Blood Res 2021; 56(4):
Published online December 31, 2021
https://doi.org/10.5045/br.2021.2020331
© The Korean Society of Hematology
Correspondence to : Jongha Yoo
Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang 10444, Korea
E-mail: jhyoo92@nhimc.or.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
TO THE EDITOR: Minor
An 81-year-old male with a history of hypertension and dyslipidemia was referred to our hospital because of marked leukocytosis. The complete blood count (CBC) at referral indicated anemia, leukocytosis, and thrombocytopenia, with a hemoglobin (Hb) level of 7.5 g/dL, white blood cell (WBC) count of 37.59×109/L, and a platelet count of 89×109/L. The differential counts of WBC were myelocytes 1%, metamyelocytes 2%, band neutrophils 13%, neutrophils 45%, eosinophils 2%, basophils 6%, lymphocytes 12%, and monocytes 19% (Fig. 1). Bone marrow (BM) biopsy and related cytogenetic studies were performed; bone marrow biopsy showed hypercellularity, with an estimated cellularity of 70–90% (Fig. 2) with granulocytic proliferation. Additionally, the number of megakaryocytes and dwarf megakaryocytes had increased (Fig. 3). Approximately 5.3% of all nucleated cells (ANCs) were counted as blasts, whereas the population co-expressing CD34(+), CD117(+), and myeloperoxidase corresponding to myeloblasts, was 2.28% of the total cells observed using flow cytometry (FCM). Pseudo-Gaucher cells were not observed. Monocytes accounted for 17.1% of the ANCs, consistent with the FCM results of 17.4%. Conventional chromosome analysis using BM cells exhibited 46,XY,t(9;22)(q34;q11.2)[20] (Fig. 4). Reverse transcriptase-polymerase chain reaction (RT-PCR) showed a minor (P190)
The Philadelphia chromosome, resulting from the chromosomal translocation t(9;22), contains the
The case we have described here constitutes the second case of minor
No potential conflicts of interest relevant to this article were reported.
Blood Res 2021; 56(4): 345-347
Published online December 31, 2021 https://doi.org/10.5045/br.2021.2020331
Copyright © The Korean Society of Hematology.
Yu Jeong Choi1, Ja Yoon Heo2, Jongha Yoo3
1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, 2Division of Hematology-Oncology, Department of Internal Medicine, 3Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea
Correspondence to:Jongha Yoo
Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang 10444, Korea
E-mail: jhyoo92@nhimc.or.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
TO THE EDITOR: Minor
An 81-year-old male with a history of hypertension and dyslipidemia was referred to our hospital because of marked leukocytosis. The complete blood count (CBC) at referral indicated anemia, leukocytosis, and thrombocytopenia, with a hemoglobin (Hb) level of 7.5 g/dL, white blood cell (WBC) count of 37.59×109/L, and a platelet count of 89×109/L. The differential counts of WBC were myelocytes 1%, metamyelocytes 2%, band neutrophils 13%, neutrophils 45%, eosinophils 2%, basophils 6%, lymphocytes 12%, and monocytes 19% (Fig. 1). Bone marrow (BM) biopsy and related cytogenetic studies were performed; bone marrow biopsy showed hypercellularity, with an estimated cellularity of 70–90% (Fig. 2) with granulocytic proliferation. Additionally, the number of megakaryocytes and dwarf megakaryocytes had increased (Fig. 3). Approximately 5.3% of all nucleated cells (ANCs) were counted as blasts, whereas the population co-expressing CD34(+), CD117(+), and myeloperoxidase corresponding to myeloblasts, was 2.28% of the total cells observed using flow cytometry (FCM). Pseudo-Gaucher cells were not observed. Monocytes accounted for 17.1% of the ANCs, consistent with the FCM results of 17.4%. Conventional chromosome analysis using BM cells exhibited 46,XY,t(9;22)(q34;q11.2)[20] (Fig. 4). Reverse transcriptase-polymerase chain reaction (RT-PCR) showed a minor (P190)
The Philadelphia chromosome, resulting from the chromosomal translocation t(9;22), contains the
The case we have described here constitutes the second case of minor
No potential conflicts of interest relevant to this article were reported.