Fig. 4. Cytotoxicity, cell cycling, and apoptosis in DLBCL. (A) The percentage of CD8⁺ T-cells lacking both granzyme B and perforin by PD-1 and TIM-3 expression (N=4). (B) The percentage of CD8⁺ T-cells that degranulate in response to PMA/ionomycin by PD-1, TIM-3, and CTLA-4 expression (N=4). (C) The percentage of CD8⁺ T-cells that are actively cycling by PD-1 and TIM-3 expression (N=4). (D) Percentage of CD8⁺ T-cells that are undergoing apoptosis by PD-1 and TIM-3 expression. Early apoptosis is defined as cleaved PARP⁺ viability dye-, and late apoptosis as cleaved PARP⁺ viability dye⁺ (N=4). P-values as indicated.

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