(A–C) Relative expression levels of Oct4, Nanog, and Brachyury, respectively on days 8 and 11. (D, E) Flow cytometric analysis of KDR+ cells after 4 days of differentiation (D) Histogram images of the KDR flow cytometry data. (E) The percentage of KDR+ cells in the total cell population. (F) Relative expression of Hoxb4 toward that in undifferentiated ESCs. a)P <0.05, b)P <0.01, c)P <0.001.