Reconstitution of human cells in newborn NSG mice after intrahepatic transplantation of CD34⁺ cells. Mononuclear cells derived from bone marrow, spleen, peripheral blood and liver tissues of humanized NSG mice were isolated in the 12th week of transplantation and were stained with anti-hCD45, anti-hCD3, and anti-hCD19 antibodies. Data are means±SEM and representative of four mice per group, excluding lowest values (N=5).

Abbreviations: BM, bone marrow; LV, liver; ND, not detected; PB, peripheral blood; SPL, spleen.