Primers and probe sequences for the CTA duplex qRT-PCR assays.
MAGEA3/ABL duplex (NM_5362.3)a) | |
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CTA primers | TCTTGAGCAGAGGAGTCAGCAC (F); GATCTGGTGACTCGGGAGCA (R) |
CTA probe | 56-FAM/CTCCCCCAG/ZEN/GGTGACTTCAACTA/3IABkFQ |
ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
GATGTAGTTGCTTGGGACCCA (R) | |
ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
Amplicon | 176 bp (MAGEA3)+124 bp (ABL) |
PRAME/ABL duplex (NM_6115.4) | |
CTA primers | CTGTGCTTGATGGACTTGATGTG (F) |
GCTGCTCTGCCTCTGTGCTC (R) | |
CTA probe | 56-FAM/ACCATCTAC/ZEN/TTTTCGCTTCTTTGTCATGGG/3IABkFQ |
ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
GATGTAGTTGCTTGGGACCCA (R) | |
ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
Amplicon | 216 bp (PRAME)+124 bp (ABL) |
BAGE2/ABL duplex (NM_1839676.1) | |
CTA primers | CGGCCAGAGCGGTTTTT (F) |
CTCCTCCTATTGCTCCTGTTG (R) | |
CTA probe | 56-FAM/CGTCTCCAT/ZEN/CACCGTGGCTGCCACAA/IABkFG |
ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
GATGTAGTTGCTTGGGACCCA (R) | |
ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
Amplicon | 150 bp (BAGE2)+124 bp (ABL) |
a)GenBank ref sequence ID for CTA gene.