Korean J Hematol 1991; 26(2):
Published online June 30, 1991
© The Korean Society of Hematology
윤종현, 심명옥, 송운흥, 조한익, 김상인
서울대학교 의과대학 임상병리학교실
We introduced microwave stimulated method for peroxidase, sudan black B(SBB), acid
phospha-tase, non-specific esterase and periodic acid-Schiff(PAS) stain for bone marrow
aspiration smears and Romanowsky-Giemsa stain of plastic embbedded bone marrow
section to reduce time. The microwave method reduced reaction times from 7-60
minutes of conventional methods to 2-10 minutes, that resulted in 24-77% reduction of
whole staining time. The staining intensity and the quality are almost equal to those of
conventional methods. We accepted microwave stimulated method as our routine
procedures of cytochemical stainings of bone marrow aspiration smears and plastic
embedded bone marrow section.
Keywords Microwave, Bone marrow, Staining
Korean J Hematol 1991; 26(2): 357-362
Published online June 30, 1991
Copyright © The Korean Society of Hematology.
윤종현, 심명옥, 송운흥, 조한익, 김상인
서울대학교 의과대학 임상병리학교실
Jong Hyun Yoon, Myong Ok Shim, Woon Heung Song, Han It Cho, Sang In Kim
Department of Clinical Pathology, Seoul National University College of Medicine, Seoul Korea
We introduced microwave stimulated method for peroxidase, sudan black B(SBB), acid
phospha-tase, non-specific esterase and periodic acid-Schiff(PAS) stain for bone marrow
aspiration smears and Romanowsky-Giemsa stain of plastic embbedded bone marrow
section to reduce time. The microwave method reduced reaction times from 7-60
minutes of conventional methods to 2-10 minutes, that resulted in 24-77% reduction of
whole staining time. The staining intensity and the quality are almost equal to those of
conventional methods. We accepted microwave stimulated method as our routine
procedures of cytochemical stainings of bone marrow aspiration smears and plastic
embedded bone marrow section.
Keywords: Microwave, Bone marrow, Staining
Hye Won Lee and Ja Young Lee
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