Table 1

Populations assessed using flow cytometry.

Cell type	Subpopulation	Immunophenotype
Myeloid	Monocyte	CD33⁺ CD14⁺ HLA-DR^hi
Myeloid	Monocytic myeloid derived suppressor cell	CD33⁺ CD14⁺ HLA-DRlo
B-cell CD19⁺ CD20⁺	Memory	CD24⁺ CD38^-
	Mature	CD24⁺ CD38⁺
	B10 regulatory	CD24^hi CD38^hi
Natural killer	NK	CD3^- CD56⁺
Natural killer	NK T-cell	CD3⁺ CD56⁺
CD4 T-cells CD3⁺ CD4⁺	Regulatory (Treg)	CD127^low CD25⁺ CXCR5^-
	PD-1^-	CXCR5^- PD-1^-
	PD-1^int	CXCR5^- PD-1^int
	Th1	CXCR5^- CCR6^- CXCR3⁺
	Th2	CXCR5^- CCR6^- CXCR3^-
	Th17	CXCR5^- CCR6⁺ CXCR3-
Follicular T-cells CD4⁺ CXCR5⁺ PD-1^hi	Follicular helper (Tfh), CD10⁺	CD10⁺
	Follicular helper, CD10^-	CD10^-
	Follicular regulatory (Tfr)	CD127^low CD25⁺
	Tfh-Th1	CCR6^- CXCR3⁺
	Tfh-Th2	CCR6^- CXCR3^-
	Tfh-Th17	CCR6⁺ CXCR3^-
CD8 T-cells CD3⁺ CD8⁺	Regulatory (Treg)	CD127^low CD25⁺ CXCR5^-
	PD-1^-	CXCR5^- PD-1^-
	PD-1⁺	CXCR5^- PD-1⁺
	CXCR5⁺	CXCR5⁺
Dendritic cell	Myeloid dendritic cell	CD14^- CD33⁺ HLA-DR⁺
Dendritic cell	Pre-dendritic cells	CD14⁺ CD33- HLA-DR⁺

Shown are the cell populations analyzed, their subpopulations, and their immunophenotypes.