Korean J Hematol 1997; 32(2):
Published online June 30, 1997
© The Korean Society of Hematology
골수이형성증에서 아포토시스 유무와 Fas(CD95)항원의 표현 및 그 임상적 의의
유병우, 한기주, 목지오, 전진우, 박성규, 원종호, 백승호, 서원석, 홍대식, 이동화, 박희숙
순천향대학교 의과대학 내과학교실,
순천향대학교 의과대학 임상분자생물학연구소,
순천향대학교 의과대학 소아과학교실,
순천향대학교 의과대학 임상병리학교실
The Expression of Fas Antigen and Apoptosis in Patients with Myelodysprastic Syndromes and Its Clinical Relevance
Background: The myelodysplastic syndromes(MDS) are a group of acquired clonal hematopoietic disorders characterized by the peripheral cytopenias and hypercellular or normocellular dysplastic bone marrow. The event responsible for the development of MDS is generally not blown. Several recent reports have described an increased frequency of apoptosis in bone marrow cells from patients with aplastic anemia or MDS. Gersuk et al observed that Fas ligand expression was significantly increased on bone marrow cells from MDS patients as compared to normal individuals.
Methods: We examined apoptosis and Fas antigen expression using ISNEL method and immunohistochemistry on marrow cells from 36 patients with MDS.
Results: Among the 36 patients, 15 patients(42%) demonstrated apoptosis positive cells(>15%) and 9 patients(25%) demonstrated positive Fas antigen expression(>20%). The presence of apoptosis significantly correlated with hemoglobin level at diagnosis(P<0.05) and the expression of Fas antigen significantly correlated with bone marrow cellularity and CD34 positive cell aggregate group at diagnosis(P<0.05). There was no statistically significant relationship between apoptosis and Fas antigen expression. The median survival of all patients with MDS was 44 months(2-117+). Multivariate analysis showed that FAB classification and hemoglobin level at diagnosis were
significant prognostic factor but presence of apoptosis and expression of Fas antigen had no significance.
Conclusion: The data indicate that the apoptosis and expression of Fas antigen are present in patients with MDS and correlate with some clinical parameters but not
significantly associated with survival period of the patients with MDS.
Keywords Myelodysplastic syndrome; Apoptosis; Fas antigen; Prognostic factor;
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Korean J Hematol 1997; 32(2): 256-265
Published online June 30, 1997
Copyright © The Korean Society of Hematology.
골수이형성증에서 아포토시스 유무와 Fas(CD95)항원의 표현 및 그 임상적 의의
유병우, 한기주, 목지오, 전진우, 박성규, 원종호, 백승호, 서원석, 홍대식, 이동화, 박희숙
순천향대학교 의과대학 내과학교실,
순천향대학교 의과대학 임상분자생물학연구소,
순천향대학교 의과대학 소아과학교실,
순천향대학교 의과대학 임상병리학교실
The Expression of Fas Antigen and Apoptosis in Patients with Myelodysprastic Syndromes and Its Clinical Relevance
Byung-Woo Yu, Kie, Ju Han, Ji, Oh Mok, Jin, Woo Jeon, Sung, Kyu Park, Jong, Ho Won, Seung, Ho Baick, Won, Suk Suh, Dae, Sik Hong, Dong, Wha Lee, Hee, Sook Park
Department of Internal Medicine, Pediatrics, Clinical Pathology, Soonchunghyang University College of Medicine, Seoul, Korea
Department of Institute for Clinical Molecular Biology Research, Korea
Abstract
Background: The myelodysplastic syndromes(MDS) are a group of acquired clonal hematopoietic disorders characterized by the peripheral cytopenias and hypercellular or normocellular dysplastic bone marrow. The event responsible for the development of MDS is generally not blown. Several recent reports have described an increased frequency of apoptosis in bone marrow cells from patients with aplastic anemia or MDS. Gersuk et al observed that Fas ligand expression was significantly increased on bone marrow cells from MDS patients as compared to normal individuals.
Methods: We examined apoptosis and Fas antigen expression using ISNEL method and immunohistochemistry on marrow cells from 36 patients with MDS.
Results: Among the 36 patients, 15 patients(42%) demonstrated apoptosis positive cells(>15%) and 9 patients(25%) demonstrated positive Fas antigen expression(>20%). The presence of apoptosis significantly correlated with hemoglobin level at diagnosis(P<0.05) and the expression of Fas antigen significantly correlated with bone marrow cellularity and CD34 positive cell aggregate group at diagnosis(P<0.05). There was no statistically significant relationship between apoptosis and Fas antigen expression. The median survival of all patients with MDS was 44 months(2-117+). Multivariate analysis showed that FAB classification and hemoglobin level at diagnosis were
significant prognostic factor but presence of apoptosis and expression of Fas antigen had no significance.
Conclusion: The data indicate that the apoptosis and expression of Fas antigen are present in patients with MDS and correlate with some clinical parameters but not
significantly associated with survival period of the patients with MDS.
Keywords: Myelodysplastic syndrome, Apoptosis, Fas antigen, Prognostic factor,
