Korean J Hematol 1997; 32(1):
Published online March 31, 1997
© The Korean Society of Hematology
급성 백혈병에서 면역학적 표지자의 표현양상
최현식, 김기연, 이중원, 서장수, 이원길, 김재식, 전동석
경북대학교 의과대학 임상병리학교실,
계명대학교 의과대학 임상병리학교실
Expression Patterns of Immunologic Surface Markers in Acute Leukemia
Background: Immunophenotyping is an important technique for the diagnosis and classification of acute leukemia, as well as French-American-British(FAB) classification
on the basis of morphologic characteristics and cytochemistry. We evaluated the expression patterns of immunologic surface markers in acute leukemia.
Methods: Peripheral or bone marrow leukemic cells from 75 leukemic patients(acute lymphoblastic leukemia, ALL 40 cases; children(26 cases), adults(14 cases) and acute
myeloid leukemia, AML 35 cases; children(9 cases), adults(26 cases)) were studied. Monoclonal antibodies which were designed for two color direct immunofluorescence(IF)
analysis with combination of fluoresceinisothiocynate(FITC) and phycoerythrin(PE) conjugated, CD10/CD19, CD20/CD5, CD3/CD22, CD7/CD33, HLA-DR/CD 13(Acute Leukemia Phenotyping Kit, Beckon Dickinson; BD, USA) were analyzed by flow
cytometry.
Results: Blasts from these patients could be classified as CALLA(+)B-ALL(26 cases, 65.0%), CALLA(-)B-ALL(6 cases, 15.0%), T-ALL(6 cases, 15.0%), biphenotypic ALL(2 cases, 5.0%). The positive expression rates were CDl9(100%), CD10(78.1%), CD22(75.0%) and CD20(50.0%) in B-ALL, CD7(100%), CD3(50.0%) and CD5(50.0%) in T-ALL and CD33(85.7%), CDl3(74.3%) in AML, respectively. The incidence of acute mixed lineage leukemia(AMLL) was 26.7% and leukocytosis, anemia and thrombocytopenia were frequently seen in AMLL.
Conclusion: By the study of immunophenotyping we could more exactly diagnosed ALL and AML, as well as AMLL which was not exactly diagnosed by characteristics of morphology and cytochemistry only.
Therefore the best method for the diagnosis of acute leukemia will be achieved by using of immunophenotyping and FAB classification on the basis of morphology and
cytochemistry.
Keywords Acute leukemia; FAB; Immunophenotyping; Acute mixed-lineage leukemia;
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Korean J Hematol 1997; 32(1): 86-97
Published online March 31, 1997
Copyright © The Korean Society of Hematology.
급성 백혈병에서 면역학적 표지자의 표현양상
최현식, 김기연, 이중원, 서장수, 이원길, 김재식, 전동석
경북대학교 의과대학 임상병리학교실,
계명대학교 의과대학 임상병리학교실
Expression Patterns of Immunologic Surface Markers in Acute Leukemia
Huyn Sik Choi, Ki Youn Kim, Joong Won Lee, Jang Soo Suh, Won Kil Lee, Jay Sik Kim, Dong Seok Jean
Department of Clinical Pathology, College of Medicine, Kyungpook National University
Department of Clinical Pathology, Keimyung University, Taegu, Korea
Abstract
Background: Immunophenotyping is an important technique for the diagnosis and classification of acute leukemia, as well as French-American-British(FAB) classification
on the basis of morphologic characteristics and cytochemistry. We evaluated the expression patterns of immunologic surface markers in acute leukemia.
Methods: Peripheral or bone marrow leukemic cells from 75 leukemic patients(acute lymphoblastic leukemia, ALL 40 cases; children(26 cases), adults(14 cases) and acute
myeloid leukemia, AML 35 cases; children(9 cases), adults(26 cases)) were studied. Monoclonal antibodies which were designed for two color direct immunofluorescence(IF)
analysis with combination of fluoresceinisothiocynate(FITC) and phycoerythrin(PE) conjugated, CD10/CD19, CD20/CD5, CD3/CD22, CD7/CD33, HLA-DR/CD 13(Acute Leukemia Phenotyping Kit, Beckon Dickinson; BD, USA) were analyzed by flow
cytometry.
Results: Blasts from these patients could be classified as CALLA(+)B-ALL(26 cases, 65.0%), CALLA(-)B-ALL(6 cases, 15.0%), T-ALL(6 cases, 15.0%), biphenotypic ALL(2 cases, 5.0%). The positive expression rates were CDl9(100%), CD10(78.1%), CD22(75.0%) and CD20(50.0%) in B-ALL, CD7(100%), CD3(50.0%) and CD5(50.0%) in T-ALL and CD33(85.7%), CDl3(74.3%) in AML, respectively. The incidence of acute mixed lineage leukemia(AMLL) was 26.7% and leukocytosis, anemia and thrombocytopenia were frequently seen in AMLL.
Conclusion: By the study of immunophenotyping we could more exactly diagnosed ALL and AML, as well as AMLL which was not exactly diagnosed by characteristics of morphology and cytochemistry only.
Therefore the best method for the diagnosis of acute leukemia will be achieved by using of immunophenotyping and FAB classification on the basis of morphology and
cytochemistry.
Keywords: Acute leukemia, FAB, Immunophenotyping, Acute mixed-lineage leukemia,
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