PCR products were subjected to electrophoresis. IKZF1-associated bands are visible at the 200 bp marker. (A) MSP results with IKZF1 unmethylated primers for the control group. As can be seen, in most of the control samples, the unmethylated pattern was observed. (B) MSP results with the methylated primers of the IKZF1 gene for the control group. As shown in this figure, the majority of these samples exhibited a relative methylation state. (C) MSP results with IKZF1-specific methylated primer for the patient group. As can be seen, samples from patients didn't have any band with methylated primer. (D) MSP results with IKZF1 unmethylated primers for the patient group. All samples of the patients group had recognizable bands with the unmethylated primers of IKZF1 promoter.