BLOOD RESEARCH

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Fig. 1.

Principles of MEMO-PCR for MYD88 L265P detection. Blocking primers, complementary to the normal sequence, anneal to normal DNA, hampering PCR amplification. In the presence of a missense mutation, the binding affinity of the blocking primers to the mutant DNA is decreased due to the mismatch, and therefore, amplification of the mutant DNA is markedly enhanced.

Blood Res 2016;51:181~186 https://doi.org/10.5045/br.2016.51.3.181
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