Fig. 1. Flow cytometric platelet aggregation of a healthy control. Dot plots showing platelets in unstimulated (t=0 min) and ADP-stimulated state (t=10 min) in a healthy control (left and right, respectively). The platelets are gated on a log forward (FSC) and side scatter (SSC) (upper panel) and examined on CD31-FITC and CD31-PE combination (lower panel). The double-colored events (Q2) represent the platelet aggregates, Q1 represents the CD31-PE-labeled platelets, Q4 represents the CD31-FITC-labeled platelets, and Q3 represents the unstained events (plasma, debris, etc.). The % double-colored events in this control in unstimulated t=0 min and ADP-stimulated platelet mix at t=10 min are 1.8% and 41.4% respectively.

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