Fig. 2. Preparations of “diluted” or “mixed” serum and paired comparisons among them. Recipient serum was mixed with an equal volume of donor serum (mixed serum) and incubated at 25°C for 30 min. For comparison with mixed serum, the recipient serum was diluted with an equal volume of diluent, either RPMI or third-party serum (diluted serum). The green symbols resembling an uppercase letter Y represent antibodies, and the small round or triangular symbols represent sHLA. In the mixed serum, the sHLA (green round dots) from a donor (previous sensitizer) can neutralize donor-specific antibodies (green “Y”) in recipient serum.

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