Blood Res 2020; 55(2):
Published online June 30, 2020
https://doi.org/10.5045/br.2020.2020047
© The Korean Society of Hematology
Correspondence to : Ankur Jain
Department of Haematology, Vardhman Mahavir Medical College and Safdarjung Hospital, Mahatma Gandhi Marg, New Delhi 110029, India
E-mail: drankur589@yahoo.in
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
A 76-year-old man presented to our hospital with a 3-month history of insidious onset of weakness involving both the upper limbs, with associated numbness up to the elbows. Weakness progressed gradually to involve both the lower limbs. Patient could not stand unsupported after 1 month. The patient’s clinical history was negative for diabetes, hypertension, alcohol intake, and autoimmune disorder. General examination revealed bilateral wrist drop and wasting of bilateral thenar and hypothenar eminences. Neurological exam was remarkable for bilateral distal upper limb hypotonia, areflexia, impaired vibration, and proprioception. The examination of motor system of the lower limbs was essentially normal; however, distal vibration and proprioception were impaired up to the knees. The cranial nerve exam was normal. The liver (4 cm) and spleen (2 cm) were palpable per abdomen. Blood tests showed the following: hemoglobin, 124 g/L; white cell counts, 16.30×109/L (lymphocytes-80%); and platelet counts, 89×109/L. Peripheral blood smear showed presence of small-medium sized lymphocytes and smudge cells. Prolymphocytes were not seen. Organ chemistries, including serum vitamin B12, red cell folate, and C-reactive protein levels were normal. Serum lactate dehydrogenase was slightly elevated (283 U/L; normal, <250 U/L). Viral markers [hepatitis B surface antigen, anti-hepatitis C virus (HCV) and anti-human immunodeficiency virus (HIV) serology, Epstein-Barr virus, and Cytomegalovirus DNA] were negative. BM biopsy showed interstitial infiltration by small-medium sized mature lymphocytes with scant cytoplasm and clumped chromatin. Flow cytometry (FCM) of BM aspirate showed increased clonal B-lymphocyte phenotype: CD45+, CD19+, CD20+, FMC-7+, CD200+, CD38+, CD5-, CD23-, CD10-, CD11c-, CD25-, CD103-, and CD123-. Morphology and immunophenotype were consistent with a diagnosis of atypical chronic lymphocytic leukemia (aCLL) (Fig. 1). BM cytogenetics revealed normal karyotype. Serum and urine protein electrophoresis and immunofixation were negative. Serum free light chain (sFLC) levels were as follows: kappa, 90.0 mg/L (3.30–19.40 mg/L); lambda, 25.90 mg/L (5.71–26.30 mg/L); and ratio, 3.47 (0.26–1.65). Immunoglobulin profile was normal [IgG, 8.9 g/L (6.6–16.9 g/L); IgM, 0.32 g/L (0.37–2.58 g/L); and IgA, 1.2 g/L (1.03–5.91 g/L)]. 18Fluoro-2-deoxy-d-glucose positron emission tomography–computed tomography (18FDG PET-CT) scan failed to show any abnormality. Nerve conduction study (NCS) was consistent with the diagnosis of multifocal acquired demyelinating sensory and motor (MADSAM) neuropathy. Cerebrospinal fluid (CSF) examination was normal. Autoimmune panel, including antinuclear antibody, anti-neutrophil cytoplasmic antibodies, and PN antibody panel (anti-GM1, GM2, GM3, GD1a, GD1b, GT1b, GQ1b, MAG) were negative. Abdominal fat pad biopsy did not reveal any amyloid deposits. Serum cryoglobulins were not detected. FCM of peripheral blood identified a normal subset of regulatory T-cells (Tregs). Patient was treated with rituximab monotherapy (375 mg/m2/wk ×4 doses). He experienced marked improvement in his neurological function, beginning after the first rituximab dose. He could stand and walk unsupported and had about 80% improvement in hand grip at the end of fourth rituximab dose. However, neuropathy progressed 2 weeks later, and he failed to respond to intravenous immunoglobulin treatment. Treatment with high-dose methylprednisolone (MPS, 1 gm/day ×3 days) halted the neurological worsening. Reassessment after MPS therapy suggested regression of organomegaly, absence of clonal B-cells in the peripheral blood and BM aspirate by FCM, and normalization of sFLC. Maintenance rituximab (every 2 months) resulted in an ongoing neurological improvement.
