Blood Res 2018; 53(1): 61-70  https://doi.org/10.5045/br.2018.53.1.61
Expression of adhesion molecules on CD34+ cells from steady-state bone marrow before and after mobilization and their association with the yield of CD34+ cells
Karin Zattar Cecyn, Eliza Y.S. Kimura, Dulce Marta S.M. Lima, Miyoko Yamamoto, José Orlando Bordin, José Salvador R. de Oliveira
Oncologia Clínica e Experimental, Universidade Federal de São Paulo – UNIFESP, Sao Paulo, Brazil
Correspondence to: Karin Zattar Cecyn, Ph.D. Oncologia Clínica e Experimental, Universidade Federal de São Paulo – UNIFESP, Rua Diogo de Faria-824, Vila Clementino, Sao Paulo, Brazil E-mail: kcecyn@gmail.com
Received: May 24, 2017; Accepted: December 14, 2017; Published online: March 31, 2018.
© The Korean Journal of Hematology. All rights reserved.

Abstract
Background Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors. Methods Three-color cytofluorometric analysis was used to compare the expressions of CAMs and chemokines in the bone marrow before and after mobilization. Results For all studied groups, CAM expression among those with good and poor yields of CD34+ cells was significantly correlated with VCAM-1 (P=0.007), CD44 (P=0.027), and VLA-4 (P=0.014) expressions. VCAM-1 (P=0.001), FLT-3 (P=0.001), CD44 (P=0.011), VLA-4 (P=0.001), and LFA-1 (P=0,001) expressions were higher before HPC mobilization than after HPC mobilization. By contrast, the expression of CXCR4 significantly varied before and after mobilization only among those with successful PBSC mobilization (P=0.002). Conclusion We attempted to identify particular aspects of CAMs involved in CD34+ cell mobilization, which is a highly complex mechanism that involves adhesion molecules and matrix metalloproteases. The mechanism by which CD34+ cell mobilization is activated through proteolytic enzymes is not fully understood. We believe that CXCR4, VLA-4, CD44, and VCAM-1 are the most important molecules implicated in HPC mobilization, particularly because they show a correlation with the yield of CD34+ cells collected via large volume leukapheresis.
Keywords:
Adhesion molecules, Hematopoietic progenitor cells, Mobilization, Stem cell donor, Multiple myeloma, Non-Hodgkin lymphoma


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