Korean J Hematol 1998; 33(2):
Published online June 30, 1998
© The Korean Society of Hematology
인체 제대혈 내의 조혈세포 양 및 HLA 분포: 제대혈은행 설립을 위한 기초 조사
김상희, 김태원, 김신, 박정선, 이제환, 김성배, 서철원, 이규형, 이정신, 김우건, 이인식, 김암, 서종진, 지현숙
울산대학교 의과대학 서울중앙병원 종양혈액내과,
울산대학교 의과대학 서울중앙병원 산부인과,
울산대학교 의과대학 서울중앙병원 임상병리과
The Amount of Hematopoietic Stem Cell and the Distribution of HLA in Human Cord Blood: Basic Study for the Development of Cord Blood Bank
Background: Because there are lower incidence of graft versus host disease in HLA mismatched cord blood transplantation compared to bone marrow transplantation,
development of smaller scale cord blood bank could be possible. So we analysed the content of hematopoietic stem cell in cord blood and the distribution of HLA as a basic
study for cord blood bank.
Methods: Seventy eight cord bloods were collected in heparinized bottle immediately after caesarian section. After expulsion of placenta, additional cord blood and placental blood were collected with heparinized syringe. Fifteen mL was sent to the laboratory for analysis and the rest was cryopreserved.
Results: The mean collected cord blood volume was 96.8㎖(range, 55∼163㎖). And mean 81.8㎖(range, 40∼148㎖) was cryopreserved. It contained mean 7.4×10 8(range, 2.8 × 10 8~12.2×10 8) nucleated cells.
In 2×10 5 mononuclear cells, 85±48 BFU-E, 19±17 CFU-E, 107±73 CFU-GM and 124±113 CFU-GEMM were present. With dextran/albumin thawing media, the viability of cryopreserved cord blood mononuclear cell was better than usual washing method with IMDM(82.3% vs. 74.6% P=0.004). Each cord blood could find HLA full matched, 5 loci matched and 4 loci matched cord blood in the remainders with the probability of 0, 11.9% and 58.4%.
Conclusion: Development of more smaller scale cord blood bank could be possible compared to bone marrow bank.
Keywords Cord blood bank; HLA; Hematopoietic stem cell;
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Korean J Hematol 1998; 33(2): 171-176
Published online June 30, 1998
Copyright © The Korean Society of Hematology.
인체 제대혈 내의 조혈세포 양 및 HLA 분포: 제대혈은행 설립을 위한 기초 조사
김상희, 김태원, 김신, 박정선, 이제환, 김성배, 서철원, 이규형, 이정신, 김우건, 이인식, 김암, 서종진, 지현숙
울산대학교 의과대학 서울중앙병원 종양혈액내과,
울산대학교 의과대학 서울중앙병원 산부인과,
울산대학교 의과대학 서울중앙병원 임상병리과
The Amount of Hematopoietic Stem Cell and the Distribution of HLA in Human Cord Blood: Basic Study for the Development of Cord Blood Bank
Sang Hee Kim, Tae Won Kim, Shin Kim, Jeong Sun Park, Je Hwan Lee, Sung Bae Kim, Cheolwon Suh, Kyoo Hyung Lee, Jung Shin Lee, Woo Kun Kim, In Sik Lee, Ahm Kim, Jong Jin Seo, Hyun Sook Chi
Department of Medicine, Obstetrics and Gynecology, Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea
Abstract
Background: Because there are lower incidence of graft versus host disease in HLA mismatched cord blood transplantation compared to bone marrow transplantation,
development of smaller scale cord blood bank could be possible. So we analysed the content of hematopoietic stem cell in cord blood and the distribution of HLA as a basic
study for cord blood bank.
Methods: Seventy eight cord bloods were collected in heparinized bottle immediately after caesarian section. After expulsion of placenta, additional cord blood and placental blood were collected with heparinized syringe. Fifteen mL was sent to the laboratory for analysis and the rest was cryopreserved.
Results: The mean collected cord blood volume was 96.8㎖(range, 55∼163㎖). And mean 81.8㎖(range, 40∼148㎖) was cryopreserved. It contained mean 7.4×10 8(range, 2.8 × 10 8~12.2×10 8) nucleated cells.
In 2×10 5 mononuclear cells, 85±48 BFU-E, 19±17 CFU-E, 107±73 CFU-GM and 124±113 CFU-GEMM were present. With dextran/albumin thawing media, the viability of cryopreserved cord blood mononuclear cell was better than usual washing method with IMDM(82.3% vs. 74.6% P=0.004). Each cord blood could find HLA full matched, 5 loci matched and 4 loci matched cord blood in the remainders with the probability of 0, 11.9% and 58.4%.
Conclusion: Development of more smaller scale cord blood bank could be possible compared to bone marrow bank.
Keywords: Cord blood bank, HLA, Hematopoietic stem cell,
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