Quality of cord blood cryopreserved for up to 5 years

Hye Ryun Lee; Eun Young Song; Sue Shin; Eun Youn Roh; Jong Hyun Yoon; Byoung Jae Kim

doi:10.5045/br.2014.49.1.54

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Original Article

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Blood Res 2014; 49(1):

Published online March 31, 2014

https://doi.org/10.5045/br.2014.49.1.54

Quality of cord blood cryopreserved for up to 5 years

Hye Ryun Lee¹, Eun Young Song², Sue Shin^2,3,5*, Eun Youn Roh^2,3,5, Jong Hyun Yoon^2,3,5, and Byoung Jae Kim⁴

Author Info. +

¹Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.

²Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

³Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.

⁴Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea.

⁵Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.

Correspondence to : Correspondence to Sue Shin, M.D. Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, Seoul 156-707, Korea. Tel: +82-2-870-2602, Fax: +82-2-870-2620, Jeannie@snu.ac.kr

Received: September 9, 2013; Revised: December 11, 2013; Accepted: February 25, 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Abstract

Background

Although cord blood (CB) is a well-known source of hematopoietic stem cells, uncertainties exist regarding the quality of cryopreserved CB. We investigated the changes in quality of CB units according to the duration of cryopreservation.

Methods

We analyzed CB units that were rejected from the Seoul Metropolitan Government Public Cord Blood Bank inventory after conventional processing, because of unsuitability for allogeneic transplantation. Two hundred CB units that were cryopreserved from 1 year to 5 years were selected. After thawing the cryopreserved CB units, the total nucleated cell (TNC) count, CD34+ cell count, number of colony-forming units (CFU), aldehyde dehydrogenase (ALDH) level, cell viability, and apoptosis were analyzed. We conducted a comparative analysis to identify the presence of statistically significant differences in the recovery rates of the TNC and CD34+ cell counts and to compare the results of ALDH level, the cell viability test, the apoptosis test, and CFU analysis among groups according to the duration of cryopreservation.

Results

The recovery rates of the TNC count, the CD34+ cell count, and cell viability did not differ significantly according to the duration of cryopreservation. ALDH analysis, the cell viability test, and the apoptosis test did not reveal any increasing or decreasing trend according to the duration of cryopreservation. Further, the numbers of CFU-granulocyte/macrophage and CFU-granulocyte/erythrocyte/macrophage/megakaryocyte did not differ significantly according to the duration of cryopreservation.

Conclusion

These results suggest that the quality of CB is not affected by cryopreservation for up to a period of 5 years.

Keywords Cord blood, Cryopreservation, Quality

Article

Original Article

Blood Res 2014; 49(1): 54-60

Published online March 31, 2014 https://doi.org/10.5045/br.2014.49.1.54

Quality of cord blood cryopreserved for up to 5 years

Hye Ryun Lee¹, Eun Young Song², Sue Shin^2,3,5*, Eun Youn Roh^2,3,5, Jong Hyun Yoon^2,3,5, and Byoung Jae Kim⁴

¹Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.

²Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

³Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.

⁴Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea.

⁵Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.

Correspondence to: Correspondence to Sue Shin, M.D. Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, Seoul 156-707, Korea. Tel: +82-2-870-2602, Fax: +82-2-870-2620, Jeannie@snu.ac.kr

Received: September 9, 2013; Revised: December 11, 2013; Accepted: February 25, 2014

Abstract

Background

Methods

Results

Conclusion

These results suggest that the quality of CB is not affected by cryopreservation for up to a period of 5 years.

Keywords: Cord blood, Cryopreservation, Quality

Abstract

Table 1 . The number of total nucleated cells (TNC) before and after cryopreservation and TNC recovery according to the duration of cryopreservation..

Plus-minus values (±) are standard deviation of the mean..

^a)While recovery after thawing was sometimes greater than 100%, we chose to use an upper limit of recovery of 100%..

Table 2 . The number of CD34+ cells before and after cryopreservation and CD34+ cell recovery according to the duration of cryopreservation..

Plus-minus values (±) are standard deviation of the mean..

Table 3 . Cell viability before and after cryopreservation according to the duration of cryopreservation..

Plus-minus values (±) are standard deviation of the mean..

^a)The change between the viability before freezing and the viability after thawing..

Table 4 . Comparative analysis of the results according to the duration of cryopreservation..

Plus-minus values (±) are standard deviation of the mean..

^a)Cells with high ALDH activity among CD34+ cells. ^b)Cells with high ALDH activity. ^c)CD34+ cells among cells with high ALDH activity..

Abbreviations: ALDH, aldehyde dehydrogenase; TNC, total nucleated cells; 7-AAD, 7-aminoactinomycin D; CFU-GEMM, colony-forming unit-granulocyte/erythrocyte/macrophage/megakaryocyte; CFU-GM, colony-forming unit-granulocyte/macrophage..