Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.
Allogeneic hematopoietic stem cell transplantation (HSCT) is the preferred curative therapy for children with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). We evaluated the treatment outcomes of children with Ph+ ALL who underwent allogeneic HSCT.
Fifteen children diagnosed with Ph+ ALL in Asan Medical Center Children's Hospital between 1998 and 2012 were retrospectively analyzed.
Of 521 children diagnosed with ALL during the study period, 15 had a Philadelphia chromosome. Among these 15 patients, 13 attained complete remission (CR) following induction chemotherapy, and two died of intracerebral hemorrhage during leukapheresis and induction chemotherapy, respectively. Of the 13 patients who attained CR, 12 received allogeneic HSCT, mainly from unrelated donors. Of the 12 patients who received HSCT, one died of a transplant-related cause, one died of relapse after HSCT, and 10 remain in continuous CR. Of the 10 patients who remained in CR longer than six months after HSCT, seven received post-HSCT imatinib. For all 15 patients, the 5-year overall survival, event-free survival, and cumulative incidence of relapse were 60.0%, 48.6%, and 38.8%, respectively, with a median follow-up of 70 months. For the HSCT group, the 5-year overall survival, event-free survival, and cumulative incidence of relapse were 80.2%, 72.9%, and 29.3%, respectively, with a median follow-up of 100 months.
Allogeneic HSCT cures a significant proportion of Ph+ ALL patients. Because the use of imatinib appears to be a promising approach, strategies that include tyrosine kinase inhibitors before and after HSCT require further evaluation.
Allogeneic hematopoietic stem cell transplantation (HSCT) is the preferred curative therapy for children with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). The Philadelphia chromosome t(9;22)(q34;q11), from which the
The tyrosine kinase inhibitor imatinib mesylate (STI571, Glivec) has been widely used for the treatment of chronic myelogenous leukemia and has recently been used to treat Ph+ ALL . Several studies have shown decreased relapse rates and improved DFS in patients who received imatinib-based treatment prior to allogeneic HSCT compared with those who received conventional allogeneic HSCT . In addition, some studies have shown overall responses with mesylate in 60% to 70% of patients with relapsed or refractory Ph+ ALL, including patients who have previously undergone transplantation, with limited toxicity . Thus, imatinib with conventional chemotherapy before and after HSCT currently appears to be the most curative therapy.
This approach is in agreement with earlier suggestions that successful remission induction of Ph+ ALL requires imatinib to be used in combination with standard chemotherapy. Maintenance therapy with imatinib during the post-transplant period has been used for patients with Ph+ ALL. However, its efficacy compared with that of induction therapy with imatinib during the pre-transplant period remains to be shown. One study has reported reduced relapse rate and improved DFS in Ph+ ALL patients with imatinib maintenance therapy after HSCT .
In our current study, we evaluated the treatment outcomes of children with Ph+ ALL who underwent allogeneic HSCT and examined the feasibility and effect of pre- and post-HSCT imatinib treatment in a single center.
This study analyzed patients diagnosed with Ph+ ALL at Asan Medical Center Children's Hospital (AMCCH) in Seoul, Korea between 1998 and 2012. For all surviving patients, the end point of the last follow-up was April 30, 2013. ALL was diagnosed according to conventional criteria. Ph+ ALL was diagnosed by the presence of the Philadelphia chromosome t(9;22)(q34;q11.2) through metaphase cytogenetics, fluorescence in situ hybridization (FISH) analysis, and by positivity for
Patient-, disease-, transplantation-, and imatinib treatment-related variables were expressed as median and range, number, or percentage, as appropriate. We assessed the effects of treatment using OS and event-free survival (EFS). OS and EFS were calculated using the Kaplan-Meier method. Statistical analyses were performed using SPSS for Windows, version 21.0 (IBM Corp., Armonk, NY).
