Investigating the expression pattern of the angiopoietin-Tie system in ALL and its correlation with baseline characteristics
Saeed Zaka Khosravi1,2, Samira Molaei Ramshe3, Mehdi Allahbakhshian Farsani4, Saeed Solali2, Mohammadreza Moonesi1,2, Majid Farshdousti Hagh1,5
1Immunology Research Center, 2Division of Hematology and Transfusion Medicine, Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, 3Student Research Committee, Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, 4HSCT Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Correspondence to: Majid Farshdousti Hagh, Ph.D.
Immunology Research Center, Tabriz University of Medical Sciences, Golgasht Street, Tabriz 5166/15731, Iran
Email: m.farshdousti@gmail.com
Published online: May 25, 2021.
© The Korean Journal of Hematology. All rights reserved.

Abstract
Background: Acute lymphoblastic leukemia (ALL) is the most common type of leukemia in children. Several environmental and genetic factors are known to be involved in its development and progression. The angiopoietin-Tie system is one of the most critical factors in angiogenesis, and its possible role in solid tumors and leukemia has been previously investigated. In this study, we examined the expression of these genes in ALL patients (early pre-B-ALL and pre-B-ALL) and compared them with normal samples.
Methods: Bone marrow samples were collected from 40 patients (aged 0–19 yr) newly diagnosed with early pre-B-ALL or pre-B-ALL using molecular and flow cytometric tests and from 15 control individuals. For molecular tests, RNA extraction and cDNA synthesis were performed, and Ang1, Ang2, Ang4, Tie1, and Tie2 gene expression was examined by real-time polymerase chain reaction.
Results: Ang2, Tie1, and Tie2 gene expression were significantly increased in patients with ALL, whereas Ang1 gene expression was decreased. The Ang4 gene did not show significant expression changes between the two groups.
Conclusion: Changes in the expression of the Ang-Tie system indicate a possible role of angiogenesis in ALL prognosis. Moreover, such changes can be considered as potential diagnostic biomarkers or therapeutic targets.
Keywords: Angiopoietin, Leukemia, Tie receptor, Acute lymphoblastic leukemia


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