Fig. 2. A representative gel shows genotyping results of the CROM*01.12 and CROM*01.-12 alleles using the PCR-SSP technique. The 434 bp amplification product of the HGH control primers was present in all lanes, showing that amplification had optimally occurred. The genotype was deduced from the presence or absence of 235 bp amplification product specific for CROM*01.12 and CROM*01.-12 alleles [from left to right, lanes 1A-1B=CROM*01.12/CROM*01.12; 2A-2B=CROM*01.12/CROM*01.-12 and NC=negative control, respectively; M, 100 bp ladder marker (Fermentas, Carlsbad, CA, USA)].

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