Mechanistically, M-protein can inflict PN either 1) directly, by means of tissue deposition and consequent neurological impairment, or 2) indirectly, by acting as an autoantibody directed against peripheral nerve antigens [1]. Whilst the former category includes amyloidosis-associated PN and cryoglobulinemic neuropathy (CN), the latter is exemplified by (a) distal acquired demyelinating symmetric neuropathy (DADS, IgM-MG, anti-MAG antibody), (b) chronic inflammatory demyelinating polyneuropathy (CIDP, IgG/IgA-MG), (c) chronic ataxic neuropathy with ophthalmoparesis, M-protein, cold agglutinins, and disialosyl ganglioside antibodies syndrome (CANOMAD syndrome, usually IgM-MG), and (d) polyneuropathy, organomegaly, endocrinopathy, monoclonal disorder, and skin changes (POEMS syndrome, vascular endothelial growth factor-mediated) [1, 5]. Association of MADSAM with MG has not been described [6]. CLL-like morphology, a low Matutes score, and CD200-positivity supported the diagnosis of aCLL in this case [7, 8]. Follicular lymphoma was unlikely because of CD10-negativity, and CD200-positivity excluded mantle cell and marginal zone lymphoma. Absence of plasmacytic differentiation and CD200-positivity argued against lymphoplasmacytic lymphoma (LPL). Absence of hairy cell markers excluded hairy cell leukemia [8, 9]. An abnormal sFLC ratio might not necessarily imply monoclonality and could happen in the setting of renal impairment or polyclonal FLC increase secondary to infection and inflammation [10]. Normal renal function test and immunoglobulin levels made these possibilities unlikely in our case. After exclusion of the polyclonal causes, abnormal sFLC ratio could suggest monoclonality, even if results of serum/urine electrophoresis and immunofixation are negative [11]. Our patient had B-CLPD (aCLL), evidence of monoclonality (abnormal sFLC ratio), and clinical and electrophysiological findings, suggesting MADSAM. Workup for amyloidosis and cryoglobulinemia was negative. Negative PN antibody panel was consistent with MADSAM [6]. Autoimmune manifestations including PN are well known in CLPD [12]. Normal Tregs and a negative autoimmune panel argued against this possibility. Absence of neural thickening or enhancement on 18FDG PET-CT scan and normal CSF analysis make consideration of neurolymphomatosis unlikely. Either direct toxicity or autoantibody property of kappa light chains could have been responsible for PN in our case. Rapid clinical response seen with single agent rituximab, paralleled by decline in clonal B-cells and normalization of sFLC indicates MG-driven neural damage. Clinical response deepened with maintenance rituximab, possibly suggesting ongoing clonal eradication with possible neural regeneration. Recently, Chen
No potential conflicts of interest relevant to this article were reported.
Blood Res 2020; 55(2): 117-120
Published online June 30, 2020 https://doi.org/10.5045/br.2020.2020047
Copyright © The Korean Society of Hematology.
Ankur Jain, Pooja Prasad, Sumita Chaudhry, Dipendra Kumar Gupta, Sumita Saluja
Department of Haematology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India
Correspondence to:Ankur Jain
Department of Haematology, Vardhman Mahavir Medical College and Safdarjung Hospital, Mahatma Gandhi Marg, New Delhi 110029, India
E-mail: drankur589@yahoo.in
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
A 76-year-old man presented to our hospital with a 3-month history of insidious onset of weakness involving both the upper limbs, with associated numbness up to the elbows. Weakness progressed gradually to involve both the lower limbs. Patient could not stand unsupported after 1 month. The patient’s clinical history was negative for diabetes, hypertension, alcohol intake, and autoimmune disorder. General examination revealed bilateral wrist drop and wasting of bilateral thenar and hypothenar eminences. Neurological exam was remarkable for bilateral distal upper limb hypotonia, areflexia, impaired vibration, and proprioception. The examination of motor system of the lower limbs was essentially normal; however, distal vibration and proprioception were impaired up to the knees. The cranial nerve exam was normal. The liver (4 cm) and spleen (2 cm) were palpable per abdomen. Blood tests showed the following: hemoglobin, 124 g/L; white cell counts, 16.30×109/L (lymphocytes-80%); and platelet counts, 89×109/L. Peripheral blood smear showed presence of small-medium sized lymphocytes and smudge cells. Prolymphocytes were not seen. Organ chemistries, including serum vitamin B12, red cell folate, and C-reactive protein levels were normal. Serum lactate dehydrogenase was slightly elevated (283 U/L; normal, <250 U/L). Viral markers [hepatitis B surface antigen, anti-hepatitis C virus (HCV) and anti-human immunodeficiency virus (HIV) serology, Epstein-Barr virus, and Cytomegalovirus DNA] were negative. BM biopsy showed interstitial infiltration by small-medium sized mature lymphocytes with scant cytoplasm and clumped chromatin. Flow cytometry (FCM) of BM aspirate showed increased clonal B-lymphocyte phenotype: CD45+, CD19+, CD20+, FMC-7+, CD200+, CD38+, CD5-, CD23-, CD10-, CD11c-, CD25-, CD103-, and CD123-. Morphology and immunophenotype were consistent with a diagnosis of atypical chronic lymphocytic leukemia (aCLL) (Fig. 1). BM cytogenetics revealed normal karyotype. Serum and urine protein electrophoresis and immunofixation were negative. Serum free light chain (sFLC) levels were as follows: kappa, 90.0 mg/L (3.30–19.40 mg/L); lambda, 25.90 mg/L (5.71–26.30 mg/L); and ratio, 3.47 (0.26–1.65). Immunoglobulin profile was normal [IgG, 8.9 g/L (6.6–16.9 g/L); IgM, 0.32 g/L (0.37–2.58 g/L); and IgA, 1.2 g/L (1.03–5.91 g/L)]. 