Korean J Hematol 1997; 32(2):
Published online June 30, 1997
© The Korean Society of Hematology
설미영, 이선경, 서강석, 이은엽, 김순호
부산대학교 의과대학 병리학교실,
부산대학교 의과대학 임상병리학교실
Background: Non-Hodgkin lymphomas have morphologic and biologic variabilities and are classified by a variety of classification schemes. The authors studied the value of
DNA content, S-phase fraction, and nuclear areas as a diagnostic discriminant in classification and predicting the clinical outcome of non-Hodgkin lymphoma.
Methods: Thirty cases of non-Hodgkin lymphoma and 13 cases of reactive lymph node specimens embedded in paraffin were used. DNA analysis was done by flow cytometric method using propidium iodide staining. Nuclear areas of lymphoma cells were measured by image analyzer. The results of DNA content, S-phase fraction, nuclear areas were statistically analyzed between groups of lymphoma classified according to the Rappaport classification and Working formulation.
Results : Aneuploidy patterns were identified in two cases(6.7%) of non-Hodgkin lymphoma(diffuse poorly differentiated lymphocytic and well differentiated lymphocytic). S-phase fraction and nuclear areas were high in lymphoblastic, diffuse poorly differentiated, diffuse lymphocytic undifferentiated, and diffuse mixed types(unfavorable prognostic group), and which had statistical significance against the low values of diffuse lymphocytic well differentiated type(favorable prognostic group). The S-phase fraction and nuclear area had significant correlation, so they could represent correlation between high proliferative activity and large nuclear area. High, intermediate, and low grades groups classified by Working formulation revealed statistically significant differences of S-phase fraction and nuclear areas between each group.
Conclusion: The S-phase fraction and nuclear area measurement could be good diagnostic and prognostic factors in classification and clinical management of
non-Hodgkin lymphoma.
Keywords Non-Hodgkin lymphoma; DNA content; S-phase fraction; Nuclear area;
Korean J Hematol 1997; 32(2): 266-274
Published online June 30, 1997
Copyright © The Korean Society of Hematology.
설미영, 이선경, 서강석, 이은엽, 김순호
부산대학교 의과대학 병리학교실,
부산대학교 의과대학 임상병리학교실
Mee Young Sol, Sun Kyung Lee, Kang Suek Suh, Eun Yup Lee, Soon Ho Kim
Department of Pathology,Clinical Pathology, College of Medicine, Pusan National University, Pusan, Korea
Background: Non-Hodgkin lymphomas have morphologic and biologic variabilities and are classified by a variety of classification schemes. The authors studied the value of
DNA content, S-phase fraction, and nuclear areas as a diagnostic discriminant in classification and predicting the clinical outcome of non-Hodgkin lymphoma.
Methods: Thirty cases of non-Hodgkin lymphoma and 13 cases of reactive lymph node specimens embedded in paraffin were used. DNA analysis was done by flow cytometric method using propidium iodide staining. Nuclear areas of lymphoma cells were measured by image analyzer. The results of DNA content, S-phase fraction, nuclear areas were statistically analyzed between groups of lymphoma classified according to the Rappaport classification and Working formulation.
Results : Aneuploidy patterns were identified in two cases(6.7%) of non-Hodgkin lymphoma(diffuse poorly differentiated lymphocytic and well differentiated lymphocytic). S-phase fraction and nuclear areas were high in lymphoblastic, diffuse poorly differentiated, diffuse lymphocytic undifferentiated, and diffuse mixed types(unfavorable prognostic group), and which had statistical significance against the low values of diffuse lymphocytic well differentiated type(favorable prognostic group). The S-phase fraction and nuclear area had significant correlation, so they could represent correlation between high proliferative activity and large nuclear area. High, intermediate, and low grades groups classified by Working formulation revealed statistically significant differences of S-phase fraction and nuclear areas between each group.
Conclusion: The S-phase fraction and nuclear area measurement could be good diagnostic and prognostic factors in classification and clinical management of
non-Hodgkin lymphoma.
Keywords: Non-Hodgkin lymphoma, DNA content, S-phase fraction, Nuclear area,