institute Common Terminology Criteria for Adverse Events, version 4.0.
The primary outcome measures were response rate, PFS, and overall survival (OS) after diagnosis. OS was defined as the time from initiation of the induction regimen to death from any cause, while PFS was defined as the time from initiation of the induction regimen to treatment failure or death from any cause. The survival curves were estimated using the Kaplan–Meier method and compared using the log-rank test. Univariate analyses were performed to identify the prognostic factors for PFS and OS using a Cox proportional hazards model. A chi-square test was conducted to compare the variables between the two groups as appropriate. All tests were two tailed, and a
The median age of the study group was 57 years (range, 59–67 yr), and 31.8% of the patients were >60 years old. In addition, 59.1% of the patients were men. At baseline, 9.1% of our cohort had an ECOG PS of grade 2 or above, 26.3% had an elevated LDH level, and 95.5% showed an elevated CSF protein concentration. Among the 19 patients in whom complete IELSG PCNSL prognostic parameters were available, 17 (89.5%) presented an intermediate-to-high level of risk. Two patients (9.1%) had a positive CSF cytological result, 13.6% had intraocular lymphoma, and 72.7% had multiple brain lesions. None of the patients had evidence of systemic lymphoma at the time of enrollment (Table 1).
Table 1 . Patient’s baseline characteristics (N=22)..
Characteristics | N | % |
---|---|---|
Age, median years (range) | 57 (49–67) | |
Age >60 years | 7 | 31.8 |
Male sex | 13 | 59.1 |
ECOG PS ≥2 | 2 | 9.1 |
Elevated LDHa) | 5 | 26.3 |
Elevated CSF proteinb) | 21 | 95.5 |
Deep brain lesions | 16 | 72.7 |
IELSG risk groupa) | ||
Low | 2 | 10.5 |
Intermediate | 14 | 73.7 |
High | 3 | 15.8 |
Positive CSF cytology | 2 | 9.1 |
Ocular involvement | ||
Positive or suspicious | 3 | 13.6 |
Negative | 16 | 72.7 |
Unknown | 3 | 13.6 |
Multiple lesions | 16 | 72.7 |
a)Data regarding serum LDH and IELGS risk groups were available in 19 patients. b)The cutoff values for normal CSF protein con-centration were 45 mg/dL in patients ≤60 years old and 60 mg/dL in patients older than 60 years..
Abbreviations: CSF, cerebrospinal fluid; ECOG PS, Eastern Cooperative Oncology Group performance status; IELSG, international extranodal lymphoma study group; LDH, lactate dehydrogenase..
A majority of patients (18 patients, 81.8%) received six cycles, three patients (13.6%) received five cycles, and one patient (4.5%) received eight cycles of induction chemotherapy. The details of the treatment responses are outlined in Table 2. During interim analysis after induction chemotherapy, 10 patients (45.5%) achieved CR/CRu, while 12 patients (54.5%) achieved PR. At the end of induction chemotherapy, eight of the patients who had previously achieved PR ultimately achieved CR/CRu. Therefore, after completion of induction chemotherapy, 18 patients (81.8%) achieved CR/CRu, while 4 patients (18.2%) achieved PR.
Table 2 . Treatment response..
Response | Induction_interim | Pre-HDC-ASCT | Post-HDC-ASCT | Follow-up | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
N | % | N | % | N | % | N | % | ||||
CR | 3 | 13.6 | 8 | 36.4 | 18 | 81.8 | 16 | 72.7 | |||
CRu | 7 | 31.8 | 10 | 45.4 | 4 | 18.2 | 2 | 9.1 | |||
PR | 12 | 54.5 | 4 | 18.2 | 0 | 0 | 0 | 0 | |||
PD | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 18.2 |
Abbreviations: CR, complete response; CRu, CR unconfirmed; HDC-ASCT, high-dose chemotherapy, and autologous stem cell transplant; PD, progressive disease; PR, partial response..