18Fluoro-2-deoxy-d-glucose positron emission tomography–computed tomography (18FDG PET-CT) scan failed to show any abnormality. Nerve conduction study (NCS) was consistent with the diagnosis of multifocal acquired demyelinating sensory and motor (MADSAM) neuropathy. Cerebrospinal fluid (CSF) examination was normal. Autoimmune panel, including antinuclear antibody, anti-neutrophil cytoplasmic antibodies, and PN antibody panel (anti-GM1, GM2, GM3, GD1a, GD1b, GT1b, GQ1b, MAG) were negative. Abdominal fat pad biopsy did not reveal any amyloid deposits. Serum cryoglobulins were not detected. FCM of peripheral blood identified a normal subset of regulatory T-cells (Tregs). Patient was treated with rituximab monotherapy (375 mg/m2/wk ×4 doses). He experienced marked improvement in his neurological function, beginning after the first rituximab dose. He could stand and walk unsupported and had about 80% improvement in hand grip at the end of fourth rituximab dose. However, neuropathy progressed 2 weeks later, and he failed to respond to intravenous immunoglobulin treatment. Treatment with high-dose methylprednisolone (MPS, 1 gm/day ×3 days) halted the neurological worsening. Reassessment after MPS therapy suggested regression of organomegaly, absence of clonal B-cells in the peripheral blood and BM aspirate by FCM, and normalization of sFLC. Maintenance rituximab (every 2 months) resulted in an ongoing neurological improvement.
Mechanistically, M-protein can inflict PN either 1) directly, by means of tissue deposition and consequent neurological impairment, or 2) indirectly, by acting as an autoantibody directed against peripheral nerve antigens [1]. Whilst the former category includes amyloidosis-associated PN and cryoglobulinemic neuropathy (CN), the latter is exemplified by (a) distal acquired demyelinating symmetric neuropathy (DADS, IgM-MG, anti-MAG antibody), (b) chronic inflammatory demyelinating polyneuropathy (CIDP, IgG/IgA-MG), (c) chronic ataxic neuropathy with ophthalmoparesis, M-protein, cold agglutinins, and disialosyl ganglioside antibodies syndrome (CANOMAD syndrome, usually IgM-MG), and (d) polyneuropathy, organomegaly, endocrinopathy, monoclonal disorder, and skin changes (POEMS syndrome, vascular endothelial growth factor-mediated) [1, 5]. Association of MADSAM with MG has not been described [6]. CLL-like morphology, a low Matutes score, and CD200-positivity supported the diagnosis of aCLL in this case [7, 8]. Follicular lymphoma was unlikely because of CD10-negativity, and CD200-positivity excluded mantle cell and marginal zone lymphoma. Absence of plasmacytic differentiation and CD200-positivity argued against lymphoplasmacytic lymphoma (LPL). Absence of hairy cell markers excluded hairy cell leukemia [8, 9]. An abnormal sFLC ratio might not necessarily imply monoclonality and could happen in the setting of renal impairment or polyclonal FLC increase secondary to infection and inflammation [10]. Normal renal function test and immunoglobulin levels made these possibilities unlikely in our case. After exclusion of the polyclonal causes, abnormal sFLC ratio could suggest monoclonality, even if results of serum/urine electrophoresis and immunofixation are negative [11]. Our patient had B-CLPD (aCLL), evidence of monoclonality (abnormal sFLC ratio), and clinical and electrophysiological findings, suggesting MADSAM. Workup for amyloidosis and cryoglobulinemia was negative. Negative PN antibody panel was consistent with MADSAM [6]. Autoimmune manifestations including PN are well known in CLPD [12]. Normal Tregs and a negative autoimmune panel argued against this possibility. Absence of neural thickening or enhancement on 18FDG PET-CT scan and normal CSF analysis make consideration of neurolymphomatosis unlikely. Either direct toxicity or autoantibody property of kappa light chains could have been responsible for PN in our case. Rapid clinical response seen with single agent rituximab, paralleled by decline in clonal B-cells and normalization of sFLC indicates MG-driven neural damage. Clinical response deepened with maintenance rituximab, possibly suggesting ongoing clonal eradication with possible neural regeneration. Recently, Chen
No potential conflicts of interest relevant to this article were reported.