Blood Res 2015; 50(1):
Published online March 31, 2015
https://doi.org/10.5045/br.2015.50.1.26
© The Korean Society of Hematology
1Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Medical University of Warsaw, Warsaw, Poland.
2Department of Pediatric Haematology & Oncology, Medical University of Warsaw, Warsaw, Poland.
Correspondence to : Correspondence to Ewa Musialik, M.S. Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, W.K. Roentgena 5, 02-781 Warsaw, Poland. Tel: +48-22-546-28-98, Fax: +48-22-546-31-91, musialik.ewa@gmail.com
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Precursor B-cell acute lymphoblastic leukemia (B-cell ALL) is the most common neoplasm in children and is characterized by genetic and epigenetic aberrations in hematopoietic transcription factor (TF) genes. This study evaluated promoter DNA methylation and aberrant expression levels of early- and late-acting hematopoietic TF genes homeobox A4 and A5 (
Blood samples of 38 ALL patients and 20 controls were obtained. DNA was treated with sodium bisulfite and DNA methylation level of
Aberrant methylation of
Aberrant methylation and expression of the selected hematopoietic genes were correlated with demographic/clinical prognostic factors of pediatric ALL, such as age, WBC count, and NCI risk classification.
Keywords DNA methylation, Acute lymphoblastic leukemia, Gene expression, Transcription factors
Blood Res 2015; 50(1): 26-32
Published online March 31, 2015 https://doi.org/10.5045/br.2015.50.1.26
Copyright © The Korean Society of Hematology.
Ewa Musialik1,#*, Mateusz Bujko1,#, Paulina Kober1, Agnieszka Wypych2, Karolina Gawle-Krawczyk2, Michal Matysiak2, and Janusz Aleksander Siedlecki1
1Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Medical University of Warsaw, Warsaw, Poland.
2Department of Pediatric Haematology & Oncology, Medical University of Warsaw, Warsaw, Poland.
Correspondence to: Correspondence to Ewa Musialik, M.S. Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, W.K. Roentgena 5, 02-781 Warsaw, Poland. Tel: +48-22-546-28-98, Fax: +48-22-546-31-91, musialik.ewa@gmail.com
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Precursor B-cell acute lymphoblastic leukemia (B-cell ALL) is the most common neoplasm in children and is characterized by genetic and epigenetic aberrations in hematopoietic transcription factor (TF) genes. This study evaluated promoter DNA methylation and aberrant expression levels of early- and late-acting hematopoietic TF genes homeobox A4 and A5 (
Blood samples of 38 ALL patients and 20 controls were obtained. DNA was treated with sodium bisulfite and DNA methylation level of
Aberrant methylation of
Aberrant methylation and expression of the selected hematopoietic genes were correlated with demographic/clinical prognostic factors of pediatric ALL, such as age, WBC count, and NCI risk classification.
Keywords: DNA methylation, Acute lymphoblastic leukemia, Gene expression, Transcription factors
Analysis of promoter methylation and expression of genes encoding hematopoietic TFs in pediatric patients with B-cell ALL. (
Table 1 . Characteristics of patients with acute lymphoblastic leukemia..
Abbreviations: ALL, acute lymphoblastic leukemia; BM, bone marrow; MLL, mixed-lineage leukemia; PB, peripheral blood..
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