Induction chemotherapy was well tolerated with no reports of treatment-related deaths or treatment discontinuation due to toxicity. Grade 3 and 4 toxicities were noted in 50.5% and 4.5% of patients, respectively. Nine patients (40.9%) developed grade 3 neutropenia, but febrile neutropenia did not occur. Four patients (18.2%) experienced grade 3 hepatic impairment, but their liver enzyme levels returned to baseline values with daily liver function monitoring and receiving hydration therapy. One patient demonstrated grade 4 hepatic impairment after cycle 1; rituximab was omitted during subsequent chemotherapy cycles as it was the only drug that the patient received immediately after experiencing deterioration in liver function.
The characteristics of the HDC-ASCT treatments are summarized in Table 3. All patients underwent therapy with TBC (N=12, 54.5%) or Bu/TT (N=10, 45.5%) as a conditioning regimen followed by ASCT. The median number of infused CD34+ cells was 6.7×106 cells/kg (range, 4.1–21.1×106 cells/kg). The median number of days from treatment to neutrophil engraftment and platelet recovery was 9 days (range, 7–11 days) and 10 days (range, 7–12 days), respectively. The median length of transplantation-related hospitalization was 21 days (range, 18–44 days).
Table 3 . HDC-ASCT characteristics..
N | % | |
---|---|---|
Conditioning regimen | ||
TBC | 12 | 54.5 |
Bu/TT | 10 | 45.5 |
Number of infused CD34+ cells (×106 cells/kg), median (range) | 6.7 (4.1–21.1) | |
Neutrophil engraftment, median days (range) | 9 (7–11) | |
Platelet engraftment, median days (range) | 10 (7–12) | |
Transplantation hospitalization, median days (range) | 21 (18–44) |
Abbreviations: Bu/TT, busulfan/thiotepa; TBC, thiotepa, busulfan, and cyclophosphamide..
No treatment-related mortality (TRM) was reported. Grade 3 toxicities were observed in 90.9% of the patients, while none of the patients developed grade 4 toxicities. The most common grade 3 adverse events were febrile neutropenia (86.4%), diarrhea (27.3%), mucositis (22.7%), colitis (18.2%), and nausea (13.6%). Of the 19 patients with febrile neutropenia, 2 had bacteremia but eventually recovered after receiving appropriate antibiotic treatment without sepsis.
The PFS and OS curves are shown in Fig. 1. After a median follow-up period of 19.6 months (range, 7.5–56.5 mo), 19 patients (86.4%) survived and 17 patients (77.3%) had no signs of disease progression. The median PFS and OS were 38.7 months [95% confidence interval (CI), 23.6–49.6] and 43.5 months (95% CI, 31.3–55.7), respectively. The 2-year PFS and OS rates were 84% and 88%, respectively.
Four patients (18.2%) had a tumor relapse in the CNS and received second-line treatment. One patient who experienced a relapse 20 months after transplantation survived after receiving several salvage therapies, while another patient who had a relapse 8 months after transplantation died from progressive lymphoma refractory due to salvage therapy. One patient is currently undergoing WBRT, and another patient died of pneumonia after receiving salvage WBRT. One patient died of aspiration pneumonia, with no evidence of disease recurrence.
In this retrospective study, patients with newly diagnosed PCNSL were treated with R-MPV induction chemotherapy, followed by consolidation HDC-ASCT with a thiotepa-based conditioning regimen in those who showed a response, but did not receive further treatment until disease progression.
