Korean J Hematol 1998; 33(1):

Published online March 31, 1998

© The Korean Society of Hematology

급성 백혈병에서의 pl6 및 pRb의 발현 양상

이정림, 이주희, 서진태, 이우인, 이희주

경희대학교 의과대학 임상병리과학교실,
경희대학교 의과대학 해부병리과학교실

Pattern of pl6 and pRb Expression in Acute Leukemia

Jeong Rim Lee, Ju Hee Lee, Jin Tae Suh, Wu In Lee, Hee Jue Lee

Department of Clinical Pathology, Anatomical Pathology, College of Medicine, Kyung Hee University, Seoul, Korea

Abstract

Background: There have been evidences that inactivation of tumor supressor genes such as p16 and pRb develops human malignancies. p16 inhibits the cyclin D/cyclin-dependant kinase 4 complex which phosphorylates pub, thus negatively regulating cell cycle progression. pRb gene inactivation has been observed in various tumor and reported to be reciprocally correlated with p16 expression. The purpose of this study is to evaluate aberrant expression of p16 and pRb in acute leukemia by
immunohistochemical stain in clot or biopsy section of the bone marrow.
Methods: For detection of p16 and pRb, immunohistochemical staining with anti-human mouse monoclonal antibodies to p16 and pRb were done on 62 bone marrow parameters sections from leukemic patients. In acute lymphoblastic leukemia(ALL), immunophenotyping was performed with indirect immunofluorescent assay and clinical parameters were analyzed depending on status of p16 expression.
Results: In 28 cases(45%), abnormal expression of p16 or pRb were observed. Twenty three of those cases(37%) showed aberrancy only in one protein, in which case, staining intensity was generally more stronger than that of normal expression in both proteins. The proportion of abnormal p16 expression was 30% and 15%, and that of pRb was 30% and 36% in ALL and in acute myelocytic leukemia, respectively. There was no significant clinical correlation between aberrancy of p16 and clinical parameters of acute leukemia.
Conclusion: Our results suggest that loss of the p16 and pRb function may contribute to pathogenesis of acute leukemia. Further study is needed in more cases of acute
leukemia to evaluate clinical significance of p16 or pRb aberrancy, particularly in ALL.

Keywords p16; pRb; Acute leukemia;

Article

Korean J Hematol 1998; 33(1): 80-93

Published online March 31, 1998

Copyright © The Korean Society of Hematology.

급성 백혈병에서의 pl6 및 pRb의 발현 양상

이정림, 이주희, 서진태, 이우인, 이희주

경희대학교 의과대학 임상병리과학교실,
경희대학교 의과대학 해부병리과학교실

Pattern of pl6 and pRb Expression in Acute Leukemia

Jeong Rim Lee, Ju Hee Lee, Jin Tae Suh, Wu In Lee, Hee Jue Lee

Department of Clinical Pathology, Anatomical Pathology, College of Medicine, Kyung Hee University, Seoul, Korea

Abstract

Background: There have been evidences that inactivation of tumor supressor genes such as p16 and pRb develops human malignancies. p16 inhibits the cyclin D/cyclin-dependant kinase 4 complex which phosphorylates pub, thus negatively regulating cell cycle progression. pRb gene inactivation has been observed in various tumor and reported to be reciprocally correlated with p16 expression. The purpose of this study is to evaluate aberrant expression of p16 and pRb in acute leukemia by
immunohistochemical stain in clot or biopsy section of the bone marrow.
Methods: For detection of p16 and pRb, immunohistochemical staining with anti-human mouse monoclonal antibodies to p16 and pRb were done on 62 bone marrow parameters sections from leukemic patients. In acute lymphoblastic leukemia(ALL), immunophenotyping was performed with indirect immunofluorescent assay and clinical parameters were analyzed depending on status of p16 expression.
Results: In 28 cases(45%), abnormal expression of p16 or pRb were observed. Twenty three of those cases(37%) showed aberrancy only in one protein, in which case, staining intensity was generally more stronger than that of normal expression in both proteins. The proportion of abnormal p16 expression was 30% and 15%, and that of pRb was 30% and 36% in ALL and in acute myelocytic leukemia, respectively. There was no significant clinical correlation between aberrancy of p16 and clinical parameters of acute leukemia.
Conclusion: Our results suggest that loss of the p16 and pRb function may contribute to pathogenesis of acute leukemia. Further study is needed in more cases of acute
leukemia to evaluate clinical significance of p16 or pRb aberrancy, particularly in ALL.

Keywords: p16, pRb, Acute leukemia,

Blood Res
Volume 59 2024

Stats or Metrics

Share this article on

  • line

Blood Research

pISSN 2287-979X
eISSN 2288-0011
qr-code Download