Korean J Hematol 2007; 42(4):
Published online December 30, 2007
https://doi.org/10.5045/kjh.2007.42.4.392
© The Korean Society of Hematology
서인범, 유숙원, 이용구, 허대성, 정찬일, 장준근, 임채승
강원대학교 의과대학 진단검사의학교실,
주, 디지털바이오테크놀러지,
서울대학교 공과대학 기계항공공학부, 전기컴퓨터공학부,
고려대학교 의과대학 진단검사의학교실
Background:
Blood typing is an essential test for transfusion. Generally, blood typing is performed using a slide test, tube test or microcolumn agglutination test. The aims of this study were to develop a new blood typing kit using micromachining, microfluidics and microseparation methods, and to evaluate the clinical usefulness of the new blood typing kit.
Methods:
We designed and manufactured a blood typing microchip using polydimethylsiloxane (PDMS), which contained a microchannel (25∼200Ռm). The blood sample and antisera to be tested were dropped on the microwell for movement and mixing by capillary action. Once agglutination occurred, the microchannel acts as a filter and the blood type was determined by observation by the naked eye. To evaluate the newtyping kit, we tested sensitivity using artificially diluted blood and compared the results of the new typing method with the slide and tube methods using 70 samples.
Results:
The new blood typing kit could differentiate a +4∼+2 agglutination reaction, but could not detect a +1 agglutination reaction as observed by the naked eye. Among 70 samples, the results of ABO and Rh typing by the new typing method (n=66, ≥+2 agglutination reaction by the column agglutination method) were in accord with the results of the tube and slide methods, but couldnot detect agglutination in all 4 clinical samples, below a +1 agglutination reaction.
Conclusion:
he new blood typing kit is inadequate for routine use in the clinical laboratory due to low sensitivity, but with further improvement, it can be used economically, conveniently and objectively for blood typing without any special equipment. Moreover, the microfludics and separation method may be broadly applicable in other tests using the hemagglutination method.
Keywords ABO, Rh, Blood typing, Microfluidics, Microseparation
Korean J Hematol 2007; 42(4): 392-396
Published online December 30, 2007 https://doi.org/10.5045/kjh.2007.42.4.392
Copyright © The Korean Society of Hematology.
서인범, 유숙원, 이용구, 허대성, 정찬일, 장준근, 임채승
강원대학교 의과대학 진단검사의학교실,
주, 디지털바이오테크놀러지,
서울대학교 공과대학 기계항공공학부, 전기컴퓨터공학부,
고려대학교 의과대학 진단검사의학교실
In Bum Suh, Sook Won Ryu, Yong ku Lee, Dae Sung Hur, Chanil Chung, Jun Keun Chang, Chae Seung Lim
Department of Laboratory Medicine, Kangwon National University College of Medicine, Chuncheon
Digital Bio Technology Co
Seoul National University School of Mechanical and Aerospace Engineering
Seoul National University School of Electrical Engineering and Computer Science
Department of Laboratory Medicince, Korea University College of Medicine, Seoul, Korea
Background:
Blood typing is an essential test for transfusion. Generally, blood typing is performed using a slide test, tube test or microcolumn agglutination test. The aims of this study were to develop a new blood typing kit using micromachining, microfluidics and microseparation methods, and to evaluate the clinical usefulness of the new blood typing kit.
Methods:
We designed and manufactured a blood typing microchip using polydimethylsiloxane (PDMS), which contained a microchannel (25∼200Ռm). The blood sample and antisera to be tested were dropped on the microwell for movement and mixing by capillary action. Once agglutination occurred, the microchannel acts as a filter and the blood type was determined by observation by the naked eye. To evaluate the newtyping kit, we tested sensitivity using artificially diluted blood and compared the results of the new typing method with the slide and tube methods using 70 samples.
Results:
The new blood typing kit could differentiate a +4∼+2 agglutination reaction, but could not detect a +1 agglutination reaction as observed by the naked eye. Among 70 samples, the results of ABO and Rh typing by the new typing method (n=66, ≥+2 agglutination reaction by the column agglutination method) were in accord with the results of the tube and slide methods, but couldnot detect agglutination in all 4 clinical samples, below a +1 agglutination reaction.
Conclusion:
he new blood typing kit is inadequate for routine use in the clinical laboratory due to low sensitivity, but with further improvement, it can be used economically, conveniently and objectively for blood typing without any special equipment. Moreover, the microfludics and separation method may be broadly applicable in other tests using the hemagglutination method.
Keywords: ABO, Rh, Blood typing, Microfluidics, Microseparation
Nitin Agarwal, Rakesh Mohan Thapliyal, and Kabita Chatterjee
Blood Res 2013; 48(1): 51-54