With regard to the adapted treatment strategy, there are two major points that should be discussed. The first point is related to the efficacy of R-MPV therapy as induction regimen prior to the initiation of ASCT. Based on the results of retrospective studies comparing historical controls, addition of rituximab to HD-MTX-based chemotherapy improves the chance of CR and OS in patients with newly diagnosed PCNSL [19-21]. Recently, results from the first randomization of the IELSG 32 trial indicated that the addition of rituximab and thiotepa to conventional methotrexate–cytarabine combination therapy (known as the MATRix regimen) was associated with an overall response rate (ORR) of 87% and a 2-year PFS rate of 62% without an increase in the incidence of severe complications [22]. Recent studies have reported that the R-MPV regimen has shown excellent efficacy with CR rates of 69–79% and ORR rates of 95–96% at the end of induction chemotherapy [6, 16]. The present study has limitations inherent to any retrospective analysis and has no data from patients in whom HDC-ASCT was intended but not actually used. Nevertheless, a significant increase in CR/Cru rate was observed during induction chemotherapy, increasing from 45.4% at the time of interim analysis to 81.8% prior to the initiation of HDC-ASCT.
The second point of discussion is the efficacy and tolerability of the conditioning regimen for ASCT. The outcomes of HDC-ASCT as upfront treatment in patients with PCNSL varied in a few single-arm phase 2 trials and retrospective studies (Table 4). Although no formal comparison of conditioning regimens has been conducted to date, historical results gathered after using the BEAM regimen [carmustine (BCNU), etoposide, cytarabine, and melphalan] were disappointing, with a modest treatment response rate and a 2-year OS rate of 60% [10]. According to a previous meta-analysis conducted by Alnahhas
Table 4 . HDC-ASCT as upfront treatment in previous studies in PCNSL..
Reference | N | Median age (range) | Induction therapy | CR/ORR to induction | Transplanted patients | Conditioning regimen | WBRT | OS | Median FU (mo) | TRM |
---|---|---|---|---|---|---|---|---|---|---|
[10] | 28 | 53 (25–71) | HD-MTX→ARAC | 29%/50% | 50% | BEAM | No | 2 years: 55% | 28 | 4% |
[15] | 30 | 54 (27–64) | HD-MTX→ ARAC+TT | 20%/70%→38%/92% | 77% | BCNU/TT | Yes | 5 years: 69% | 63 | 3% |
[11] | 25 | 52 (21–60) | MBVP→IFO+ARAC | 44%/84% | 68% | BEAM | Yes | 4 years: 64% | 34 | 4% |
[13] | 23 | 55 (18–69) | HD-MTX | 17%/65% | 70% | Bu/TT | Yes | 2 years: 48% | 15 | 13% |
[12] | 11 | 52 (33–65) | HD-MTX→ARAC | 73%/100% | 100% | BUCYE | Yes | 2 years: 89% | 25 | 0% |
[24] | 21 | 56 (34–69) | MPV→ARAC | 24%/86% | 100% | TBC | No | 5 years: 44% | 60 | 14% |
[14] | 13 | 56 (35–65) | MPV→ARAC | 31%/100% | 46% | LEED | Yes | 3 years: 76% | 44 | 0% |
[16] | 33 | 57 (23–67) | R-MPV | 68%/97% | 81% | TBC | No | 3 years: 81% | 45 | 12% |
Abbreviations: ARAC, cytarabine; BEAM, carmustine, etoposide, cytarabine, and melphalan; BCNU, carmustine; Bu, busulfan; BUCYE, busulfan, cyclophosphamide, and etoposide; CR, complete response; FU, follow-up; HD-MTX, high-dose methotrexate; IFO, ifosfamide; LEED, cyclophosphamide, etoposide, melphalan, and dexamethasone; MBVP, methotrexate, carmustine, etoposide, and methylprednisolone; MPV, methotrexate, vincristine, and procarbazine; ORR, overall response rate; OS, overall survival; R-MP, MPV plus rituximab; TBC, thiotepa, busulfan, and cyclophosphamide; TRM, treatment-related mortality; TT, thiotepa; WBRT, whole-brain radiotherapy..
