Blood Res 2018; 53(4): 320-324  https://doi.org/10.5045/br.2018.53.4.320
Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings
Abdolrahim Ahmadi1, Saeid Kaviani1#, Marjan Yaghmaie2,3,4#, Hossein Pashaiefar2,3,4, Mohammad Ahmadvand2,3,4, Mahdi Jalili2,3,4, Kamran Alimoghaddam2,3,4, Mohammad Eslamijouybari5, Ardeshir Ghavamzadeh2,3,4
1Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, 2Hematology–Oncology and Stem Cell Transplantation Research Center, 3Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, 4Hematologic Malignancies Research Center, Tehran University of Medical Sciences, Tehran, 5Comprehensive Cancer Research Center, Mazandaran University of Medical Science, Sari, Iran
Correspondence to: Saeid Kaviani, M.D., Marjan Yaghmaie, M.D.
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, P.O.Box: 14115-331, (S.K.), Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran, P.O.Box: 1411713135 (M.Y.)
E-mail: S.K., kavianis@modares.a.ir, M.Y., m-yaghmaie@sina.tums.ac.ir
Received: June 7, 2018; Revised: August 27, 2018; Accepted: September 5, 2018; Published online: December 31, 2018.
© The Korean Journal of Hematology. All rights reserved.

cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0)which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background
Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.
Methods
MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH).
Results
MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients.
Conclusion
Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.
Keywords: MALAT1, Chronic lymphocytic leukemia , qRT-PCR, FISH


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