The Jak-2 inhibitor ruxolitinib reduces constitutional symptoms and splenomegaly in patients with myelofibrosis and has been shown to increase overall survival [1 2]. The JAK-STAT pathway has an important role in host defense and autoimmunity. There are ongoing trials using ruxolitinib as an immune modulator for ameliorating graft versus host disease in allogeneic hematopoietic stem cell transplant (allo-HCT). Recently, attention has been drawn to the increase in opportunistic infections and viral reactivations in patients receiving ruxolitinib . Here, we describe a patient receiving ruxolitinib for myelofibrosis who was diagnosed with symptomatic Epstein-Barr virus (EBV) reactivation.
Our patient had a history of essential thrombocythemia, which was treated with anagrelide and hydroxyurea, and it progressed to Jak-2 positive myelofibrosis over a 20-year period. He had high-risk myelofibrosis according to the MIPSS 70 scoring system due to high white blood cell (WBC) count (around 25−30×109/µL), red cell transfusion dependence, circulating peripheral blood blasts, and presence of
There was no objective evidence of disease as response to ruxolitinib, and he continued to require transfusions; however, the transfusion frequency had decreased after splenectomy. Due to the high-risk features of his disease, he was scheduled for allo-HCT. On admission for the same treatment (around 14 months after treatment with ruxolitinib was first started), he was found to have altered liver function test (LFT) and palpable cervical lymphadenopathy (ALP 644 IU/L, total bilirubin 4 mg/dL, SGOT 57 IU/L, and SGPT 89 IU/L). The blood counts at the time revealed a hemoglobin of 8 g/dL, platelet count 136×109/µL, total WBC count 75.7×109/µL, neutrophils 4.8×109/µL, lymphocytes/monocytes 65×109/µL, blast count 2×109/µL, basophils 1.4×109/µL, meta-myelocytes 0.7×109/µL, myelocytes 0.7×109/µL, and pro-myelocytes 0.7×109/µL. Peripheral blood lymphocyte count had increased rapidly to 65×109/µL (previous lymphocyte count ranged from 1.5 to 15×109/µL), and he was found to have EBV reactivation with 824,000 copies/mL which was confirmed on repeat EBV assay (he had a documented negative EBV PCR 3 months prior to this event). He did not have any concomitant viral infections at that time. Cervical lymph node biopsy showed extramedullary hematopoiesis and was negative for EBV antigens on immunohistochemistry. Computed tomography of the chest and abdomen showed an increase in the size of pre-existing para-aortic and mesenteric lymphadenopathy and ill-defined hepatic lesions. Flow cytometry to further delineate the etiology of peripheral blood lymphocytosis revealed the following: T lymphocytes, 41%; B lymphocytes, <1%; monocytes, 35%; natural killer (NK) cells, <1%; and a low CD4/CD8 ratio of 1.26; this was suggestive of a reactive lymphocyte population. Considering the elevated EBV viral load, altered LFT, and nodal and hepatic lesions, the patient was diagnosed with reactive lymphocytosis secondary to EBV reactivation. Ruxolitinib had already been tapered and discontinued 1 day prior to his admission for allo-SCT, and he was treated with rituximab 375 mg/m2. The trends in total white blood cell count and lymphocyte count prior to, during, and in the first 7 days after detection of EBV reactivation and receiving rituximab are shown in Fig. 1. His lymphocyte count and liver enzymes returned to baseline levels over the next 2 weeks. The repeat PCR was negative for EBV 1 week later, and the patient was re-admitted for an allo-HCT 2 weeks after his liver enzyme levels had returned to baseline.
There is no mention of EBV in the JAKAVI (ruxolitinib) product monograph , though the “serious warnings and precautions” section in the monograph states that serious bacterial, mycobacterial, fungal, and viral infections (in some cases fatal or rare such as progressive multifocal leukoencephalopathy) have been reported in patients receiving ruxolitinib.
In the pivotal COMFORT-I and COMFORT-II trials on ruxolitinib in myelofibrosis [1 2], herpes zoster infections were reported at higher rates in patients treated with ruxolitinib than in those treated with the placebo, and the incidence increased with increase in the duration of exposure (0−12 mo exposure −2.1%; ≥48 mo exposure −10.3%). Other infections, including pneumonia, sepsis, upper respiratory tract infection, and urinary tract infection occurred at similar or lower rates in ruxolitinib-treated patients than in those treated with the placebo. Pneumonia was the most common new-onset grade 3 or 4 adverse event observed after 48 months of treatment. Leukocyte subpopulations, lymphocyte functions, or antibody deficiency were not documented in these studies.
There are two cases of EBV infection associated with ruxolitinib reported in the literature [5 6]. The first was a rapidly fatal, EBV-driven lymphoproliferative disorder of the central nervous system in a patient with post-polycythemia myelofibrosis 9 weeks after starting ruxolitinib. The patient had a documented normal brain magnetic resonance imaging 3 weeks prior to initiation of ruxolitinib suggesting that the disorder developed after starting ruxolitinib. The second report was that of a patient with myelofibrosis receiving ruxolitinib, who presented with severe diarrhea related to a gastric ulcer, which was positive for EBV by PCR in biopsy but negative in the blood. In this patient, the authors were able to document a serial decrease in the number of NK cells and CD4+ T cells in the patient's blood while receiving ruxolitinib. The EBV infection responded to ganciclovir, and ruxolitinib was discontinued, but the level of T and NK cells continued to decline for the next 3 months after stopping ruxolitinib.
Even though we did not have pathological evidence of EBV on lymph node biopsy in our patient, the acute lymphoproliferative response was seen in association with the rise in serum bilirubin and alkaline phosphatase, which is similar to a post-transplant lymphoproliferative disorder seen in association with EBV in immunocompromised patients.
JAK mutations are associated with primary immunodeficiency, and complete STAT-1 deficiency can lead to lethal viral and bacterial infection . Several pre-clinical or phase 1/2 studies [8 9 10 11], have shown decreases in pro-inflammatory cytokines, reduced dendritic cell function, impaired CD4+ and CD8+ T cell priming
In our patient, the asplenia could have potentiated the immune-suppressive effects of ruxolitinib, though the spectrum of atypical infections seen in our patient points to defective cell-mediated immune responses rather than defective humoral responses as is seen in asplenia. Similarly, it is unlikely that hydroxyurea could be responsible for his infections as he had been on hydroxyurea for 20 years prior to starting ruxolitinib without any increase in infections.
The most frequent infections described with ruxolitinib are tuberculosis and Herpes zoster, Hepatitis B virus, cryptococcal, and
Patients receiving ruxolitinib should be counseled regarding the increased risk of infections, and careful monitoring for opportunistic infections is advisable. Future studies on ruxolitinib may benefit from prospective monitoring of CD4, CD8, NK, and B cell profiles of patients for improved understanding of the pathophysiology of the immunodeficiency associated with ruxolitinib.
No potential conflicts of interest relevant to this article were reported.
Trend in total WBC and lymphocyte counts prior to, during and after episode of EBV re-activation.