Despite the significant improvements in the management of PCNSL, nearly half of the responders will relapse, which occurs within 2 years after initial diagnosis [27, 28]. After a median follow-up period of 19.6 months, approximately 18% of patients showed relapse. Although only limited interpretation can be performed due to the small number of patients and short follow-up period, the patients who relapsed within 1 year after ASCT had poorer survival outcomes. Treatment of patients with relapsed and refractory PCNSL remains one of the major unmet clinical needs. The prognosis of R/R PCNSL is extremely poor, with a median OS of 3–5 months [29]. Hence, further clinical trial data are required to guide therapeutic management in this group of patients.
The present study has several limitations. Given the single-center, retrospective nature of this investigation, an undefined bias concerning the clinical outcomes possible exist. Interpretation of the results should be performed with caution due to the small sample size and relatively short follow-up duration; most patients (68%) included in this study were diagnosed from 2018 onward. Our study could not verify the IELSG score for prognostication of survival. Increased CSF protein levels are associated with a poor prognosis [17, 30]. However, adopting CSF parameters as survival predictors remains a problem, given the difficulty of choosing the cutoff value to define unfavorable features. In the IELSG scoring system, the cutoff value for a normal CSF protein concentration was 45 mg/dL in patients aged 60 years or younger but 60 mg/dL in patients aged 60 years or older [17]. When these cutoff values were applied, 95% of patients showed elevated CSF protein concentrations; hence, CSF protein concentration was thought to be unsuitable as a prognostic factor for discrimination in the current study. Different laboratory methodologies account for significant discrepancies across institutions and published reference intervals [31]. In other words, the cutoff value for CSF protein concentration as a predictor of patient’s prognosis cannot be applied uniformly. Hence, further studies using a well-established prognostic scoring system with a better knowledge of PCNSL, especially with the inclusion of histopathologic and molecular variables, are needed. Finally, formal neurocognitive and quality-of-life assessments were not performed.
In conclusion, R-MPV followed by thiotepa-based HDC-ASCT as a consolidation regimen resulted in good response rates and a favorable toxicity profile among patients with newly diagnosed PCNSL.
The authors thank the patients who participated in this trial and their families.
No potential conflicts of interest relevant to this article were reported.
Table 1 . Patient’s baseline characteristics (N=22)..
Characteristics | N | % |
---|---|---|
Age, median years (range) | 57 (49–67) | |
Age >60 years | 7 | 31.8 |
Male sex | 13 | 59.1 |
ECOG PS ≥2 | 2 | 9.1 |
Elevated LDHa) | 5 | 26.3 |
Elevated CSF proteinb) | 21 | 95.5 |
Deep brain lesions | 16 | 72.7 |
IELSG risk groupa) | ||
Low | 2 | 10.5 |
Intermediate | 14 | 73.7 |
High | 3 | 15.8 |
Positive CSF cytology | 2 | 9.1 |
Ocular involvement | ||
Positive or suspicious | 3 | 13.6 |
Negative | 16 | 72.7 |
Unknown | 3 | 13.6 |
Multiple lesions | 16 | 72.7 |
a)Data regarding serum LDH and IELGS risk groups were available in 19 patients. b)The cutoff values for normal CSF protein con-centration were 45 mg/dL in patients ≤60 years old and 60 mg/dL in patients older than 60 years..
Abbreviations: CSF, cerebrospinal fluid; ECOG PS, Eastern Cooperative Oncology Group performance status; IELSG, international extranodal lymphoma study group; LDH, lactate dehydrogenase..
Table 2 . Treatment response..
Response | Induction_interim | Pre-HDC-ASCT | Post-HDC-ASCT | Follow-up | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
N | % | N | % | N | % | N | % | ||||
CR | 3 | 13.6 | 8 | 36.4 | 18 | 81.8 | 16 | 72.7 | |||
CRu | 7 | 31.8 | 10 | 45.4 | 4 | 18.2 | 2 | 9.1 | |||
PR | 12 | 54.5 | 4 | 18.2 | 0 | 0 | 0 | 0 | |||
PD | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 18.2 |
Abbreviations: CR, complete response; CRu, CR unconfirmed; HDC-ASCT, high-dose chemotherapy, and autologous stem cell transplant; PD, progressive disease; PR, partial response..
