Fig. 1. Bone marrow biopsy showing (A) hypercellular marrow with increased megakaryocytes and atypia (H&E stain, ×400) and (B) myelofibrosis grade MF-2 demonstrated by reticulin staining (reticulin stain, ×200). Reverse transcriptase (RT)-PCR analysis of BCR-ABL1 translocation using a HemaVision kit showing a single 397 bp band in the M6B split out PCR, indicating a fusion transcript with BCR-ABL1(C). Sequencing analysis of the RT-PCR product showing breakpoint located at exon 13 in the BCR gene and exon 2 in the ABL gene (D). Sequencing analysis of JAK2V617F showing a heterozygous JAK2V617F mutation (E).