A total of 15 children (2.9%) were diagnosed with Ph+ ALL and 521 children were diagnosed with ALL at AMCCH during the study period. Patient characteristics are listed in Table 1. Ten patients were male (67%) and 5 were female (33%). The median patient age at diagnosis was 9.6 years (range, 3.1-15.8 years) and the median white blood cell count was 30,000/mm3 (range, 6,200-701,400/mm3). No patients had central nervous system involvement, although the central nervous system involvement of one patient was not evaluated due to death from multifocal intracranial hemorrhage during induction chemotherapy on leukapheresis. Immunophenotypic analysis showed that all patients had B-cell precursor ALL. Eleven patients had minor
Of the 15 Ph+ ALL patients in our current series, two died during induction chemotherapy. One of these patients had an initial peripheral white blood cell count at diagnosis of 701,400/mm3 and developed intracranial hemorrhage on leukapheresis. The other had tumor lysis syndrome and also developed intracranial hemorrhage on hemodialysis. Thirteen patients, excluding the above-mentioned cases, achieved hematologic complete remission (CR) after induction chemotherapy. One subsequently died of relapse and disease progression despite achieving a second CR following imatinib treatment. Consequently, 12 patients received allogeneic HSCT. Among these 12 cases, the median age at HSCT was 9.3 years (range, 3.1-15.8 years) and the median time to HSCT after diagnosis was 5.8 months (range, 3.2-6.9 months). All 12 patients achieved hematologic remission before HSCT, although one relapsed before HSCT and achieved a second hematologic remission following a second induction chemotherapy regimen. Ten patients achieved cytogenetic remission, one still had the t(9:22) chromosome, and there were no chromosomal data before HSCT for one case. Seven patients achieved molecular remission, three patients did not, and there were no
Of the 12 patients in our cohort who underwent HSCT, two relapsed after treatment. One subsequently died of disease progression; the other had an extramedullary relapsed lesion and was alive without disease after additional chemotherapy. There was one case of transplant-related mortality (TRM) involving a patient who died of pulmonary hemorrhage at post-transplantation day 8. The other 10 cases remained in continuous CR. Of 11 evaluable patients, six had acute GVHD: three grade I-II, two grade III, and one grade IV; three had chronic GVHD: two of limited disease and one of extensive disease. Table 3 lists the HSCT outcomes of the 12 patients analyzed in this study.
Table 4 lists diagnostic information for each patient as well as the HSCT and imatinib treatment outcomes in the 12 study patients who underwent HSCT. Ten of these cases received pre-HSCT imatinib treatment: two underwent imatinib during induction and consolidation chemotherapy and eight received imatinib only during consolidation chemotherapy. The median duration of imatinib treatment until HSCT was 2.5 months (range, 1.3-4.2 months) and the median imatinib dosage was 332 mg/m2 (range, 243-400 mg/m2). Among our 12 patients, seven received post-transplant imatinib treatment and two did not due to early TRM and relapse. Of the 10 patients who remained in CR for longer than six months after HSCT, seven were started on imatinib at a median of 6.3 months after HSCT (range, 2.9-9.6 months) and continued this treatment for a median of 4.8 months (range, 4.2-8.4 months), with a median imatinib dose of 213 mg/m2 (range, 105-353 mg/m2). Four of the 10 patients who received pre- or post-HSCT imatinib treatment underwent imatinib dose reduction because of grade 1 neutropenia, grade 3 febrile neutropenia, grade 1 impaired liver function, and grade 1 skin eruption (one case each). All patients who received post-HSCT imatinib treatment were alive at the time of writing.
For the 15 study patients, the 5-year OS was 60.0% (Standard error [SE], 14.2%) and the EFS was 48.6% (SE, 14.1%), with a median follow-up of 70 months (Fig. 1). For the specific HSCT group (N=12), the 5-year OS and EFS were 80.2% (SE, 12.8%) and 72.9% (SE, 13.6%), respectively, with a median follow-up of 100 months (Fig. 2). Seven patients who received pre- and post-HSCT imatinib treatment were alive without relapse.
Treatment outcomes for both Ph+ ALL and ALL patients have improved in recent years due to improvements in chemotherapy and HSCT methods. In our current study, the 5-year OS and EFS of Ph+ ALL patients were higher than previously reported : of 12 patients who received HSCT, 10 were still living at the time of writing-one patient died of TRM and one died of the disease-resulting in 5-year OS and EFS rates of 80.2% and 72.9%, respectively, with a median follow-up of 100 months. These results suggest that allogeneic HSCT may be a curative option for a significant portion of patients with Ph+ ALL.
Although previous studies have reported that patients who underwent HSCT from a matched related donor had a significantly lower risk of treatment failure , the 5-year OS and EFS results for alternative donors in our study were quite favorable. Among the donors for our 12 HSCT patients, nine (75%) were unrelated and the other three consisted of a haploidentical, an HLA-DR mismatched sibling, and a matched sibling donor. Thus, HSCT using alternative donors can be considered for patients without matched family donors.
