Table 5

Molecular diagnostic methods for thalassemia.

Mutation type	Method
Deletion	Gap PCR
Deletion	MLPA^a,b)
Non-deletion	Allele-specific PCR
	Reverse dot blotting
	Denaturing gradient gel electrophoresis
	ARMS
	Sanger sequencing^a)
	Next-generation sequencing^a)

^a)Methods currently applied in clinical laboratories in Korea. ^b)Commercial kits available [SALSA MLPA Probemix P140 and P102 (MRC-Holland, Amsterdam, The Netherlands)].

Abbreviations: ARMS, amplification refractory mutation system; MLPA, multiplex ligation-dependent probe amplification; PCR, polymerase chain reaction.

Blood Res 2021;56:S39~S43 https://doi.org/10.5045/br.2021.2020332