Table 3 . HDC-ASCT characteristics..
N | % | |
---|---|---|
Conditioning regimen | ||
TBC | 12 | 54.5 |
Bu/TT | 10 | 45.5 |
Number of infused CD34+ cells (×106 cells/kg), median (range) | 6.7 (4.1–21.1) | |
Neutrophil engraftment, median days (range) | 9 (7–11) | |
Platelet engraftment, median days (range) | 10 (7–12) | |
Transplantation hospitalization, median days (range) | 21 (18–44) |
Abbreviations: Bu/TT, busulfan/thiotepa; TBC, thiotepa, busulfan, and cyclophosphamide..
Table 4 . HDC-ASCT as upfront treatment in previous studies in PCNSL..
Reference | N | Median age (range) | Induction therapy | CR/ORR to induction | Transplanted patients | Conditioning regimen | WBRT | OS | Median FU (mo) | TRM |
---|---|---|---|---|---|---|---|---|---|---|
[10] | 28 | 53 (25–71) | HD-MTX→ARAC | 29%/50% | 50% | BEAM | No | 2 years: 55% | 28 | 4% |
[15] | 30 | 54 (27–64) | HD-MTX→ ARAC+TT | 20%/70%→38%/92% | 77% | BCNU/TT | Yes | 5 years: 69% | 63 | 3% |
[11] | 25 | 52 (21–60) | MBVP→IFO+ARAC | 44%/84% | 68% | BEAM | Yes | 4 years: 64% | 34 | 4% |
[13] | 23 | 55 (18–69) | HD-MTX | 17%/65% | 70% | Bu/TT | Yes | 2 years: 48% | 15 | 13% |
[12] | 11 | 52 (33–65) | HD-MTX→ARAC | 73%/100% | 100% | BUCYE | Yes | 2 years: 89% | 25 | 0% |
[24] | 21 | 56 (34–69) | MPV→ARAC | 24%/86% | 100% | TBC | No | 5 years: 44% | 60 | 14% |
[14] | 13 | 56 (35–65) | MPV→ARAC | 31%/100% | 46% | LEED | Yes | 3 years: 76% | 44 | 0% |
[16] | 33 | 57 (23–67) | R-MPV | 68%/97% | 81% | TBC | No | 3 years: 81% | 45 | 12% |
Abbreviations: ARAC, cytarabine; BEAM, carmustine, etoposide, cytarabine, and melphalan; BCNU, carmustine; Bu, busulfan; BUCYE, busulfan, cyclophosphamide, and etoposide; CR, complete response; FU, follow-up; HD-MTX, high-dose methotrexate; IFO, ifosfamide; LEED, cyclophosphamide, etoposide, melphalan, and dexamethasone; MBVP, methotrexate, carmustine, etoposide, and methylprednisolone; MPV, methotrexate, vincristine, and procarbazine; ORR, overall response rate; OS, overall survival; R-MP, MPV plus rituximab; TBC, thiotepa, busulfan, and cyclophosphamide; TRM, treatment-related mortality; TT, thiotepa; WBRT, whole-brain radiotherapy..
Jeong Eun Kim, Dok Hyun Yoon, Shin Kim, Dae Ho Lee, Jeong Hoon Kim, Young Hee Yoon, Hyun Sook Chi, Sang Wook Lee, Chan-Sik Park, Jooryung Huh, and Cheolwon Suh
Korean J Hematol 2012; 47(1): 60-66Hee Je Kim, Jong Wook Lee, Soo JeongPark, Jung Gon Suh, ChangKi Min, Hyeon Seok Eom, Dong Wook Kim, Woo Sung Min, Chun Choo Kim, Dong Jip Kim
Korean J Hematol 1999; 34(2): 288-296