SitemapContact Us
닫기
Search
Advanced Search +
닫기
Quick links
Most Cited Most Read Ahead of Print Archives Go to E-submission

Original Article

Split Viewer

Blood Res 2015; 50(1):

Published online March 31, 2015

https://doi.org/10.5045/br.2015.50.1.40

© The Korean Society of Hematology

SOCS1 and SOCS3 are expressed in mononuclear cells in human cytomegalovirus viremia after allogeneic hematopoietic stem cell transplantation

Seung-Hwan Shin1,#, Ji Yoon Lee1,3,#, Tae Hyang Lee1,3,#, So-Hye Park1,3, Seung-Ah Yahng1, Jae-Ho Yoon1, Sung-Eun Lee1, Byung-Sik Cho1, Dong-Gun Lee2, Yoo-Jin Kim1, Seok Lee1, Chang-Ki Min1, Seok-Goo Cho1, Dong-Wook Kim1,3, Jong-Wook Lee1, Woo-Sung Min1, Chong-Won Park1, and Hee-Je Kim1,3*

Author Info. +

1Division of Hematology, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

2Division of Infectious diseases, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

3Cancer Research Institute, The Catholic University of Korea, Seoul, Korea.

Correspondence to : Correspondence to Hee-Je Kim, M.D., Ph.D. Division of Hematology, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Cancer Research Institute, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 137-701, Korea. Tel: +82-2-2258-6054, Fax: +82-2-599-3589, cumckim@catholic.ac.kr

Received: October 2, 2014; Revised: November 19, 2014; Accepted: January 27, 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Go to

Background

The expression of the SOCS genes in cytomegalovirus (CMV) viremia after hematopoietic stem cell transplantation (HSCT) remains largely unexplored.

Methods

Using quantitative RT-PCR of mononuclear cells, we conducted pairwise comparison of SOCS1 and SOCS3 expression levels among a healthy donor group (N=55), a pre-HSCT group (N=17), and the recipient subgroup (N=107), which were divided according to the occurrence of CMV viremia and acute graft-versus-host disease (aGVHD).

Results

Compared to that in the healthy donor group, SOCS1 expression was higher in the CMV+ subgroup, especially in the CMV+GVHD- group, but decreased in the other subgroups. When compared to the expression in the pre-HSCT group, SOCS1 expression was significantly higher in the CMV+ subgroup, especially in the CMV+GVHD+ subgroup. Meanwhile, compared to that in the healthy donor group, SOCS3 expression was significantly lower in all other groups. The CMV-GVHD- subgroup showed significantly lower SOCS3 expression compared to the CMV+ subgroup, the CMV+GVHD+ subgroup, and the CMV+GVHD- subgroup.

Conclusion

We report differential expression of SOCS genes according to CMV viremia with acute GVHD occurrence after HSCT, suggesting that regulation of SOCS expression is associated with CMV viremia.

Keywords Suppressor of cytokine signaling (SOCS) 1 and 3, Cytomegalovirus (CMV) viremia, Quantitative real-time PCR, Allogeneic HSCT, GVHD

Article

Original Article

Blood Res 2015; 50(1): 40-45

Published online March 31, 2015 https://doi.org/10.5045/br.2015.50.1.40

Copyright © The Korean Society of Hematology.

SOCS1 and SOCS3 are expressed in mononuclear cells in human cytomegalovirus viremia after allogeneic hematopoietic stem cell transplantation

Seung-Hwan Shin1,#, Ji Yoon Lee1,3,#, Tae Hyang Lee1,3,#, So-Hye Park1,3, Seung-Ah Yahng1, Jae-Ho Yoon1, Sung-Eun Lee1, Byung-Sik Cho1, Dong-Gun Lee2, Yoo-Jin Kim1, Seok Lee1, Chang-Ki Min1, Seok-Goo Cho1, Dong-Wook Kim1,3, Jong-Wook Lee1, Woo-Sung Min1, Chong-Won Park1, and Hee-Je Kim1,3*

1Division of Hematology, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

2Division of Infectious diseases, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

3Cancer Research Institute, The Catholic University of Korea, Seoul, Korea.

Correspondence to: Correspondence to Hee-Je Kim, M.D., Ph.D. Division of Hematology, Department of Internal Medicine, Catholic Blood and Marrow Transplantation Center, Cancer Research Institute, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 137-701, Korea. Tel: +82-2-2258-6054, Fax: +82-2-599-3589, cumckim@catholic.ac.kr

Received: October 2, 2014; Revised: November 19, 2014; Accepted: January 27, 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The expression of the SOCS genes in cytomegalovirus (CMV) viremia after hematopoietic stem cell transplantation (HSCT) remains largely unexplored.

Methods

Using quantitative RT-PCR of mononuclear cells, we conducted pairwise comparison of SOCS1 and SOCS3 expression levels among a healthy donor group (N=55), a pre-HSCT group (N=17), and the recipient subgroup (N=107), which were divided according to the occurrence of CMV viremia and acute graft-versus-host disease (aGVHD).

Results

Compared to that in the healthy donor group, SOCS1 expression was higher in the CMV+ subgroup, especially in the CMV+GVHD- group, but decreased in the other subgroups. When compared to the expression in the pre-HSCT group, SOCS1 expression was significantly higher in the CMV+ subgroup, especially in the CMV+GVHD+ subgroup. Meanwhile, compared to that in the healthy donor group, SOCS3 expression was significantly lower in all other groups. The CMV-GVHD- subgroup showed significantly lower SOCS3 expression compared to the CMV+ subgroup, the CMV+GVHD+ subgroup, and the CMV+GVHD- subgroup.

Conclusion

We report differential expression of SOCS genes according to CMV viremia with acute GVHD occurrence after HSCT, suggesting that regulation of SOCS expression is associated with CMV viremia.

Keywords: Suppressor of cytokine signaling (SOCS) 1 and 3, Cytomegalovirus (CMV) viremia, Quantitative real-time PCR, Allogeneic HSCT, GVHD

Fig 1.

Download
Figure 1.

Comparison of (A)SOCS1 and (B)SOCS3 gene expression levels in the healthy donor group, the pre-HSCT group, and the recipients. Mononuclear cells from recipients were isolated and subjected to real-time quantitative reverse transcription-polymerase chain reaction. Relative gene expression (RE) was normalized to that of ABL1. Error bars indicate±standard error.

Blood Research 2015; 50: 40-45https://doi.org/10.5045/br.2015.50.1.40
Primer and probe sequences used for qRT-PCR.

Abbreviations: SOCS, suppressor of cytokine signaling; ABL1, Abelson murine leukemia viral oncogene homolog 1..
Baseline clinical and transplantation-related characteristics of recipients and donors.

Abbreviations: CMV, cytomegalovirus; AML, acute myelogenous leukemia; ALL, acute lymphoblastic leukemia; MDS, myelodysplastic syndrome; CML, chronic myeloid leukemia; SAA, severe aplastic anemia; BM, bone marrow; PBSC, peripheral blood stem cell; MAC, myeloablative conditioning; RIC, reduced intensity conditioning; ATG, anti-thymocyte globulin; aGVHD, acute graft-versus-host disease..
Expression levels of <italic>SOCS1</italic> and <italic>SOCS3</italic> genes.

Abbreviations: HSCT, hematopoietic stem cell transplantation; CMV, cytomegalovirus; GVHD, graft-versus-host disease..
Blood Res
Volume 59 2024
Current Issue Archives

Stats or Metrics

Share this article on

  • line

Related articles in BR

Blood Research

pISSN 2287-979X
eISSN 2288-0011
qr-code Download