Korean J Hematol 2004; 39(4):
Published online December 31, 2004
© The Korean Society of Hematology
김대훈, 최병규, 홍승아, 김흥식, 강진무, 박종욱, 하정숙, 류남희, 김재룡, 권건영
계명대학교 의과대학 소아과학교실
, 계명대학교 의과대학 면역학교실
, 계명대학교 의과대학 진단검사의학교실
, 계명대학교 의과대학 해부병리학교실
Background :
Cell lines can be established when the cells are clonally selected and propagated continuously in vitro culture system. Recently we established a B cell line (KEB1) from the bone marrow cells of the patient infected with Epstein-Barr virus (EBV).
Methods :
The patient´s initial platelet count was 1,000/microL and peripheral blood smear showed atypical lymphocytes accounting 20% of the differentials of WBC. Antibodies to EBV and PCR for EBV were positive but heterophil antibody was negative. Mononuclear cells were obtained by Ficoll-paque separation and suspended in RPMI media with 10% FCS. After incubation in 37degrees C, 5% CO2 incubator, cells grew continuously and finally immortalized to B cell line.
Results :
Cells showed abundant, clear basophilic cytoplasms and a few vacuoles. Cells had granular reaction in PAS stain and were positive to B cell antibodies. Immunohistochemical stain showed positive expression for EBV antibody. Electron microscopic finding of cultured cells showed several viral particles, and immunoelectron microscopic finding showed electron dense expression. Immunophenotyping of cultured cells was positive for B lymphoid lineage, and karyotypings had hypotetraploidy. Cells expressed MAGE and SSX gene. Cytotoxicity showed relative resistance to mistletoe and several chemotherapeutic agents compared to leukemic cell line.
Conclusion :
KEB1 cell line was established from the bone marrow cells of the patient with infectious mononucleosis. The characteristics of the cell lines including morphology, immunophenotype, karyotype, gene analysis (MAGE, SSX) and chemosensitivity were analyzed. There should be further studies of these cell lines including gene analysis, telomerase activity andcytokine production. This cell line might be helpful to establish another normal lymphocyte cell line and to predict the toxicity of chemotherapy.
Keywords Cell Line, Bone marrow, Epstein-Barr virus, Chemosensitivity
Korean J Hematol 2004; 39(4): 233-242
Published online December 31, 2004
Copyright © The Korean Society of Hematology.
김대훈, 최병규, 홍승아, 김흥식, 강진무, 박종욱, 하정숙, 류남희, 김재룡, 권건영
계명대학교 의과대학 소아과학교실
, 계명대학교 의과대학 면역학교실
, 계명대학교 의과대학 진단검사의학교실
, 계명대학교 의과대학 해부병리학교실
Kim DH, Choe BK, Hong SA, Kim HS, Kang CM, Park JW, Ha JS, Ryoo NH, Kim JR, Kwon KY
Department of Pediatrics, Keimyung University School of Medicine, Daegu, Korea,
Department of Immunology, Korea,
Department of Laboratory Medicine, Korea
, Department of Pathology, Korea.
Background :
Cell lines can be established when the cells are clonally selected and propagated continuously in vitro culture system. Recently we established a B cell line (KEB1) from the bone marrow cells of the patient infected with Epstein-Barr virus (EBV).
Methods :
The patient´s initial platelet count was 1,000/microL and peripheral blood smear showed atypical lymphocytes accounting 20% of the differentials of WBC. Antibodies to EBV and PCR for EBV were positive but heterophil antibody was negative. Mononuclear cells were obtained by Ficoll-paque separation and suspended in RPMI media with 10% FCS. After incubation in 37degrees C, 5% CO2 incubator, cells grew continuously and finally immortalized to B cell line.
Results :
Cells showed abundant, clear basophilic cytoplasms and a few vacuoles. Cells had granular reaction in PAS stain and were positive to B cell antibodies. Immunohistochemical stain showed positive expression for EBV antibody. Electron microscopic finding of cultured cells showed several viral particles, and immunoelectron microscopic finding showed electron dense expression. Immunophenotyping of cultured cells was positive for B lymphoid lineage, and karyotypings had hypotetraploidy. Cells expressed MAGE and SSX gene. Cytotoxicity showed relative resistance to mistletoe and several chemotherapeutic agents compared to leukemic cell line.
Conclusion :
KEB1 cell line was established from the bone marrow cells of the patient with infectious mononucleosis. The characteristics of the cell lines including morphology, immunophenotype, karyotype, gene analysis (MAGE, SSX) and chemosensitivity were analyzed. There should be further studies of these cell lines including gene analysis, telomerase activity andcytokine production. This cell line might be helpful to establish another normal lymphocyte cell line and to predict the toxicity of chemotherapy.
Keywords: Cell Line, Bone marrow, Epstein-Barr virus, Chemosensitivity
Hye Won Lee and Ja Young Lee
Blood Res 2024; 59():Željka Tkalčić Švabek, Marina Josipović, Ivana Horvat, Renata Zadro, and Sanja Davidović-Mrsić
Blood Res 2018; 53(2): 152-159Hyun-Young Kim, Mi-Ae Jang, Hee-Jin Kim, Seok Jin Kim, Won Seog Kim, and Sun-Hee Kim
Blood Res 2017; 52(3): 193-199