The International Childhood Acute Lymphoblastic Leukemia Study Group  reported a 5-year OS and EFS of 40±3% and 28±3%, respectively, for 326 Ph+ ALL patients diagnosed between 1986 and 1996 and 5-year OS and EFS of 72±8% and 65±8%, respectively, for 38 patients treated with HSCT from HLA-matched related donors among 267 Ph+ ALL patients who achieved CR after induction chemotherapy. These previous studies also reported a 7-year OS and EFS of 32±2% and 44.9±2.2% for 610 Ph+ ALL patients treated between 1995 and 2005, respectively, and 5-year OS and EFS of 54±2.8% and 43.5±2.9% for 325 patients who underwent HSCT with different types of donors among 542 Ph+ ALL patients who achieved remission by the end of induction therapy . The King Hussein Cancer Center has reported a 5-year OS and EFS of 91.6±7.9% and 75±12.5% for 12 Ph+ ALL childhood patients diagnosed from 2003 until 2011 who underwent HSCT, with a median follow-up of 42.2 months . Although there is a controversy surrounding the best treatment for childhood Ph+ ALL patients, the results of our study and previous reports suggest that HSCT, especially with matched sibling donors, appears to be the best treatment option. The treatment results for childhood Ph+ ALL cases have improved over time with advances in chemotherapy and HSCT methods and decreased incidence of complications. Our current results indicate acceptable 5-year OS and EFS, despite our inclusion of patients who were diagnosed in the late 1990s and the predominant use of unrelated donors in this series.
Although the overall outcomes for children with ALL have continued to improve, the outcomes for the specific Ph+ ALL subgroup have not.
In contrast to the aforementioned findings, the overall effect of post-transplant imatinib administration on the outcomes of allogeneic HSCT remains undetermined. Several studies have reported the safety and efficacy of imatinib therapy after allogeneic HSCT. One study reported favorable 5-year OS and DFS (81.5±5% and 86.7±4.4%, respectively) in 62 Ph+ ALL patients receiving a post-HSCT imatinib treatment of 400 mg/day for adults (>17 years) and 260 mg/m2/day for children (<17 years) . Their median age was 29 years (range, 6-50 years), and they had received imatinib for a median of 70 days post-HSCT (range, 20-270 days) with a median duration of 90 days (range, 13-540 days). Another related study reported a 3-year OS of 66.7% with post-HSCT imatinib administration, but there was no significant benefit found in terms of EFS compared with the post-HSCT non-imatinib group . We administered a relatively small amount of imatinib over a longer period in our current study, and all patients who received post-HSCT imatinib are still living without relapse or disease-associated morbidity. Because of the small number of patients, we could not directly compare outcomes between the post-HSCT imatinib and no post-HSCT imatinib treatment groups in our study. However, post-HSCT imatinib treatment appeared to be a feasible option, with 100% OS in the post-HSCT imatinib group.
In 2009, the Children's Oncology Group (COG) reported that imatinib plus intensive chemotherapy improves the 3-year EFS in children and adolescents with Ph+ ALL with no appreciable increase in toxicity, but that HSCT plus imatinib offered no advantage over HSCT alone . The related donor HSCT group in the COG study treated with 6 months of imatinib post-HSCT had a 3-year EFS of 56.6±21.5%, which was similar to that of a comparable historical HSCT group from the CCG-1921 study . Recently, the continuing COG study AALL0031, which is evaluating the 5-year outcome of imatinib plus intensive chemotherapy with and without allogeneic HSCT, reported a 5-year EFS for the imatinib plus intensive chemotherapy group of 70±12%, compared with 65±11% for related donor HSCT patients and 59±15% for unrelated donor HSCT patients . This result suggests that imatinib plus intensive chemotherapy may be the best initial treatment of choice for Ph+ ALL children. However, there are insufficient long-term follow-up data at present to fully validate this possibility and it is still unclear when imatinib should be used. Longer-term outcomes and specific strategies for imatinib need to be studied to properly elucidate the effect of imatinib plus intensive chemotherapy and possible benefit of imatinib plus intensive chemotherapy over HSCT. In comparison, our results for imatinib plus HSCT are comparable with those of the COG study and showed a low TRM and GVHD rate, even though nearly all of our HSCT donors were unrelated. Thus, our findings suggest that allogeneic HSCT still plays an important role in the treatment of patients with Ph+ ALL.
Many previous studies have additionally investigated the effect of other tyrosine kinase inhibitors besides imatinib, including dasatinib and nilotinib. More studies are necessary to increase the efficacy and decrease the toxicity of these agents.
Despite our findings of favorable outcomes of allogeneic HSCT, this study had some limitations. These included the small number of patients analyzed and the long study period during which changes were made to the Ph+ ALL treatment regimen. Additionally, this study had a short follow-up duration, especially in the imatinib treatment group (median, 50 months).
In conclusion, the 5-year OS and EFS of patients with Ph+ ALL who received allogeneic HSCT are favorable with pre- and post-transplant administration of imatinib. Allogeneic HSCT with pre- and post-transplant maintenance using a tyrosine kinase inhibitor may thus be a curative option for Ph+ ALL. The outcomes of imatinib plus intensive chemotherapy without allogeneic HSCT should be compared with those of allogeneic HSCT in further studies. In addition, the dose and duration of pre- and post-transplant imatinib should be evaluated accurately to clarify the role of imatinib in childhood Ph+ ALL.